Rosmarinic acid

Manufactured by Yuanye Bio-Technology

Sourced in China

Rosmarinic acid is a natural compound extracted from certain plants. It functions as a powerful antioxidant with potential beneficial properties.

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Lab products found in correlation

12 protocols using rosmarinic acid

BZXD Marker Component Analysis

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According to the Pharmacopoeia of the People's Republic of China (2020 edition), six main components in BZXD have been identified. The quality stability and repeatability of these six marker components were detected by UPLC-MS/MS analysis method. We purchased the standard reference materials of Rosmarinic acid, Paeoniflorin, Glycyrrhizic acid, Salvianolic acid B, p-Coumaric acid, and Ferulic acid from Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Qualitative analysis was carried out using an AB Sciex API 4000 LC/MS/MS system. Analytes were separated using a linear gradient program at a flow rate of 0.30 ml/min using a mobile phase consisting of 2 mmol/l ammonium acetate in water (A) and acetonitrile (B).
Precisely pipette 0.200 ml of thawed BZXD concentrate at 4 °C into a 2 ml brown PE tube, 0.6 ml mass spectrometry grade acetonitrile solution, and vortex for 5 min. Centrifuge at 13000r/min at 4 °C for 20 min. Take the supernatant and dilute it by 4 times, shake and mix, and filter through a 0.22-micron filter to be tested. The samples were detected and analyzed by negative ion electrospray tandem mass spectrometry (Supplementary Figure S1).

He C., Wang Y., Wen Y., Li T., Hu E., Zeng S, & Xiong X. (2022). Quantitative proteomic analysis of Bi Zhong Xiao decoction against collagen-induced arthritis rats in the early and late stages. BMC Complementary Medicine and Therapies, 22, 186.

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Quantitative Analysis of Bioactive Compounds in AVP

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HPLC was performed using the Ultimate 3000 system (Thermo Fisher Scientific, Inc.). The reference substances neo chlorogenic acid, chlorogenic acid, rutin, isoquercitrin, astragaloside and rosmarinic acid (20 mg of each substance; Shanghai Yuanye Bio-Technology Co., Ltd.) were weighed and added to methanol at a chromatographic level of 2 ml. The reference substances were dissolved by full oscillation and reference substance reserve solutions were obtained. The two mobile phase solvents were: Mobile phase A, 0.5% acetic acid water; mobile phase B, acetonitrile. The flow rate was 0.5 ml/min. Accucore™ C18 chromatography columns (particle size 2.6 µm; dimensions, 4.6×150 mm; Thermo Fisher Scientific, Inc.) were used. The injection volume was 10 µl and the column temperature was 30°C. The detection wavelength was 328 nm and the gradient conditions 0–30 min, 12–45% (phase B); 30–35 min, 45–100% (phase B); 35–40 min, 100% (phase B) were used to determine the components of the AVP.

Guo H., Kuang Z., Zhang J., Zhao X., Pu P, & Yan J. (2019). The preventive effect of Apocynum venetum polyphenols on D-galactose-induced oxidative stress in mice. Experimental and Therapeutic Medicine, 19(1), 557-568.

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Standardized Analysis of Traditional Chinese Medicine

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Twenty-four authentic standards of adenosine (12), caffeic acid (17b), danshensu (17c), protocatechuic aldehyde (21), trans-5-caffeoylquinic acid (23b), ferulic acid (30), lithospermic acid (45b), salvianolic acid B (48a), baicalin (50a), ononin (50b), salvianolic acid A (61a), salvianolic acid C (70), astragaloside IV (77b), astragaloside III (79b), astragaloside II (83d), farrerol (84b), isoastragaloside II (85b), wogonin (86a), astragaloside I (92b), cyclosieversigenin (97b), dihydrotanshinone I (102a), tanshinone I (112), levistolide A (121) and tanshinone IIA (123) were all purchased from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). Five reference standards of calycosin7-O-β-d-glucoside (28b), rosmarinic acid (44), calycosin (62a), formononetin (80) and cryptotanshinone (130) were obtained from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). The structures of these reference standards (purity > 98%) are shown in Fig. 2. They were stored at 4 °C before use.
Acetonitrile, formic acid and water of LC-MS grade were purchased from Fisher Scientific Company (USA). Methanol of analytical grade was obtained from Runjie Tech Co. (Shanghai, China).
DZT and its five ingredients, i.e., LC, SM, PA, AM, and HN were provided by Tonghua Baishan Pharmaceutical Co., Ltd. (Jilin, China).

Cheng T., Ye J., Li H., Dong H., Xie N., Mi N., Zhang Z., Zou J., Jin H, & Zhang W. (2019). Hybrid multidimensional data acquisition and data processing strategy for comprehensive characterization of known, unknown and isomeric compounds from the compound Dan Zhi Tablet by UPLC-TWIMS-QTOFMS. RSC Advances, 9(16), 8714-8727.

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Comprehensive Analysis of Medicinal Compounds

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YXBCs were provided by Liaoning Good Nurse Pharmaceutical (Group) Co., Ltd. (Liaoning, China). Acetonitrile and methanol (HPLC grade) were purchased from Sigma-Aldrich Crop. (St. Louis, MO, USA). Formic acid (HPLC grade) was obtained from Shanghai Aladdin Bio-Chem Technology Co., Ltd. (Shanghai, China). The used water was purified by a Milli-Q water purification system (Millipore, Billerica, MA, USA). As reference compounds, danshensu, chlorogenic acid, ferulic acid, isochlorogenic acid B, rosmarinic acid, salvianolic acid B, salvianolic acid A, dihydrotanshinone Ⅰ, tanshinone IIA, and 3-acetyl-11-keto-β-boswellic acid (AKBA) were supplied by the Shanghai yuanye Bio-Technology Co., Ltd. (Shanghai, China), whose purities were all above 98%. Cryptotanshinone (>98%) was obtained from National Institutes for Food and Drug Control (Beijing, China).

Zhang X., Dong X., Zhang R., Zhou S., Wang W., Yang Y., Wang Y., Yu H., Ma J, & Chai X. (2024). Compounds identification and mechanism prediction of YuXueBi capsule in the treatment of arthritis by integrating UPLC/IM-QTOF-MS and network pharmacology. Heliyon, 10(7), e28736.

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Analytical Techniques for Neuroinflammation

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2,3,5-Triphenyltetrazolium chloride (TTC) and 5-bromo-2′-deoxyuridine (BrdU) were purchased from Sigma Chemical Co. Tanshinol, ferulic acid (FA), baicalin, protocatechuic acid, rosmarinic acid, salvianolic acid B, hydroxysafflor yellow A and 9′-methyl lithospermate B were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Rabbit monoclonal Bax antibody (#14796s, 1:1000), rabbit monoclonal IL-1β antibody (#12426s, 1:1000), rabbit monoclonal IL-6 antibody (#12912p, 1:1000) were purchased from Cell Signal Technology. Mouse monoclonal TNF antibody (#60291-1-Ig, 1:500), rabbit polyclonal BDNF antibody (#25699-1-AP, 1:200), and mouse β-actin antibody (#60008-1-Ig, 1:4000) were purchased from Proteintech Group, Inc., and rabbit polyclonal Bcl-2 antibody (#bs-0032R, 1:100) was purchased from Bioss (Beijing).

Zhang X., Zheng W., Wang T., Ren P., Wang F., Ma X., Wang J, & Huang X. (2017). Danshen-Chuanxiong-Honghua Ameliorates Cerebral Impairment and Improves Spatial Cognitive Deficits after Transient Focal Ischemia and Identification of Active Compounds. Frontiers in Pharmacology, 8, 452.

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HPLC Analysis of Medicinal Compounds in Salvia miltiorrhiza

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HPLC-grade acetonitrile was purchased from Merck Co., Inc. (Darmstadt, Germany). HPLC-grade phosphoric acid and analytical-grade methanol were purchased from Tianjin Komiou Chemical Reagent Co., Ltd. (Tianjin, China). Six reference compounds (rosmarinic acid, salvianolic acid B, dihydrotanshinone I, cryptotanshinone, tanshinone I and tanshinone IIA, HPLC ≥ 98%) were purchased from Shanghai Yuan ye Bio-Technology Co., Ltd. (Shanghai, China). Ultrapure water was generated with a You pu Ultrapure Water System (Chengdu, China).
The fresh roots and rhizomes of one-year-old S. miltiorrhiza plants were harvested in 2020 in Tongchuan (Tongchuan, China) in April, the best time of year for the harvest of the medicinal parts of S. miltiorrhiza, and were identified by Professor Zongsuo Liang (Zhejiang Sci-tech University). After removing soil and impurities, the samples were divided into 16 batches according to their weight, size and thickness.

Zhang L., Zhang X., Begum N., Xia P., Liu J, & Liang Z. (2022). Effects of Different Processing Methods Based on Different Drying Conditions on the Active Ingredients of Salvia miltiorrhiza Bunge. Molecules, 27(15), 4860.

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Quantification of Bioactive Compounds

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Chemical standards, including danshensu, caffeic acid, rosmarinic acid, cryptotanshinone, sugiol, carnosic acid, tanshinone IIA, and miltirone, were purchased from Shanghai Yuanye Biotechnology Corporation Ltd. (Shanghai, China). The other chemicals and reagents were purchased as follows: acetonitrile and methanol (HPLC grade), Merck (Darmstadt, Germany); warfarin, Sigma-Aldrich (Madrid, Spain); chloroform, Sinopharm Chemical Reagent (Shanghai, China); leucine encephalin, Waters (Milfor, Massachusetts, USA); pure distilled water, Watsons water (Hong Kong, China); and formic acid (HPLC grade), Fisher Scientific (Fair Lawn, New Jersey, USA).

Hu J., Wang F., Liang F., Wu Z., Jiang R., Li J., Chen J., Qiu S., Wang J., Zhang Y., Li Q, & Chen W. (2022). Identification of Abietane-Type Diterpenoids and Phenolic Acids Biosynthesis Genes in Salvia apiana Jepson Through Full-Length Transcriptomic and Metabolomic Profiling. Frontiers in Plant Science, 13, 919025.

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Radiological Injury and Rosmarinic Acid in Rats

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This study was approved by the Committee on the Ethics of Animal Experiments of
Guangxi Medical University (permit no. 201711014; Nanning, China). A total of
240 male specific pathogen-free Sprague-Dawley rats (9 weeks old, weighing 230 ±
20 g) were purchased from the Laboratory Animal Center of Guangxi Medical
University. The rats were housed in suspended plastic cages at 22°C to 24°C and
50% to 60% humidity on a 12-hour light/dark cycle. Food and water were provided
ad libitum. The rats were randomly divided into 6 groups: the control (Ctrl)
group, the (IR alone) group, the IR + 30, 60, and 120 mg/kg RA (IR and 30, 60,
and 120 mg/kg RA, respectively) groups, and the IR + AMI (irradiation and
Amifostine) group. Rosmarinic acid (Shanghai Yuanye Biotechnology, Shanghai,
China) was compounded with saline and was intragastrically administered 7 days
before IR and until the rats were sacrificed. Amifostine (Sigma-Aldrich, St.
Louis, MO; 250 mg/kg body weight in 1 mL saline solution) was intraperitoneally
administered 30 minutes before IR, which is effective and well tolerated by rats
to prevent saliva-flow dysfunction according to the previous research.^{25 (link)} Eight rats from each group were randomly sacrificed at each time point
(the 3rd, 10th, 30th, 60th, and 120th day after IR).

Zhang T., Liu C., Ma S., Gao Y, & Wang R. (2020). Protective Effect and Mechanism of Action of Rosmarinic Acid on Radiation-Induced Parotid Gland Injury in Rats. Dose-Response, 18(1), 1559325820907782.

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HPLC and GC-MS Analysis of Herbal Compounds

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HPLC grade methanol (MeOH), acetonitrile (ACN) and formic acid were purchased from Fisher Scientific (Pittsburgh, PA, United States) Ultrapure water was prepared by Synergy water purification system (Millipore, Billerica, United States). The reserpine standards (HPLC grade) and GC grade derivatizing regent MSTFA (N-methyl-N-(trimethylsilyl)trifluoroacetamide), methoxyamine hydrochloride were purchased from Sigma-Aldrich (St. Louis, MO, USA). Chemical reagent n-hexane (GC grade) and Anhydrous pyridine (GC grade) were obtained from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Salicylic acid, luteolin, apigenin and rosmarinic acid standards were provided by Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Reference standards of luteolin-7-O-glucoside, scutellarin, luteolin-7-O-glucuronide, apigenin-7-glucoside and apigenin-7-O-glucuronide were purchased from Shanghai Standard Technology Co., Ltd. (Shanghai, China). The purities of all standards were determined to be higher than 98%. Other chemicals and reagents were analytical grade.

Chen J., Guo L., Yang G., Yang A., Zheng Y, & Wang L. (2022). Metabolomic profiling of developing perilla leaves reveals the best harvest time. Frontiers in Plant Science, 13, 989755.

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Comprehensive Chemical Profiling of YGYSG

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The production of YGYSG was undertaken in The First Clinical Medical College at Anhui University of Chinese Medicine. A Milli-Q water purification system (Millipore, Bedford, MA, USA) was employed to acquire deionized water. For mass spectrometry analysis, formic acid of LC-MS grade was purchased from Sigma-Aldrich (St. Louis, MO, USA). Acetonitrile and methanol were LC-MS grade and obtained from Merck (Darmstadt, Germany). Overall, 27 chemical standards, including chikusetsusaponin IVa, chikusetsusaponin IV, ginsenoside Ro, 25R-inokosterone, β-ecdysone, acacetin, cymaroside, quercetin, gallic acid, oleanolic acid, hyperoside, chlorogenic acid, astragalin, kaempferol, cryptotanshinone, tanshinone II_A, rosmarinic acid, calycosin-7-O-β-D-glucoside, salvianolic acid B, astragaloside IV, formononetin-7-O-β-D-glycoside, calycosin, formononetin, scopoletin, ferulic acid, rutin, and narcissoside, were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China), and their purity levels exceeded 98% as determined through high-performance liquid chromatography (HPLC) analysis. Figure 2 displays the chemical configurations of each reference standard.

Yang F., Zhang K., Dai X, & Jiang W. (2024). Preliminary Exploration of Potential Active Ingredients and Molecular Mechanisms of Yanggan Yishui Granules for Treating Hypertensive Nephropathy Using UPLC-Q-TOF/MS Coupled with Network Pharmacology and Molecular Docking Strategy. Journal of Analytical Methods in Chemistry, 2024, 7967999.

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