Igg horseradish peroxidase hrp

Manufactured by Santa Cruz Biotechnology

Sourced in United States

IgG-horseradish peroxidase (HRP) is a conjugate of immunoglobulin G (IgG) and the enzyme horseradish peroxidase (HRP). This conjugate is designed for use in various immunoassay techniques, where it serves as a detection reagent to facilitate the visualization and quantification of target analytes.

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2 protocols using igg horseradish peroxidase hrp

Comprehensive Signaling Pathway Analysis

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p-Akt (Ser473) (#4060), p-Akt (Thr308) (#13038), Akt (#4691), Cyclin D1 (#2978), p-p38 (Thr180/Tyr182) (#4511), p-ERK1/2 (Thr202/Tyr204) (#4370), ERK1/2 (#9102), p38 (#8690), Bcl-2 (#3498), K17 (#4543), p-IKKα/β (Ser176/180) (#2697), IKKβ (#8943), p-IκBα (Ser32) (#2859), p-p65 (Ser536) (#3033), p65 (#8242), p-p70S6K (#9234), p70S6K (#9202), HDAC1 (#5356), HDAC2 (#5113), HDAC3 (#3949), HDAC4 (#7628), HDAC6(#7558), H3 (#4499), p-SAPK/JNK (Thr183/Tyr185) (#4668), and SAPK/JNK (#9252) antibodies were purchased from Cell Signaling Technologies, USA. mTOR (# sc-1549), ICAM-1 (#sc-8439), β-Actin (#sc-47778), COX-2 (#sc-1745), PCNA (#sc-7907), Ki-67 (#sc-15402), p-STAT3 (#sc-8059), STAT3 (#sc-8019), anti-Goat (#Sc-2354), anti-mouse (#Sc-2061), and anti-mouse (#Sc-2030) IgG-horseradish peroxidase (HRP) antibodies were procured from Santa Cruz Biotechnology, USA.

Thatikonda S., Pooladanda V., Sigalapalli D.K, & Godugu C. (2020). Piperlongumine regulates epigenetic modulation and alleviates psoriasis-like skin inflammation via inhibition of hyperproliferation and inflammation. Cell Death & Disease, 11(1), 21.

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Western Blot Analysis of Protein Expression

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Cells were lysed in RIPA lysis buffer with proteinase inhibitor cocktail (Boster, Wuhan, China) and total proteins were extracted. Proteins were quantified using the BCA protein assay (Pierce, Rockford, IL, U.S.A.). Forty micrograms of proteins were separated by 10% SDS/PAGE, transferred on to PVDF membranes (Millipore, Billerica, MA, U.S.A.) and immune blotted with the respective primary antibodies at 4°C overnight. After washing three times, the membranes were incubated with HRP-linked secondary antibody at room temperature for 1 h. Protein bands were signaled using a chemiluminescence (ECL) reagent (Beyotime Biotechnology). The following antibodies were used in the present study with the respective concentration: p-glycoprotein (P-gp) (1:1000; Cell Signaling Technology, Danvers, MA, U.S.A.), Ki67 (1:200; Santa Cruz Biotechnology, Santa Cruz, CA), cleaved caspase-3 (1:1000; Cell Signaling Technology), β-catenin (1:400; Santa Cruz Biotechnology), c-Myc (1:1000; Cell Signaling Technology), cyclinD1 (1:800; Santa Cruz Biotechnology), β-actin (1:1000; Cell Signaling Technology), and IgG-horseradish peroxidase (HRP) (1:4000; Santa Cruz Biotechnology).

Kang Y., Zhang S., Cao W., Wan D, & Sun L. (2020). Knockdown of LncRNA CRNDE suppresses proliferation and P-glycoprotein-mediated multidrug resistance in acute myelocytic leukemia through the Wnt/β-catenin pathway. Bioscience Reports, 40(6), BSR20193450.

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