A549 human pulmonary epithelial cell line was purchased from the American Type Culture Collection (Manassas, VA, USA). Enoxaparin was obtained from Aventis Pharma Ltd. (NSW, Australia). UFH was purchased from Hospira Pty. Ltd. (Victoria, Australia). Sodium chloride, tetrahydrofuran, sodium hydroxide, N-methyl-N-(trimethylsilyl) trifluroacetamide, methanol, acetic acid, Ham’s F12K medium, antibiotics (penicillin G and streptomycin), trypsin-ethylenediaminetetraacetic acid (EDTA), trypsin, thrombin, enzyme-linked immunosorbent assay (ELISA) kits for IL-6 and IL-8, trypan blue exclusion assay kit, lactate dehydrogenase (LDH) activity assay kit, and potassium phosphate monobasic and dibasic were purchased from Sigma-Aldrich (Castle Hill, NSW, Australia). Fetal bovine serum, IL-6 and IL-8 recombinant human proteins were obtained from Invitrogen (Grand Island, NY, USA). Deuterium oxide (D2O) was purchased from Cambridge Isotope Laboratories (Andover, MA, USA).
Trypan blue exclusion assay kit
Manufactured by Merck Group
Sourced in Australia
The Trypan blue exclusion assay kit is a laboratory tool used to determine the viability of cells. It is a simple and widely used method for differentiating live and dead cells. The kit provides a solution of trypan blue dye, which selectively stains dead cells, allowing them to be counted and distinguished from live, unstained cells under a microscope.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Thrombin, by Merck Group (1 mentions)
Deuterium oxide d2o, by Cambridge Isotopes (1 mentions)
Ham s f 12k medium, by Merck Group (1 mentions)
Potassium phosphate monobasic and dibasic, by Merck Group (1 mentions)
N methyl n trimethylsilyl trifluroacetamide, by Merck Group (1 mentions)
Lactate dehydrogenase ldh activity assay kit, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Trypsin, by Merck Group (1 mentions)
Acetic acid, by Merck Group (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
Tetrahydrofuran, by Merck Group (1 mentions)
A549 human pulmonary epithelial cell line, by American Type Culture Collection (1 mentions)
2 protocols using trypan blue exclusion assay kit
1
Evaluating Enoxaparin and UFH Effects on A549 Cells
2
Trypan Blue Cell Proliferation Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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To assess the effect of various transfections or retrovirus-based stable gene expression or treatments on cell proliferation, the cells were trypsinized and number of viable cells counted by trypan blue exclusion assay kit (Sigma, T8154) as per manufacturers protocol and data obtained were represented in bar graphs as mean±SEM.
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