HCT116, HeLa, MRC5, HFL-1, DU145, PC3, H1299 and T24 cell lines were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). SQ2OB cells were provided by Dr Ralph Weichselbaum (University of Chicago, Chicago, IL, USA). T24 cells were maintained in RPMI-1640, MRC5 cells in MEM and HFL-1 cells in DMEM/F-12 Ham’s. The HAP1 cell lines were purchased from Horizon Discovery (Cambridge, UK) and maintained in IMDM medium. HAP1 is a near-haploid human cell line derived from chronic myelogenous leukaemia cell line. HAP1 cells are adherent with fibroblast-like morphology. TOPK knockout was achieved via Crispr/Cas9 editing to contain a 4 bp deletion in the coding exon of TOPK. HUVEC and HMEC_1 cells were maintained in EBM-Plus (Lonza, Walkersville, MD, USA) medium. All other cells were maintained in DMEM. All cells were maintained with 10% FBS and all medium was purchased from Sigma (St Louis, MI, USA). All cell lines were authenticated by LGC standards (ATCC) by short tandem repeat profiling and tested for mycoplasma using MycoAlert (Lonza).
Ebm plus
Manufactured by Lonza
Sourced in United States
The EBM-Plus is a laboratory equipment product manufactured by Lonza. It is designed to facilitate the efficient processing and handling of biological samples. The core function of the EBM-Plus is to provide a reliable and consistent method for the extraction, purification, and preparation of various biomolecules, such as nucleic acids, proteins, and other cellular components, for further analysis and research applications.
Automatically generated - may contain errors
Lab products found in correlation
Hap1 cell line, by Horizon Discovery (1 mentions)
Hct116, by American Type Culture Collection (1 mentions)
Du145, by American Type Culture Collection (1 mentions)
H1299, by American Type Culture Collection (1 mentions)
Mycoalert, by Lonza (1 mentions)
Hfl 1, by American Type Culture Collection (1 mentions)
Mrc 5, by American Type Culture Collection (1 mentions)
3 protocols using ebm plus
1
Cell line characterization and TOPK knockout
2
Isolation and Culture of HUVECs
3
Isolation and Culture of HUVECs
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Human umbilical vein endothelial cells (HUVECs) were obtained as previously described,31 (link) with minor modifications. Briefly, umbilical cord veins were washed with phosphate-buffered saline (PBS) and subjected to collagenase digestion. The resultant HUVECs were seeded onto 96 well culture plates and maintained in endothelial basal medium EBM-Plus (Lonza, Walkersville, MD, USA). The cells used in this study were from second or third passage.
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