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Plvx shrna lentiviral vectors

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PLVX-shRNA lentiviral vectors are a type of lab equipment designed for the expression of short hairpin RNA (shRNA) sequences in target cells. These vectors utilize the lentiviral delivery system to efficiently introduce shRNA constructs into a variety of cell types, including both dividing and non-dividing cells. The core function of these vectors is to facilitate the silencing or knockdown of specific gene expression in the transduced cells.

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PLVX vector (22 citations) PLVX lentiviral vector (9 citations) PLVX-shRNA (7 citations) PLVX-shRNA vector (2 citations)

2 protocols using plvx shrna lentiviral vectors

1

Lentiviral Vector Production for shRNA

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The shRNAs encoding siN159D-6 and scrambled siRNA (siN159D-6S) were cloned into pLVX-shRNA lentiviral vectors (Clontech, Mountain View, CA) between Pst I and BamH I sites (Supplemental Figure S2). The lentiviral vectors were produced in 293T cells as described elsewhere (Yasuda et al., 2013 (link)). Viral vectors were dissolved in serum-containing medium, aliquoted in single-use vials, and stored at −80°C.
Liu Y., Snedecor E.R., Zhang X., Xu Y.F., Huang L., Jones E., Zhang L., Clark R.A., Roop D.R., Qin C, & Chen J. (2016). Correction of hair shaft defects through allele-specific silencing of mutant Krt75. The Journal of investigative dermatology, 136(1), 45-51.
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2

Lentiviral Vector Production for shRNA

Check if the same lab product or an alternative is used in the 5 most similar protocols
The shRNAs encoding siN159D-6 and scrambled siRNA (siN159D-6S) were cloned into pLVX-shRNA lentiviral vectors (Clontech, Mountain View, CA) between Pst I and BamH I sites (Supplemental Figure S2). The lentiviral vectors were produced in 293T cells as described elsewhere (Yasuda et al., 2013 (link)). Viral vectors were dissolved in serum-containing medium, aliquoted in single-use vials, and stored at −80°C.
Liu Y., Snedecor E.R., Zhang X., Xu Y.F., Huang L., Jones E., Zhang L., Clark R.A., Roop D.R., Qin C, & Chen J. (2016). Correction of hair shaft defects through allele-specific silencing of mutant Krt75. The Journal of investigative dermatology, 136(1), 45-51.
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