Capillary cell

Manufactured by Malvern Panalytical

Sourced in United Kingdom, Germany

Capillary cells are specialized lab equipment used to study the properties and behavior of materials in a small-scale environment. They enable the analysis of sample materials in a confined, controlled space, facilitating precise measurements and observations.

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Lab products found in correlation

Zetasizer nano z, by Malvern Panalytical (5 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions) Zetasizer nano zs pn3702, by Malvern Panalytical (2 mentions) Malvern zetasizer software 7, by Malvern Panalytical (1 mentions) Malvern zetasizer nano z, by Malvern Panalytical (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Sodium azide, by Merck Group (1 mentions) H 7650, by Hitachi (1 mentions) Zetasizer, by Malvern Panalytical (1 mentions)

Close spelling variants of this product

DT51070 folded capillary cells Plain folded capillary zeta cells DTS1070 capillary cells DTS1060 capillary cells DTS-1070 disposable folded capillary cells Folded Capillary Cell DTS1060 Disposable capillary zeta cell Capillary cell cuvette DTS1070 folded capillary zeta cell DTS1070 disposable capillary cell

11 protocols using capillary cell

Dynamic Light Scattering and Zeta Potential Analysis

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Hydrodynamic diameters and polydispersity were
determined by dynamic light scattering (DLS) experiments using a Malvern
Zetasizer ZS apparatus. We used a 633 nm He–Ne laser and backscattered
detection optic arrangement, i.e., detector placed at 7° with
respect to the incident light beam. All of the measurements were carried
out in 7 mM HEPES pH 7.4 buffer containing 35 mM KCl. ζ potential
measurements were determined from three independent experiments to
ensure reproducibility, using capillary cells (Malvern Instruments)
with a drive cell voltage of 30 V. For the ζ potential calculations,
the Smoluchowski approximation of the Henry equation was employed.

Giménez R.E., Piccinini E., Azzaroni O, & Rafti M. (2019). Lectin-Recognizable MOF Glyconanoparticles: Supramolecular Glycosylation of ZIF-8 Nanocrystals by Sugar-Based Surfactants. ACS Omega, 4(1), 842-848.

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Nanoparticle Characterization and Stability

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The hydrodynamic diameter (reported as size in the following) and the zeta potential of the NPs was determined by DLS and measuring the electrophoretic mobility, respectively, using a Malvern Zetasizer Nano ZS (Malvern, Herrenberg, Germany). For DLS and zeta potential measurements, particle stock solutions were diluted in DPBS (1:10) and the general-purpose mode with automatic measurement position was applied. For zeta potential measurements, particles were analyzed using capillary cells (Malvern, Herrenberg, Germany). To determine NP stability, NPs were incubated for 25 h at 37 °C in DPBS as well as Dulbecco’s modified Eagle’s medium (DMEM; Pan Biotech GmbH, Aidenbach, Germany) containing 10% (v/v) fetal bovine serum (FBS) and 0.01% (m/v) sodium azide (Merck KGaA, Darmstadt, Germany) for 24 h. At indicated time points, samples were taken, and the size was measured at 37 °C as described. Data were recorded using the Malvern Zetasizer software 7.11 (Malvern Instruments, Worcestershire, UK).

Mietzner R., Pawlak R., Tamm E.R., Goepferich A., Fuchshofer R, & Breunig M. (2021). Angiopoietin-1 Mimetic Nanoparticles for Restoring the Function of Endothelial Cells as Potential Therapeutic for Glaucoma. Pharmaceuticals, 15(1), 18.

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Dynamic Light Scattering of Nanoparticles

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The nanoparticles were dissolved (1 mg in 1 mL of distilled water) and analyzed using dynamic light scattering (DLS) on a ZetaSizer NanoZS (Malvern Instruments Ltd., Worcestershire, UK) particle size analyzer. Samples were diluted with distilled water before measurement in capillary cells (Malvern Instruments Ltd., Malvern, Worcestershire, UK). The temperature of the samples was maintained at 25 °C throughout the analyses.

Essa D., Kondiah P.P., Kumar P, & Choonara Y.E. (2023). Design of Chitosan-Coated, Quercetin-Loaded PLGA Nanoparticles for Enhanced PSMA-Specific Activity on LnCap Prostate Cancer Cells. Biomedicines, 11(4), 1201.

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Characterization of SP-A Interaction with Nanoparticles

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The interaction between SP-A and NPs was characterised by dynamic light scatter (DLS; HPPS and Zetasizer Nano ZS, Malvern Instruments, Worcestershire, UK) and zeta potential (ZP; Zetasizer Nano ZS, Malvern Instruments). This was initially performed in nano pure water as described previously (Kendall et al., 2013 (link)). The DLS and ZP analyses were also performed in TBS and serum-free (SF) RPMI-1640 medium without phenol red (Gibco, Life Technologies, UK). In these experiments, A-PS or U-PS particles were suspended in TBS with 5 mM calcium at a concentration of 12.5 cm²/mL. These particles were diluted with equal volumes of protein (SP-A or BSA) suspended in TBS + calcium at 50 µg/mL. The size and ZPs of these suspensions were measured immediately before (T-2) and after (T0) the addition of protein. The particle suspensions were then incubated at 37 °C for 48 min, the size and ZP were then measured before (T48) and after (T60) the addition of SF RPMI. This yielded a TBS/RPMI ratio of 2:3 and final concentrations of proteins and particles of 10 µg/mL and 2.5 cm²/mL, respectively. The size and ZP of the suspensions were measured again following incubation for a further two hours at 37 °C (T180). All measurements were conducted at 37 °C using reusable or disposable capillary cells (Malvern Instruments).

McKenzie Z., Kendall M., Mackay R.M., Whitwell H., Elgy C., Ding P., Mahajan S., Morgan C., Griffiths M., Clark H, & Madsen J. (2015). Surfactant protein A (SP-A) inhibits agglomeration and macrophage uptake of toxic amine modified nanoparticles. Nanotoxicology, 9(8), 952-962.

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Characterizing SP-A Interaction with NPs

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The interaction between SP-A and NPs was characterised by DLS (HPPS and Zetasizer Nano ZS, Malvern Instruments, UK) and ZP (Zetasizer Nano ZS, Malvern Instruments). This was initially performed in nano pure water as described previously (Kendall et al., 2013 (link)). The DLS and ZP analyses were also performed in Tris buffered saline (TBS) and serum free RPMI-1640 medium without phenol red (Gibco, Life Technologies, UK). In these experiments, A-PS or U-PS particles were suspended in TBS with 5 mM calcium at a concentration of 12.5 cm²/mL. These particles were diluted with equal volumes of protein (SP-A or BSA) suspended in TBS + calcium at 50 μg/mL. The size and zeta potentials of these suspensions were measured immediately before (T-2) and after (T0) the addition of protein. The particle suspensions were then incubated at 37°C for 48 minutes, the size and ZP were then measured before (T48) and after (T60) the addition of serum free (SF) RPMI. This yielded a TBS/RPMI ratio of 2:3 and final concentrations of proteins and particles of 10 μg/mL and 2.5 cm²/mL respectively. The size and ZP of the suspensions were measured again following incubation for a further 2 hours at 37°C (T180). All measurements were conducted at 37°C using reusable or disposable capillary cells (Malvern Instruments).

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Characterization of Silica Nanoparticles

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TEM (H-7650; Hitachi High-Technologies Corporation, Tokyo, Japan) was used to assess the size and shape of the silica nanoparticles. nSP30 and nSP30C nanoparticles were diluted to 0.25 mg mL⁻¹ in PBS or deionized water, and the mean particle diameter and zeta potential at 25 °C were measured by using a Zetasizer Nano ZS (Malvern Instruments, Worcestershire, UK). Specifically, the mean diameters and particle size distributions of the nanoparticles were measured by means of a dynamic light-scattering method, whereas zeta potentials were measured by laser Doppler electrophoresis; both types of measurements were performed by using capillary cells (Malvern Instruments). The pH of each particle suspension was measured by using an ISFET pH meter (Shindengen, Tokyo, Japan).

Hirai T., Yoshioka Y., Takahashi H., Ichihashi K.I., Udaka A., Mori T., Nishijima N., Yoshida T., Nagano K., Kamada H., Tsunoda S.I., Takagi T., Ishii K.J., Nabeshi H., Yoshikawa T., Higashisaka K, & Tsutsumi Y. (2015). Cutaneous exposure to agglomerates of silica nanoparticles and allergen results in IgE-biased immune response and increased sensitivity to anaphylaxis in mice. Particle and Fibre Toxicology, 12, 16.

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Evaluating Surface Charges of Alginate Particles

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To analyze the synthesized surface charges spherical (modified) ALG (50 µm in diameter, total surface area: 10 cm²) suspended in PBS were evaluated at 25 °C in a capillary cell (Malvern Instruments, Worcestershire, England) using the Zetasizer Nano-ZS PN3702 (Malvern Instruments, Worcestershire, England). Changes in zeta potentials due to protein adsorption were measured after 24 h incubation of spherical (modified) ALG suspended in DMEM/F12 supplemented with 10% FCS at 37 °C and subsequently washed with PBS before analyzing.

Schulz A., Katsen-Globa A., Huber E.J., Mueller S.C., Kreiner A., Pütz N., Gepp M.M., Fischer B., Stracke F., von Briesen H., Neubauer J.C, & Zimmermann H. (2018). Poly(amidoamine)-alginate hydrogels: directing the behavior of mesenchymal stem cells with charged hydrogel surfaces. Journal of Materials Science. Materials in Medicine, 29(7), 105.

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Zeta-Potential Characterization of Nanoparticles

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ζ-Potential measurements were obtained by laser-Doppler anemometry using a Zetasizer (Malvern Instruments, Malvern, UK). Measurements were carried out at 25°C in an aqueous solution using an effective voltage of 150 V. Briefly, 5 mg of each lyophilized NP formulation was weighed, placed in a flask, diluted with 50 mL of distilled water, and kept in a sonicator for 5 minutes. The aqueous dispersions of NPs were then introduced into a capillary cell (Malvern Instruments) for ζ-potential measurements. All formulations were analyzed in triplicate.

Marcianes P., Negro S., García-García L., Montejo C., Barcia E, & Fernández-Carballido A. (2017). Surface-modified gatifloxacin nanoparticles with potential for treating central nervous system tuberculosis. International Journal of Nanomedicine, 12, 1959-1968.

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Zeta Potential Analysis of Alginate Particles

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Zeta potentials of (modified) spherical ALG (Ø 50 μm, 10 cm² total surface area) suspended in PBS were measured at 25°C in a capillary cell (Malvern Instruments, Worcestershire, England) using the Zetasizer Nano‐ZS PN3702 (Malvern Instruments, Worcestershire, England). Surface charges after protein adsorption were analyzed after the incubation (24 h) of spherical ALG in DMEM/F‐12 supplemented with 10% FCS at 37°C and three washing steps with NaCl before analyzing.

Schulz A., Gepp M.M., Stracke F., von Briesen H., Neubauer J.C, & Zimmermann H. (2018). Tyramine‐conjugated alginate hydrogels as a platform for bioactive scaffolds. Journal of Biomedical Materials Research. Part a, 107(1), 114-121.

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Zeta Potential of Digesta Solution

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The zeta potential of digesta solution from each digestion phase was measured using a Zetasizer Nano ZS (Malvern, Herrenberg, Germany). The diluted sample was injected into a capillary cell (Malvern, Herrenberg, Germany), and was measured at 25 °C in the measurement chamber.

Zhu Y., Peng Y., Wen J, & Quek S.Y. (2021). A Comparison of Microfluidic-Jet Spray Drying, Two-Fluid Nozzle Spray Drying, and Freeze-Drying for Co-Encapsulating β-Carotene, Lutein, Zeaxanthin, and Fish Oil. Foods, 10(7), 1522.

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