Vanillic acid was purchased from Yuanye Bio-Technology Co., Ltd. (Shanghai, China). The primers were supplied by Sangon Biotech (Shanghai, China). The enzyme-linked immunosorbent assay (ELISA) kits for IL-1β and IL-18 were supplied by Invitrogen (Life Technologies Corp. California, United States). Fetal bovine serum (FBS), bovine serum albumin (BSA), Dulbecco’s Modified Eagle’s Medium (DMEM), TRIzol, and 0.25% trypsin-ethylenediaminetetraacetic acid (trypsin-EDTA) were purchased from Gibco (Life Technologies Corp., California, United States). Cell Counting Kit-8 (CCK-8) was purchased from Dojindo (Kumamoto, Japan). Antibodies against NLRP3, ASC, caspase-1, CGRP, NGF, TrkA, and type I collagen were purchased from Abcam (Cambridge, United Kingdom). Monoidoacetate acid, type I collagenase, and dimethylsulfoxide (DMSO) were all obtained from Sigma (St Louis, USA). All other chemicals were of reagent grade. Goat anti-rabbit IgG H&L (HRP) and Picro Sirius Red Stain kit were also supplied by Abcam (Cambridge, United Kingdom).
Trypsin ethylenediaminetetraacetic acid trypsin edta
Manufactured by Thermo Fisher Scientific
Sourced in United States
Trypsin-EDTA is a solution used to detach adherent cells from cell culture surfaces. It contains the enzyme trypsin, which cleaves cell-cell and cell-matrix adhesion proteins, and EDTA, which chelates calcium and magnesium ions required for cell-cell interactions. This solution is commonly used in cell culture procedures to enable passaging and harvesting of adherent cells.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Thermo Fisher Scientific (4 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Cell counting kit 8 cck 8, by Dojindo Laboratories (3 mentions)
Trizol reagent, by Thermo Fisher Scientific (3 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (3 mentions)
Sybr green master mix, by Bio-Rad (3 mentions)
Ethidium bromide, by Thermo Fisher Scientific (2 mentions)
Acridine orange, by Thermo Fisher Scientific (2 mentions)
Cdna synthesis kit, by Thermo Fisher Scientific (2 mentions)
4 6 diamino 2 phenylindole dihydrochloride dapi, by Merck Group (2 mentions)
Coomassie brilliant blue, by Bio-Rad (2 mentions)
Non essential amino acids (neaa), by Thermo Fisher Scientific (2 mentions)
Goat anti rabbit igg h l hrp, by Abcam (1 mentions)
Collagenase type 1, by Merck Group (1 mentions)
Picro sirius red stain kit, by Abcam (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Vanillic acid, by Yuanye Bio-Technology (1 mentions)
Aggrecan, by Abcam (1 mentions)
Gapdh, by Boster Bio (1 mentions)
Bovine serum albumin (bsa), by Beyotime (1 mentions)
15 protocols using trypsin ethylenediaminetetraacetic acid trypsin edta
1
NLRP3 Inflammasome Activation in Osteoarthritis
2
Investigating Stevioside's Anti-Osteoarthritis Effects
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Stevioside (STE) (purity ≥98%) was obtained from MedChemExpress (Shanghai, China). Recombinant mouse IL-1β was acquired from Novoprotein (China). Safranin-O/Fast Green and collagenase-II were procured from Solarbio (Beijing, China). Antibodies of collagen II, aggrecan, Nrf2, ADAMTS-4, and Lamin B1 were purchased from Abcam (Cambridge, UK). GAPDH, p65, HO-1, IkBα, and iNOS were purchased from Boster Biological Technology (Wuhan, China). MMP-13 and COX-2 were obtained from Cell Signaling Technology (Danvers, MA, USA). Fetal bovine serum (FBS), Dulbecco's modified Eagles' medium (DMEM)/F12, and 0.25% trypsin-ethylenediaminetetraacetic acid (trypsin–EDTA) were acquired from Gibco (Grand Island, NY, USA). Bovine serum albumin (BSA) was procured from Beyotime Biotechnology (Shanghai, China). The second antibodies of Goat Anti-Mouse IgG, Goat Anti-Rabbit IgG, Alexa Fluor®594 and Alexa Fluor®488 labeled were purchased from Bioworld (OH, USA).
3
Interleukin-1β Signaling Pathway Analysis
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Interleukin-1β was obtained from PeproTech (Rocky Hill, NJ, United States). Roswell Park Memorial Institute (RPMI) 1640 medium was purchased from Thermo Fisher Scientific Inc. (Logan, UT, United States). Antibodies against p-ERK (extracellular signal-related kinase), p-p38 MAPK (mitogen-activated protein kinase), p-JNK (c-Jun N-terminal kinase), NF-κB (nuclear factor kappa B) (p65), p-p65, I kappa B alpha (IκBα), p-c-Jun, p-Akt (protein kinase B), lamin B, and actin were purchased from Cell Signaling Technology Inc. (Beverly, MD, United States). TRIzol reagent was obtained from Invitrogen (Carlsbad, CA, United States). QuantiTect Reverse Transcription kit was purchased from Qiagen (Valencia, CA, United States). SYBR Green Master Mix was purchased from Bio-Rad Laboratories (Hercules, CA, United States). 0.25% trypsin ethylenediaminetetraacetic acid (trypsin-EDTA) was purchased from Gibco (Life Technologies Co., Carlsbad, CA, United States). Fetal bovine serum (FBS) and cell lysates were obtained from Sigma-Aldrich (St. Louis, MO, United States). The secondary antibodies were procured from Santa Cruz Biotechnology (Santa Cruz, CA, United States). All other reagents were obtained from Sigma Chemical Co. unless otherwise stated.
4
Biomaterial Synthesis and Cellular Uptake
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GA (purity: 99%) was obtained from Feiyu Biological Co., Ltd (Jiangsu, China). Boron powder, purchased from Maclin (Shanghai, China), was stored in an argon glove box. Isopropanol (IPA) and ethanol (anhydrous grade) were obtained from Sinopharm Chemical Reagents Co., Ltd. (Shanghai, China). PEG-NH2 (MW = 2000) was provided by Xi'an Regal Biotech Co., Ltd. Ultrapure water (18.25 MΩ.cm, 25 °C) was used to prepare all solutions. Phosphate-buffered saline (PBS, pH 7.4), fetal bovine serum (FBS), Dulbecco's modified eagle medium (DMEM), and trypsin-ethylenediaminetetraacetic acid (Trypsin-EDTA) were obtained from Thermo Fisher Scientific Inc. (USA). Cell counting kit-8 (CCK8) was purchased from Dojindo Laboratories (Japan). Cy5.5-PEG-NH2 and FITC-PEG-NH2 were obtained from Ruixi Biological Co., Ltd. (Shanxi, China). Propidium iodide (PI) and calcein acetoxymethyl ester (calcein-AM) were obtained from Solarbio Science & Technology Co., Ltd. (China). Methyl-β-cyclodextrin (MβCD), chlorpromazine (CPZ), hypertonic sucrose, and 5-(N-ethyl- N-isopropyl)-amiloride (EIPA) were purchased from Sigma Aldrich (St. Louis, MO, USA). 4′,6-diamidino-2-phenylindole (DAPI), Lyso Tracker, ER Tracker, and Mito Tracker were obtained from Molecular Probes Inc. (OR, USA).
5
Biodegradable Hydrogel Scaffold Synthesis
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Polycaprolactone (PCL; MW 80,000), poly(ethylene glycol) diacrylate (PEG-DA 575; MW 575), poly(N-isopropylacrylamide) (pNIPAAm; MW 40,000), 2-hydroxy-2-methylpropiophenone (HOMPP), chloroform, N,N-dimethylformamide (DMF), 2,2,2-trifluoroethanol (TFE), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), dimethyl sulfoxide (DMSO), phosphate-buffered saline (PBS; pH 7.4), alginic acid, and calcium chloride were purchased from Sigma-Aldrich (Milwaukee, WI, USA). Dulbecco’s modified Eagle’s medium (DMEM) with 1.0 g/L glucose, Dulbecco’s modified phosphate-buffered saline (DPBS), fetal bovine serum (FBS), penicillin/streptomycin (P/S), trypsin/ethylenediaminetetraacetic acid (trypsin/EDTA), acetoxymethyl calcein (calcein-AM), and ethidium homodimer-1 (EthD-1) were purchased from Thermo Fisher Scientific (Waltham, MA, USA).
6
Stimuli-responsive Drug Delivery System
7
Nanoformulations for Leishmania Treatment
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Glyceryl monostearate (GMS), soya lecithin (SL), polyoxyethylene sorbitan monooleate (Tween 80), cellulose dialysis tube (Mol. Weight; 14 kDa), Polyethylene glycol 400 (PEG 400) were obtained from HiMedia Laboratories (India). Trehalose was procured from TCI Chemical, India. Amphotericin B, paromomycin, 4′, 6-diamidino-2-phenylindole (DAPI), fluorenylmethoxycarbonyl chloride (Fmoc-Cl) and fluorescein isothiocyanate isomer I (FITC) were procured from Sigma-Aldrich (U.S.A). Fetal bovine serum (FBS), RPMI 1,640, penicillin–streptomycin, phalloidin-rhodamine, trypsin-ethylene diamine tetra acetic acid (Trypsin–EDTA) and phosphate buffer saline (PBS; pH–7.4), were purchased from Invitrogen (USA). Glass bottom dishes from the ThermoFiscer Scientific (USA), Silicon wafers and Carbon-coated TEM grids from Beta Tech Equipment (India).
8
Microglia Cell Culture Reagents and Protocols
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Cell culture reagents including Hank's Balanced Saline Solution (HBSS), 10,000 U/ml Penicillin/10,000 μg/ml streptomycin (PS), fetal bovine serum (FBS), horse serum, Dulbecco's Modified Eagle Medium with Ham's F-12 supplement (DMEM/F12), and 0.25% (w/v) Trypsin-ethylenediaminetetraacetic acid (trypsin-EDTA) were from Gibco (Thermo-Fisher Scientific, Burlington, ON, Canada). Rabbit anti-Iba1 antibody was from Wako (Osaka, Japan; RRID:AB_839504 ). Reagents for fluorescence assays including 4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora-3a, 4a-Diaza-s-Indacene (BODIPY 493/503), To-Pro-3 iodide, and donkey anti-rabbit Alexa Fluor 488 antibodies (RRID:AB_2535792 ) were from Invitrogen (Thermo Fisher Scientific, Burlington, ON, Canada). Chemicals and reagents were from Millipore Sigma (St. Louis, MO, USA) including Escherichia coli O111:B4 lipopolysaccharide (LPS), poly-l -lysine (PLL), and all SCCA used in culture experiments. Interferon γ (IFNγ) was from Peprotech (Montreal, QC, Canada). Enzyme-linked immunosorbent assay (ELISA) kits were from R&D Systems (Toronto, ON, Canada). Phosphate buffered saline was prepared from tablets to pH 7.4 (Bioshop Canada, Burlington, ON, Canada).
9
Crickets for Osteoindifferentiation Assays
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Crickets were collected from Wonju Natural Ecology Park (Wonju, Republic of Korea). Laemmli buffer (5×) and protein ladder were purchased from Dyne Bio Inc., Seongnam, Republic of Korea. Coomassie Brilliant Blue and SYBR Green Master mix were supplied by Bio-Rad Laboratories, USA. Dulbecco’s Modified Eagle’s Medium (DMEM), 10% fetal bovine serum (FBS), Dulbecco’s phosphate-buffered saline (DPBS), and antibiotics were purchased from Welgene Inc., Republic of Korea. Trypsin-ethylene diamine tetra acetic acid (Trypsin-EDTA) was provided by Gibco, USA. Osteo-induction media, 4,6-diamino-2-phenylindole dihydrochloride (DAPI), and alizarin red (ARS) staining kit were acquired from Sigma-Aldrich, USA. WST-1 dye and Alexa Fluor conjugated monoclonal antibodies were purchased from DoGenBio Co., Ltd., Republic of Korea, and Santa Cruz Biotechnology, USA, respectively. TRIzol® reagent, Acridine orange, and Ethidium bromide stains were purchased from Invitrogen, Thermo Fisher Scientific, USA. The cDNA synthesis kit was obtained from Invitrogen, Gaithersburg. The gene primers were supplied by BIONEER®Inc., Daejeon, Republic of Korea.
10
Adipogenic Differentiation Protocol
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The lipid staining dye Oil Red O (ORO, catalog: O0625), thiazolyl blue tetrazolium bromide (MTT), cell differentiation reagents 3-isobutyl-1-methylxanthine (IBMX, catalog: I7018), dexamethasone (DM, catalog: D8893), and insulin (catalog: I5500) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Dulbecco’s Modified Eagle’s Medium (DMEM, catalog: 12430-054) and supplements including bovine serum (BS, catalog: 26170-043), fetal bovine serum (FBS, catalog: 16000-044), penicillin/streptomycin (P/S, catalog: 15140-122), and trypsin-ethylenediaminetetraacetic acid (Trypsin-EDTA, catalog: 15400-054) were purchased from GIBCO-BRL (Grand Island, NY, USA). Isopropanol (2-propanol, catalog: 5035-44) was purchased from Daejung Chemicals & Materials (Siheung-si, Korea). Formalin (catalog: 6936050380) for cell fixation was purchased from Junsei (Tyoko, Japan). Primary antibodies against adipogenesis-related proteins, including PPAR-γ (catalog: #2443), C/EBP-α (catalog: #2295) and FABP4 (catalog: #2120), and against thermogenic proteins such as PGC-1α (catalog: #2178) and p-HSL (catalog: #4139), were purchased from Cell Signaling Technology (Bedford, MA, USA). Secondary antibody, anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH, catalog: sc-66163), and anti-SREBP-1 (catalog: sc-365513) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
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