Elecsys insulin kit

Manufactured by Roche

Sourced in United States, Germany

The Elecsys Insulin kit is a laboratory diagnostic test used to quantitatively measure insulin levels in human blood samples. The kit utilizes an electrochemiluminescence immunoassay (ECLIA) technology to perform the analysis.

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Lab products found in correlation

Gluc3 glucose hexokinase kit, by Roche (1 mentions) Cobas e601, by Roche (1 mentions) Cobas c501, by Roche (1 mentions) M6 hem 7001 e, by Omron (1 mentions) Cobas 6000 analyzer, by Hitachi (1 mentions) Cobas 411, by Roche (1 mentions)

5 protocols using elecsys insulin kit

Fasting Biomarkers for Insulin Resistance

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The fasting blood sugar (FBS) was measured in freshly collected samples by Cobas c 501 (Roche, USA) using Gluc3-Glucose hexokinase kit (Catalogue no: 04404483–190) from Roche, USA.
The fasting serum insulin level was measured freshly collected samples by fully automated electro immunoassay analyzer (Cobas e 601 from Roche, USA) using Elecsys Insulin kit (Catalogue no: 12017547–122, from Roche, USA).
The level of HbA1c was estimated in freshly collected samples by Bio-Rad D-10 haemoglobin system (Bio-Rad, USA) using D-10 Hemoglobin A1c kit (Catalogue no: 220–0101, from Bio-Rad, USA).
Homeostatic model assessment-insulin resistance (HOMA-IR) was calculated using FBS and fasting insulin level.

R. R., Uthaiah C.A., C. M. R., Madhunapantula S.V., Salimath P.V., K. P., M. S.K, & M. R. K. (2022). Comparative assessment of cognitive impairment and oxidative stress markers among vitamin D insufficient elderly patients with and without type 2 diabetes mellitus (T2DM). PLoS ONE, 17(6), e0269394.

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Metabolic and Anthropometric Profile Assessment

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The women attended hospital between 8:00 and 10:00 am after overnight fast. A blood sample was obtained for biochemical determinations and DNA extraction. On the same visit anthropometric characteristics were recorded and blood pressure was taken after sitting for at least 5 minutes.
Blood pressure was measured with an Omron M6 (HEM-7001-E) oscillometric blood pressure monitor (Omron Healthcare, Kyoto, Japan). Body mass index (BMI) was calculated as weight (kg) divided by height squared (m²).
The serum was separated and freshly used for most biochemical determinations, and kept frozen at −80°C for tests not performed on the same day. Insulin levels were measured with electrochemiluminescence immunoassay (ECLIA, Elecsys Insulin kit, Roche Cobas, Mannheim, Germany) in a Modular Analytics E170 analyzer. Levels of glucose, total calcium and phosphate, total alkaline phosphatase (ALP), triglycerides (TG), total cholesterol (TC), and high-density lipoprotein cholesterol (HDL) were measured using a spectrophotometer (Olympus 5400, Olympus, Melville, NY, USA).
Low-density lipoprotein cholesterol (LDL; mg/dl)) was calculated as TC − (HDL+TG/5),^[29] (link) and the HOMA_IR (homeostasis model assessment) insulin resistance index was estimated as fasting serum insulin in μIU/ml × (fasting serum glucose in mg/dl × 0.05551)/22.5.^[30] (link)

Pertusa C., Tarín J.J., Cano A, & García-Pérez M.A. (2020). Association of a single nucleotide polymorphism of RANK gene with blood pressure in Spanish women. Medicine, 99(40), e22436.

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Serum Insulin Concentration Determination

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The Department of Pathology and Laboratory Medicine at MEIH-UH determined the serum concentration of insulin via a sandwich ELISA principle using the Elecsys insulin kit (Roche, Basel, Switzerland) by using Roche/Hitachi Cobas^® 6000 analyzer.

Abou-Samra M., Venema K., Ayoub Moubareck C, & Karavetian M. (2022). The Association of Peptide Hormones with Glycemia, Dyslipidemia, and Obesity in Lebanese Individuals. Metabolites, 12(11), 1051.

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Metabolic Biomarkers Assessment

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Fasting blood samples were collected between 9 and 11 a.m. Plasma glucose, cholesterol, HDL cholesterol, and triglycerides were analysed using Vitro 4000 (Ortho Clinical Diagnostics). The concentration of LDL cholesterol was calculated. Insulin levels were measured using the Elecsys Insulin kit in combination with the Cobas 411 (Roche Diagnostics, Germany) equipment. The homeostasis model assessment-estimated insulin resistance (HOMA-IR) index was calculated according to the original formula [32 (link)].

Belva F., Bonduelle M., Provyn S., Painter R.C., Tournaye H., Roelants M, & De Schepper J. (2018). Metabolic Syndrome and Its Components in Young Adults Conceived by ICSI. International Journal of Endocrinology, 2018, 8170518.

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Lipid and Glucose Biomarker Analysis

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Concentrations of TC, TG, HDL-C, and glucose were analyzed with an automatic analyzer (Technicon RA 1000 Chemistry Analyzer) and a commercially available kit (Laborlab, São Paulo, Brazil). The LDL-C was calculated according to the Friedewald equation (18 (link)). The insulin was determined by chemiluminescence with commercial kits (Elecsys insulin kit, Roche Diagnostics GmbH, Indianapolis, IN, USA) and an automatic biochemical analyzer (ARCHITECT i2000 SR, Abbot Diagnostics, IL, USA). The HbA1c was verified by high pressure liquid chromatography high performance (HPLC).

Bonfante I.L., Chacon-Mikahil M.P., Brunelli D.T., Gáspari A.F., Duft R.G., Oliveira A.G., Araujo T.G., Saad M.J, & Cavaglieri C.R. (2017). Obese with higher FNDC5/Irisin levels have a better metabolic profile, lower lipopolysaccharide levels and type 2 diabetes risk. Archives of Endocrinology and Metabolism, 61(6), 524-533.

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