Soluble cd14 scd14

Manufactured by R&D Systems

Sourced in Netherlands

Soluble CD14 (sCD14) is a protein that is released from the surface of cells and can be detected in the bloodstream. It functions as a co-receptor for the detection of bacterial cell wall components, such as lipopolysaccharide (LPS). sCD14 plays a role in the innate immune response.

Automatically generated - may contain errors

Lab products found in correlation

Endotoxin core antibodies igm, by Hycult Biotech (2 mentions) Limulus amebocyte lysate assay, by Hycult Biotech (2 mentions) Magnetic 33 plex assay, by R&D Systems (2 mentions) Ifabp, by MyBioSource (2 mentions) Lipopolysaccharide lps binding protein lbp, by Hycult Biotech (1 mentions) Salmonella typhimurium, by InvivoGen (1 mentions) Endocab, by Hycult Biotech (1 mentions) Fabp2, by R&D Systems (1 mentions) D dimer, by Diagnostica Stago (1 mentions) Interleukin 6 (il 6), by Mesoscale (1 mentions) High sensitivity il 6, by R&D Systems (1 mentions) Lp pla2, by R&D Systems (1 mentions) Hemosil d dimer hs 500 kit, by Werfen (1 mentions) Acl top, by Werfen (1 mentions) Mcp 1, by R&D Systems (1 mentions) Oxldl, by Mercodia (1 mentions)

Close spelling variants of this product

Soluble CD14 SCD14

5 protocols using soluble cd14 scd14

Serological Markers of Celiac Disease

Cited 3 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Established serological markers of coeliac disease, including IgA antibody to TG2 and IgG and IgA antibodies to deamidated gliadin, were measured as previously described.24 (link)
25 (link)
Serum IgG, IgA and IgM antibodies to native gliadin were measured separately by ELISA as previously described,24 (link)
26 (link) with the following modification: the secondary antibodies were horseradish peroxidase (HRP)-conjugated anti-human IgG (GE Healthcare), IgA (MP Biomedicals) or IgM (MP Biomedicals). Serum IgG, IgA and IgM antibodies to bacterial flagellin were measured separately using a similar protocol for detecting antibodies to gliadin, with the following modification: plates were coated with a 2 μg/mL solution of highly purified flagellin from Salmonella typhimurium (InvivoGen).
Levels of serum IgG, IgA and IgM endotoxin-core antibodies (EndoCAb) (Hycult Biotech), lipopolysaccharide (LPS)-binding protein (LBP) (Hycult Biotech), soluble CD14 (sCD14) (R&D Systems) and fatty acid-binding protein 2 (FABP2) (R&D Systems) were determined by ELISA, according to the manufacturers' protocols.

Uhde M., Ajamian M., Caio G., De Giorgio R., Indart A., Green P.H., Verna E.C., Volta U, & Alaedini A. (2016). Intestinal cell damage and systemic immune activation in individuals reporting sensitivity to wheat in the absence of coeliac disease. Gut, 65(12), 1930-1937.

+ Open protocol

+ Expand

Biomarker Testing in HIV Cohort

Check if the same lab product or an alternative is used in the 5 most similar protocols

At each study visit, blood was collected into EDTA tubes and tested for CD4⁺ cell count (Pima, Abbott Laboratories, Lake Bluff, Illinois, USA) and HIV-1 RNA viral load [Cobas Taqman platform in Uganda (Applied Biosciences, Beverley Hills, California, USA) and the CAP/CTM HIV-1 v2 assay (Roche Laboratories, Basel, Switzerland) in South Africa]. Additional blood was centrifuged for plasma separation, and frozen at -80^oC. Due to resource constraints that prevented us from testing the entire cohort, only nonpregnant individuals underwent additional testing of cryopreserved plasma for interleukin (IL-6; MesoScale Discovery, Rockville, Maryland, USA); soluble CD14 (sCD14; R&D Systems, Minneapolis, Minnesota, USA); and D-dimer (Diagnostica Stago, Parsippany, New York, USA). Biomarker assays were performed at the Laboratory for Clinical Biochemistry Research at the University of Vermont.

Siedner M.J., Bwana M.B., Asiimwe S., Musinguzi N., Castillo-Mancilla J., Amanyire G., Tracy R.P., Bangsberg D.R., Orrell C, & Haberer J.E. (2019). Inflammatory biomarkers prior to antiretroviral therapy as prognostic markers of 12-month mortality in South Africa and Uganda. AIDS (London, England), 33(13), 2043-2048.

+ Open protocol

+ Expand

Quantifying Endotoxin-Related Immune Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Levels of Endotoxin-core antibodies IgM (Hycult Biotech, Uden, Netherlands), soluble CD14 (sCD14) (R&D Systems, Minneapolis, MN, United States), and internal fatty acid-binding protein (iFABP) (MyBioSource, San Diego, CA, United States) in plasma samples were measured using an enzyme-immunoassay technique (ELISA) following the manufacturer’s protocol. Plasma levels of free bacterial endotoxin were measured using a chromogenic Limulus amebocyte lysate assay (Hycult Biotech, Uden, Netherlands) following the manufacturer’s protocol. Plasma levels of circulating immune factors were measured using a Magnetic 33-plex assay (R&D Systems, Minneapolis, MN, United States) and a MAGPIX instrument (Luminex, Austin, TX, United States). Bubble plots were generated using ggplot2 R package with concentration (pg/ml).

Rhoades N.S., Hendrickson S.M., Prongay K., Haertel A., Gill L., Edwards R.A., Garzel L., Slifka M.K, & Messaoudi I. (2021). Growth faltering regardless of chronic diarrhea is associated with mucosal immune dysfunction and microbial dysbiosis in the gut lumen. Mucosal Immunology, 14(5), 1113-1126.

+ Open protocol

+ Expand

Biomarker Quantification in Fasting Plasma

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Participants’ fasting, cryopreserved plasma samples were shipped to Temple University (Philadelphia, PA) for centralized quantification of levels of immune/inflammatory biomarkers using enzyme-linked immunosorbent assay kits: soluble CD14 (sCD14; R&D), soluble CD163 (sCD163; IQ Products), MCP-1 (R&D), high-sensitivity IL-6 (R&D), Lp-PLA2 (R&D), and oxLDL (Mercodia) [7 (link)]. D-dimer was measured using the HemosIL D-dimer HS 500 kit on the ACL TOP (Werfen). Fasting, cryopreserved serum samples were shipped to Quest Diagnostics for quantification of high-sensitivity C-reactive protein (hs-CRP) by commercially available assays.

Zanni M.V., Foldyna B., McCallum S., Burdo T.H., Looby S.E., Fitch K.V., Fulda E.S., Autissier P., Bloomfield G.S., Malvestutto C.D., Fichtenbaum C.J., Overton E.T., Aberg J.A., Erlandson K.M., Campbell T.B., Ellsworth G.B., Sheth A.N., Taiwo B., Currier J.S., Hoffmann U., Lu M.T., Douglas P.S., Ribaudo H.J, & Grinspoon S.K. (2022). Sex Differences in Subclinical Atherosclerosis and Systemic Immune Activation/Inflammation Among People With Human Immunodeficiency Virus in the United States. Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America, 76(2), 323-334.

+ Open protocol

+ Expand

Quantifying Endotoxin-Related Immune Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!