Single cell suspensions were generated from spleen and lymph nodes as designated. Cells were stained with the following extracellular antibodies: anti-CD4 (RM4–5), anti-CD8 (53–6.7), anti-CD44 (IM7), anti-CXCR3 (CXCR3–173), anti-CX3CR1 (SA011F11), anti-CD45.1 (A20), anti-CD45.2 (104), anti-KLRG1 (2F1), anti-CD27 (LG.7F9), anti-CD62L (MEL-14) and anti-CD127 (A7R34). Antibodies were purchased from eBioscience, BD bioscience, Biolegend, or Tonbo. LCMV Db GP33 tetramer was provided by the NIH Tetramer Facility. For intracellular staining, cells were fixed with the FoxP3 Fix/perm kit (eBioscience) and stained with the following antibodies: anti-Eomesodermin (Dan11mag), anti-T-bet (4B10). For IV labelling experiments, tissues were harvested 3 minutes post-antibody injection.
Anti eomesodermin
Manufactured by Thermo Fisher Scientific
Anti-Eomesodermin is a laboratory reagent that can be used to detect the presence of the Eomesodermin protein in biological samples. Eomesodermin is a transcription factor involved in cellular processes, and the Anti-Eomesodermin reagent can be used to measure its expression levels.
Automatically generated - may contain errors
Lab products found in correlation
Foxp3 fix perm kit, by Thermo Fisher Scientific (1 mentions)
Anti pd 1, by BioLegend (1 mentions)
Anti nkg2a, by R&D Systems (1 mentions)
Anti cd8, by BD (1 mentions)
Anti cd56, by BD (1 mentions)
Anti nkg2d, by BD (1 mentions)
Anti cd28, by Thermo Fisher Scientific (1 mentions)
Anti cd127, by Thermo Fisher Scientific (1 mentions)
Aqua dead cell stain kit, by Thermo Fisher Scientific (1 mentions)
Anti ctla 4, by BD (1 mentions)
Anti cd94, by BD (1 mentions)
Anti t bet, by BioLegend (1 mentions)
Anti tnf, by BD (1 mentions)
Anti cd3, by Thermo Fisher Scientific (1 mentions)
Anti ifn γ, by BioLegend (1 mentions)
2 protocols using anti eomesodermin
1
Comprehensive Immune Cell Profiling
2
Multiparametric Flow Cytometry of Immune Cells
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Fluorescent monoclonal antibodies (mAbs) were purchased as follows: anti-CD8, anti-CD56, anti-CD94, anti-NKG2D, anti-CTLA-4 and anti-TNF from BD Bioscience (San Jose, CA); anti-CD127, anti-CD3, anti-CD28 and anti-Eomesodermin (Eomes) from eBioscience (San Diego, CA); anti-PD-1, anti-Tbet and anti-IFNγ from BioLegend (San Diego, CA); anti-NKG2A from R&D Systems (Minneapolis, MN); anti-Vδ1 TCR (clone REA173) from Miltenyi Biotec (San Diego, CA); anti-Vδ2 TCR (clone B6), anti-Vγ9 TCR (clone B3) and pan-γδ TCR (clone B1) from BioLegend (San Diego, CA). Dead cells were excluded using Aqua dead cell stain kit (Life Technologies). The phycoerythrin- or allophycocyanin-labeled CD1d tetramers were kindly provided by the NIH Tetramer Facility at Emory University (Atlanta, GA).
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