Mouse anti rsv n

For immunostaining, primary antibodies used were mouse anti-RSV N (Abcam, Cat. No. ab22501), mouse anti-RSV G (Abcam, Cat. No. ab94966), rabbit anti-camelid VHH (GenScript, Cat. No. A01860-200), goat anti-panRSV polyclonal (Millipore, Cat. No. AB1128), rat anti-CD63 (BD, Cat. No. 564221), rabbit anti-Rab5 (Affinity BioReagents, Cat. No. PA3-915), and rabbit anti-Rab7 (Abcam, Cat. No. 137029). All primary antibodies for immunostaining experiments were used at 1 µg/mL. Secondary antibodies used were donkey anti-human Alexa Fluor 647 (Jackson ImmunoResearch), donkey anti-goat Alexa Fluor 546, donkey anti-rabbit Alexa Fluor 488, and donkey anti-mouse Alexa Fluor 488 (all from Life Technologies). All secondary antibodies for immunostaining experiments were used at 4 µg/mL. For dSTORM experiments, secondary antibodies used were donkey anti-mouse Alexa Fluor 647 (Life Technologies) and goat anti-human CF568 (Biotium), and both were used at 1 µg/mL.
For western blot, the primary antibodies used were a rabbit monoclonal anti phosphorylated eIF2α (Cell Signaling Technologies, Cat. No. 9721) and goat anti-pan-eIF2α (R&D Systems, Cat. No. AF3997) diluted 1:1000 in 5% BSA in TBS with 0.1% Tween-20. The secondary antibodies were a donkey anti-mouse IRDye 680RD (LI-COR) and a donkey anti-rabbit IRDye 800 (LI-COR) and were diluted 1:5000 in 5% BSA in TBS with 0.1% Tween-20.

For western blot, the primary antibody used was a mouse monoclonal anti-RSV G (Abcam, Cat. No. ab94966) diluted to 2 µg/mL in Odyssey blocking buffer (LI-COR) with 0.1% Tween-20. The secondary antibodies were a donkey anti-mouse IRDye 680RD (LI-COR) and a donkey anti-rabbit IRDye 800 (LI-COR) and were diluted 1:3000 in Odyssey blocking buffer with 0.1% Tween-20.
For immunostaining, primary antibodies used were mouse anti-RSV N (Abcam, Cat. No. ab22501), human anti-RSV F (MedImmune, palivizumab), mouse anti-RSV F (Abcam, Cat. No. ab94968), mouse anti-RSV G (a gift from Ralph Tripp, clone 130-6D), rabbit anti-caveolin-1 (Santa Cruz, Cat. No. sc-894), and mouse anti-clathrin light chain (Biolegend, Cat. No. MMS-423P). The mouse anti-RSV F antibody was used only for the mRNA expression experiments in Fig. 1. All primary antibodies for immunostaining experiments were used at 1 µg/mL. Secondary antibodies used were donkey anti-rabbit Cy3 (Jackson ImmunoResearch), donkey anti-human Alexa Fluor 647, and donkey anti-mouse Alexa Fluor 488 (Life Technologies). All secondary antibodies for immunostaining experiments were used at 4 µg/mL.