Carbopol 934

Tetrahydrocurcumin was kindly provided as a gift sample by Sanat Pharmaceuticals Ltd. (New Delhi, India). Carbopol^® 934, disodium hydrogen phosphate, sodium dihydrogen phosphate and Tween 80 were purchased from Central Drug House (P) Ltd. (New Delhi, India). Compritol^® 888ATO and Phospholipon 90 G were received as a gift sample from Panacea Biotec (Lalru, Punjab, India) and Gattefosse SAS, France, respectively. Chloroform and diethyl ether were purchased from Sisco Research Laboratories (Mumbai, India). Ethylene diamine tetra acetic acid and triethanolamine were provided by SD Fine Chemicals (Mumbai, India). Formalin and hydrochloric acid were supplied by Qualigens Fine Chemicals (Mumbai, India). Ellman reagent and 2,4-dinitro-chlorobenzene were obtained from Himedia Laboratories Ltd. (Mumbai, India). Methanol and polyethylene glycol were obtained from Fisher Scientific (Mumbai, India). 5,6-Carboxyfluoroscein was purchased from Sigma-Aldrich, Co., Burlington, MA, USA. The RayBio^® Mouse IL-6 ELISA Kit was provided by Ray Biotech, Inc., Norcross, GA, USA.

Simvastatin and poloxamer 188 and 407 were a kind gift sample from Sun Pharma, India. Lipid and cosurfactant were obtained from Gattefosse, France ex gratia. Formalin, hydrochloric acid, tris-hydrochloric acid, and methyl paraben were purchased from S.D fine chemicals Ltd., Mumbai, India. Bovine serum albumin, carbopol 934, disodium hydrogen phosphate, sodium hydrogen phosphate, thiobarbituric acid and tri-chloro acetic acid were purchased from central drug house Pvt. Ltd., Delhi, India. Chloroform, polyethylene glycol 400, potassium dihydrogen phosphate, and methanol were purchased from Fisher Scientific Pvt. Ltd., Mumbai, India. Hydrogen peroxide, sodium potassium tartarate, and sodium chloride were purchased from LobaChemie Pvt. Ltd., Mumbai, India. Copper sulphate and sodium hydroxide were purchased from Qualigens Fine Chemicals, Mumbai, India. Diethyl ether was purchased from Sisco Research Laboratories, Mumbai, India. Dialysis membrane (cut off of 12,000–14,000 Da) was purchased from Himedia Laboratories Ltd., Mumbai, India.

LEF and bovine serum albumin were obtained from Sigma-Aldrich (Milan, Italy). Compritol^® 888 ATO and Phospholipon^® 90 G were procured as a kind gift from Panacea Biotech (Lalru, India) and Phospholipid GmbH (Cologne, Germany), respectively. Carbopol^® 934 was provided by Central Drug House (P) Ltd. (New Delhi, India). Tween 80 was obtained from M/s Molychem Industries (Mumbai, India). Fertilized chicken (Cobb 500) eggs were acquired from Neelkanth Farms (Kurukshetra, India). Other reagents employed in this investigation were of pharmaceutical grade.