Human hnp 1 3 enzyme linked immunosorbent assay elisa reagents

Dulbecco’s phosphate-buffered saline (D-PBS) and TRIzol^® reagent were purchased from Invitrogen (Carlsbad, CA, USA), the IsoPrep RNeasy total RNA kit was from Qiagen (Hilden, Germany), and the Affymetrix GeneChip^® Human Gene 1.0 ST Array was from Affymetrix (Santa Clara, CA, USA). Quantitative reverse transcription (qRT)-PCR primers were designed using Primer3 (v.0.4.0) software, GenBank sequences and based on previous studies, and were synthesized by Pacific Science Co., Ltd. (Bangkok, Thailand). The specificity of the primers for the target genes was also determined using the BLAST program [16 (link)]. Human HNP 1–3 enzyme-linked immunosorbent assay (ELISA) reagents were purchased from Hycult^® Biotech (Uden, the Netherlands). All other reagents were from Sigma-Aldrich (St. Louis, MO, USA).

In this study we used D-PBS (Wisent Inc., Quebec, Canada), TRIzol^® Reagent (Invitrogen™, Carlsbad, CA, USA), IsoPrep (Robbins Scientific Corporation, Sunnyvale, CA, USA), RNeasy total RNA kit (Qiagen, Hilden, Germany), and the Affymetrix GeneChip® Human Gene 1.0 ST Arrays (Affymetrix, Santa Clara, CA, USA). For qRT-PCR, we designed primers based on previous studies and Genbank, and had primers synthesized by Pacific Science Co., Ltd. (Bangkok, Thailand). Human HNP 1–3 enzyme-linked immunosorbent assay (ELISA) reagents were purchased from Hycult^® Biotech (Uden, The Netherlands). All other reagents were from Sigma-Aldrich (St. Louis, MO, USA).