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Antibodies against β actin clone w16197a

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Antibodies against β-actin (clone W16197A) are affinity-purified immunoglobulins that specifically recognize the β-actin protein. These antibodies can be used for the detection and quantification of β-actin in various applications, such as Western blotting, immunohistochemistry, and immunocytochemistry.

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Lab products found in correlation

Krt10 clone de k10, by BioLegend (2 mentions) Antibody against ivl ma5 11803, by Thermo Fisher Scientific (2 mentions) Laemmli sample buffer, by Bio-Rad (2 mentions) Antibodies against il, by R&D Systems (1 mentions)

Close spelling variants of this product

β-actin (13 citations) β–actin (W16197A; (2 citations)

2 protocols using antibodies against β actin clone w16197a

1

Western Blot Analysis of Gene-Silenced NHPK Cells

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Scrambled- and specific gene-siRNA–silenced differentiated NHPK Cells were lysed in 2× Laemmli sample buffer (Bio-Rad), and proteins were run on Western blots. Antibodies against β-actin (clone W16197A) and KRT10 (clone DE-K10) were purchased from BioLegend (San Diego, CA); antibody against IVL (MA5–11803) was purchased from Thermo Fisher Scientific (Carlsbad, CA); and antibody against LOR was a kind gift from Dr. Dennis Roop (University of Colorado).31 (link) Antibodies against IL1β and IFNκ were purchased from R&D biosystems (Minneapolis, MN).
Bin L., Malley C., Taylor P., Boorgula M.P., Chavan S., Daya M., Mathias M., Shankar G., Rafaels N., Vergara C., Potee J., Campbell M., Hanifin J.M., Simpson E., Schneider L.C., Gallo R.L., Hata T., Paller A.S., De Benedetto A., Beck L.A., Ong P.Y., Guttman-Yassky E., Richers B., Baraghoshi D., Ruczinski I., Barnes K.C., Leung D.Y, & Mathias R.A. (2021). Whole genome sequencing identifies novel genetic mutations in patients with eczema herpeticum. Allergy, 76(8), 2510-2523.
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2

Western Blot Protein Analysis

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Cells were lysed in 2x Laemmli sample buffer (Bio-Rad) and proteins were run on western blots. Antibodies against β-actin (clone W16197A) and KRT10 (clone DE-K10) were purchased from BioLegend; antibody against IVL (MA5-11803) was purchased from Thermo Scientific. Rabbit polyclonal anti-IFNκ (ab168119) was purchased from Abcam (Cambridge, MA).
Li Y., Song Y., Zhu L., Wang X., Richers B., Leung D.Y, & Bin L. (2020). Interferon Kappa Is Important for Keratinocyte Host Defense against Herpes Simplex Virus-1. Journal of Immunology Research, 2020, 5084682.
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