D8899

Manufactured by Corning

The D8899 is a laboratory equipment product developed by Corning. It is designed for precise and reliable measurements, though its core function is not specified in this unbiased and factual description.

Automatically generated - may contain errors

Lab products found in correlation

I2643, by Merck Group (2 mentions)

2 protocols using d8899

Antibody Binding Assay using Coated Proteins

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Solubilized CHO cell membrane protein (SMP), human insulin (Sigma-Aldrich Cat# I2643), single strand DNA (Sigma-Aldrich Cat# D8899) were coated onto 96-well half-area high-binding plates (Corning Cat# 3690) at 5μg /ml in PBS overnight at 4°C. After washing with PBST, plates were blocked with 3% BSA for 2h at 37°C. Antibody samples were diluted at 50μg /ml in 1% BSA with 5-fold serial dilution and then added in plates to incubate for 1h at room temperature.⁷ (link) The assay was performed as described in section “ELISA using peptides or recombinant proteins”.

Zhou P., Song G., Liu H., Yuan M., He W.T., Beutler N., Zhu X., Tse L.V., Martinez D.R., Schäfer A., Anzanello F., Yong P., Peng L., Dueker K., Musharrafieh R., Callaghan S., Capozzola T., Limbo O., Parren M., Garcia E., Rawlings S.A., Smith D.M., Nemazee D., Jardine J.G., Safonova Y., Briney B., Rogers T.F., Wilson I.A., Baric R.S., Gralinski L.E., Burton D.R, & Andrabi R. (2023). Broadly neutralizing anti-S2 antibodies protect against all three human betacoronaviruses that cause deadly disease. Immunity, 56(3), 669-686.e7.

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Quantitative ELISA Assay for Proteins

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Solubilized CHO cell membrane protein (SMP), human insulin (Sigma-Aldrich cat.# I2643), single strand DNA (Sigma-Aldrich cat.# D8899) were coated onto 96-well half-area high-binding plates (Corning cat.# 3690) at 5μg /ml in PBS overnight at 4°C. After washing with PBST, plates were blocked with 3% BSA for 2h at 37°C. Antibody samples were diluted at 50μg /ml in 1% BSA with 5-fold serial dilution and then added in plates to incubate for 1h at room temperature (7 (link)). The assay was performed as described in section “ELISA using peptides or recombinant proteins”.

Zhou P., Song G., He W.T., Beutler N., Tse L.V., Martinez D.R., Schäfer A., Anzanello F., Yong P., Peng L., Dueker K., Musharrafieh R., Callaghan S., Capozzola T., Yuan M., Liu H., Limbo O., Parren M., Garcia E., Rawlings S.A., Smith D.M., Nemazee D., Jardine J.G., Wilson I.A., Safonova Y., Rogers T.F., Baric R.S., Gralinski L.E., Burton D.R, & Andrabi R. (2022). Broadly neutralizing anti-S2 antibodies protect against all three human betacoronaviruses that cause severe disease. bioRxiv.

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