H1299 cells were transfected with scramble DNA, NS-siRNA or NPM-siRNA. 48 hours after transfection, cells were fixed in 4% PFA for 25 minutes, followed by treatment in 0.3% Triton X-100 for 20 minutes. The fixed cells were blocked with 5% BSA for 30 minutes, the cells were incubated with a mouse anti-ARF antibody (Cell signaling, CA) and a rabbit anti-NS antibody (Santa Cruz) at 4°C overnight. FITC-conjutated anti-mouse and Rhodamine-conjugated anti-rabbit IgG (Vector Laboratories, Burlingame, CA) were used for ARF and NS detection, and DAPI for nuclear staining. The cellular localization of NS/ARF was examined under a fluorescence microscope and double-negative cells were counted. At least six randomly chosen fields of view were examined with a minimum number of 800 cells scored for each condition.
Rabbit anti ns antibody
Manufactured by Santa Cruz Biotechnology
The Rabbit anti-NS antibody is a primary antibody that specifically binds to the NS antigen. It is a reliable tool for the detection and analysis of the NS protein in various research applications.
Automatically generated - may contain errors
2 protocols using rabbit anti ns antibody
1
Characterization of ARF and NS in H1299 Cells
2
Characterization of ARF and NS in H1299 Cells
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!