Superose 6 pc 3.2 300 column

Manufactured by GE Healthcare

Sourced in Sweden

The Superose 6 PC 3.2/300 column is a size exclusion chromatography column designed for the separation and purification of proteins, peptides, and other biomolecules. The column has a bed volume of 2.4 mL and a column dimension of 3.2 mm x 300 mm. It utilizes a cross-linked agarose-based packing material to achieve effective separation based on molecular size.

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Lab products found in correlation

Cholesterol chod pap, by Roche (1 mentions) Tg gpo pap, by Roche (1 mentions) Superose 6 10 300 gl column, by GE Healthcare (1 mentions)

Spelling variants of this product

Superose 6 column (200 citations) Superose 6 (82 citations) Superose 6 3.2/300 column (16 citations) Superose 6 3.2/300 (5 citations) Superose 3.2/300 column (2 citations)

4 protocols using superose 6 pc 3.2 300 column

Comprehensive Lipid and Carnitine Profiling

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Lipids from plasma were measured enzymatically on a Hitachi 917 system (Roche Diagnostics GmbH, Mannheim, Germany) using the cholesterol (CHOD-PAP) and TAG (GPO-PAP) kit from Roche Diagnostics, and the free cholesterol (Free Cholesterol FS), non-esterified fatty acid (NEFA FS,) and phospholipid kit (Phospholipids FS) from DiaSys (Diagnostic Systems GmbH, Holzheim, Germany). Lipoproteins were also separated from 2.5 μL of individual plasma samples by size exclusion chromatography (SEC), using a Superose 6 PC 3.2/300 column (GE Healthcare Bio-Sciences AB, Uppsala, Sweden). Lipoproteins were eluted as a fraction appearing in the exclusion volume of the sepharose column that contained chylomicrons (if present) together with VLDL, then LDL and last HDL. Total cholesterol was calculated after integration of the AUC in the individual chromatograms [52 (link),53 (link),54 (link)], generated by the enzymatic-colorimetric reaction Cholesterol CHOD-PAP (Roche Diagnostics).
L-carnitine, trimethyllysine, γ-butyrobetaine, acetylcarnitine, propionylcarnitine, valerylcarnitine, octanoylcarnitine, lauroylcarniitne, myristoylcarnitine, palmitoylcarnitine, betaine, choline and TMAO were analyzed in plasma by HPLC-MS/MS as described by Vernez et al. [55 (link)] with some modifications [56 (link)].

Aloysius T.A., Tillander V., Pedrelli M., Dankel S.N., Berge R.K, & Bjørndal B. (2022). Plasma Cholesterol- and Body Fat-Lowering Effects of Chicken Protein Hydrolysate and Oil in High-Fat Fed Male Wistar Rats. Nutrients, 14(24), 5364.

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Lipoprotein Separation and Triglyceride Quantification

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Lipoproteins were separated from 2.5 μl of individual plasma samples by size exclusion chromatography using a Superose 6 PC 3.2/300 column (GE Healthcare Bio-Sciences AB, Uppsala, Sweden). Lipoproteins were eluted as a fraction appearing in the exclusion volume of the sepharose column that contained VLDL, then LDL, and lastly HDL. TG concentrations were calculated after integration of the individual chromatograms (50 (link), 51 (link)), generated by the enzymatic-colorimetric reaction with the respective following kits, TG GPO-PAP (Roche Diagnostics, Mannheim, Germany).

Delbès A.S., Quiñones M., Gobet C., Castel J., Denis R.G., Berthelet J., Weger B.D., Challet E., Charpagne A., Metairon S., Piccand J., Kraus M., Rohde B.H., Bial J., Wilson E.M., Vedin L.L., Minniti M.E., Pedrelli M., Parini P., Gachon F, & Luquet S. (2023). Mice with humanized livers reveal the role of hepatocyte clocks in rhythmic behavior. Science Advances, 9(20), eadf2982.

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Analytical SEC of Protein Complexes

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Analytical SEC experiments were performed as previously described (9 (link), 32 (link)). Briefly, either a Superose 6 PC 3.2/300 column (GE Healthcare) for in vitro translated p63 or a Superose 6 GL 10/300 column (GE Healthcare) for cell lysates of transiently transfected H1299 cells were used. H1299 cell lysate was prepared by three cycles of freezing and thawing in SEC running buffer (50 mM Tris pH 7.5; 150 mM NaCl; 1 mM DTT) with protease inhibitors and Benzonase followed by removal of cell debris by centrifugation. Before injection, cell lysates were incubated at 37 °C for 15 min. The Superose 6 GL 10/300 column was calibrated using the Gel Filtration HMW and LMW Calibration Kits.

Russo C., Osterburg C., Sirico A., Antonini D., Ambrosio R., Würz J.M., Rinnenthal J., Ferniani M., Kehrloesser S., Schäfer B., Güntert P., Sinha S., Dötsch V, & Missero C. (2018). Protein aggregation of the p63 transcription factor underlies severe skin fragility in AEC syndrome. Proceedings of the National Academy of Sciences of the United States of America, 115(5), E906-E915.

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Size Exclusion Chromatography of Rabbit Reticulocyte Lysate Proteins

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Size exclusion chromatography experiments with proteins expressed in rabbit reticulocyte lysate transcription/ translation system (Promega) were performed as previously described (Deutsch et al., 2011) at 4 C using a Superose 6 PC 3.2/300 column (GE Healthcare) with an injection volume of 50mL and a flow rate of 50mL/ min. Prior to injection, samples were spun down at 13.2 krpm for 15 min and 4 C. 45mL of RRL lysate was mixed with 35mL of Phosphate running buffer (9.08 mM Na2HPO4, 0.92 mM NaH2PO4, 200 mM NaCl). The sample was separated with a flow rate of 0.05 mL/min and eluates were collected between 1.0 mL and 1.7 mL elution volume in fractions of 50 mL. Samples for Western Blot analysis were prepared in a 96-microwell-PCR plate. 10 mL of SDS sample buffer were provided in each well and 30 mL of the elution fraction were added. 10mL of sample were used for Western Blot analysis.

Krauskopf K., Gebel J., Kazemi S., Tuppi M., Löhr F., Schäfer B., Koch J., Güntert P., Dötsch V, & Kehrloesser S. (2018). Regulation of the Activity in the p53 Family Depends on the Organization of the Transactivation Domain. Structure (London, England : 1993), 26(8).

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