The expression of mesenchymal markers (CD29, CD44, CD90 and CD105) and hematopoietic markers (CD34 and CD45) on BAL-and BM-MSCs was examined by flow cytometry (Khatri et al., 2010 (link); Khatri et al., 2015 (link)). BM-MSCs were included as controls. The following primary antibodies specific to pig or cross-reactive human antibodies were used: mouse anti-pig CD29 (IgG1), mouse anti-pig CD44 (IgG2b), mouse anti-pig CD90 (IgG1, VMRD), mouse anti-pig CD45 (IgM, VMRD), mouse anti-pig/human CD105 (IgG2a, GeneTex), and FITC Mouse Anti-human CD34 (IgG1, BD Biosciences). MSCs were incubated with predetermined dilution of these antibodies for 30 min at 4° C. After the incubation, cells were washed and stained with specific secondary antibodies. Cells were then acquired using a FACS Aria II (BD Biosciences) flow cytometer and analyzed using FlowJo (Tree Star, Ashland, OR, USA) software (Khatri et al., 2010 (link)).
Fitc mouse anti human cd34 igg1
Manufactured by BD
FITC Mouse Anti-human CD34 (IgG1, κ) is a fluorescently labeled monoclonal antibody that binds to the CD34 surface antigen on human cells. CD34 is a transmembrane glycoprotein that is expressed on hematopoietic stem and progenitor cells.
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Lab products found in correlation
2 protocols using fitc mouse anti human cd34 igg1
1
Characterization of Porcine MSCs by Flow Cytometry
2
Immunophenotyping of Porcine MSCs
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L-MSCs were examined for the expression of mesenchymal markers (CD29, CD44, CD90 and CD105), hematopoietic markers (CD34 and CD45), monocyte (CD14) B cell (CD79β) and swine leucocyte antigen (SLA) I and II by flow cytometry. BM-MSCs were included as positive controls for comparison. MSCs were stained with the following primary antibodies: mouse anti-pig CD29 (IgG1), mouse anti-pig CD90 (IgG1), mouse anti-pig CD44 (IgG2b, VMRD), mouse anti-pig CD45 (IgM, VMRD), mouse anti-pig/human CD105 (IgG2a, GeneTex), Mouse anti pig CD14 (IgG2b, Serotec), rat anti-pig CD79b (IgG1, Serotec), FITC Mouse Anti-Human CD34 (IgG1, BD Biosciences), SLA-I (IgG2a, VMRD), SLA-II (IgG2b, VMRD; IgG2b FITC, Serotec) for 20 min at 4 ° C. After washing, cells were stained with appropriate secondary antibodies. Cells were acquired using a C6 flow cytometer (BD Accuri Cytometers) and analyzed using CFlow® plus Software (Accuri) [27 (link)].
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