Lysing matrix b tubes

Manufactured by Roche

Sourced in Germany, United States

Lysing Matrix B tubes are a product designed for sample preparation in laboratory settings. These tubes contain a matrix that facilitates the disruption and lysis of various types of cells, tissues, or microorganisms, allowing for the release and extraction of their contents for further analysis.

Automatically generated - may contain errors

Lab products found in correlation

Protease inhibitor cocktail, by Merck Group (1 mentions) Phosstop phosphatase inhibitor cocktail, by Roche (1 mentions) Screw cap micro tube, by Sarstedt (1 mentions) Direct detect, by Merck Group (1 mentions) Protease inhibitor cocktail, by Roche (1 mentions) Phosstop, by Roche (1 mentions) Magna lyser, by Roche (1 mentions)

2 protocols using lysing matrix b tubes

Protein Extraction from Cell Pellets

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The heat-inactivated cell pellets were resuspended in lysis buffer containing 2% SDS, 10 mM Tris-HCl (pH 7.5), 1 tablet per 50 mL EDTA-free Protease Inhibitor Cocktail (Sigma-Aldrich, Cleveland, US) and 1 tablet per 10 mL PhosSTOP Phosphatase Inhibitor Cocktail (Roche). The samples were subsequently transferred into Lysing Matrix B tubes (Roche) and disrupted mechanically by bead beating using MagNa Lyser (Roche Diognostics, GmbH, Mannheim, Germany) for 90 s, speed 6.0. The lysis procedure followed by 1 min cooling on ice was repeated six times. The lysate was clarified by centrifugation (15,000 × g for 15 min) at 21°C, and the supernatant containing the whole cell lysate proteins was transferred in to new 2 mL screw cap micro tubes (Sarstedt, Nümbrecht, Germany).

Yimer S.A., Birhanu A.G., Kalayou S., Riaz T., Zegeye E.D., Beyene G.T., Holm-Hansen C., Norheim G., Abebe M., Aseffa A, & Tønjum T. (2017). Comparative Proteomic Analysis of Mycobacterium tuberculosis Lineage 7 and Lineage 4 Strains Reveals Differentially Abundant Proteins Linked to Slow Growth and Virulence. Frontiers in Microbiology, 8, 795.

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Whole Cell Protein Extraction Protocol

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The inactivated cell pellets in lysis buffer containing 2% SDS, 10 mM Tris-HCl (pH 7.5), 10 mM DTT, EDTA free protease inhibitor cocktail (Roche), and PhosStop (Roche) were transferred into Lysing Matrix B tubes (Roche, US) and disrupted mechanically by bead beating with a MagNa Lyser for 90 s, speed 6.0 (Roche, US). The lysis procedure was repeated six times with 1 min cooling on ice between each bead beating. The lysate was clarified by centrifugation (15 000g for 15 min) at 21 °C, and the supernatant containing the whole cell lysate proteins was transferred to new 2 mL screw capped microtubes (Sarstedt, Germany). Protein concentration was measured with a Direct Detect infrared spectrometer (DirectDetect, Millipore).

Birhanu A.G., Yimer S.A., Holm-Hansen C., Norheim G., Aseffa A., Abebe M, & Tønjum T. (2017). Nε- and O-Acetylation in Mycobacterium tuberculosis Lineage 7 and Lineage 4 Strains: Proteins Involved in Bioenergetics, Virulence, and Antimicrobial Resistance Are Acetylated. Journal of proteome research, 16(11), 4045-4059.

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