Anti pimonidazole mouse antibody

TGFβ1 was a kind gift from Dr F.W. Ruscetti (Biological Response Modifiers Program, FRCF, NCI, Maryland, USA). All fluorophore-coupled reagents (Alexa546-phalloïdin, Alexa488 anti-mouse IgG) were purchased from Invitrogen (Carlsbad, CA, USA). HDAC6 inhibitors (tubacin and CAY10603), SMAD3 inhibitor SIS3 and TGFBR inhibitor Galunisertib (LY2157299) were from Selleckchem (Houston, TX, USA). Cell nucleus stain 4′,6′-diamidino-2-phenylindole (DAPI) was from Invitrogen (Carlsbad, CA, USA). Anti-SMAD2, -SMAD3 and phosphoSMAD2(Ser465/467) and all HRP-coupled secondary antibodies were from Cell Signaling Technology (Boston, MA, USA). Anti-phosphoSMAD3(Ser423/425) was from Abcam (Cambridge, MA, USA). Anti-tubulin and anti-acetylated tubulin were from Sigma Aldrich (Oakville, ON, CA). Anti-SARA and anti-EEA1 antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Rabbit TrueBlot was purchased from Rockland Immunochemicals (Limerick, PA, USA). Pimonidazole hydrochloride and anti-pimonidazole mouse antibody were purchased from Hypoxyprobe (Burlington, MA). ITGB2 blocking antibody was from Novus Biologicals (Centennial, CO, USA). Anti-DDK antibody was from Origene.

LPS (Escherichia coli serotype O55:B5, L2880) was obtained from Sigma-Aldrich (USA). F4/80 (cat no. 29414-1-AP) and myeloperoxidase (MPO, cat no. 22225-1-AP) antibodies were from Proteintech (Wuhan, China), and hypoxia-inducible factor-1α (HIF-1α) antibody (cat no. BF8002) was from Affinity Biologicals (Jiangsu, China). XO (cat no. sc-398548) antibody was from Santa Cruz (USA). The XO activity assay kit (cat no. KTB1070), goat anti-mouse IgG H&L (DyLight 649) (cat no. A23610), goat anti-mouse IgG H&L (DyLight 488) (cat no. A23210), and goat anti-mouse IgG HRP (cat no. A21010) were purchased from Abbkine (Wuhan, China). Pimonidazole HCl and anti-pimonidazole mouse antibody was purchased from Hypoxyprobe (USA). ELISA kits for tumor necrosis factor-α (TNF-α, cat no. BMS607-3), interleukin-6 (IL-6, cat no. KMC0061), and IL-1β (cat no. KMC0061) were from Thermo Fisher Scientific (USA). The fluorescein (FITC) TUNEL Cell Apoptosis Detection Kit was from Servicebio (cat no. G1501, Wuhan, China). Dihydroethidium (DHE) for probing superoxide radicals was purchased from Beyotime (cat no. S0063, Shanghai, China).