Fetal cerebral cortex tissues (approximately 0.025 g) from different treatment groups were collected. Protein samples (20 μg) were separated by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The isolated protein was transferred to a polyvinylidene fluoride membrane activated with methanol, blocked with 5% skim milk, dried at room temperature for at least 1 h, and incubated with the primary antibody overnight at 4°C. Primary antibodies used for incubation were anti-TNF, anti-IL-1B, anti-NOS2, anti-NRF1, anti-HMOX1, and anti-ACTB. The sections were then incubated with secondary anti-IgG antibodies at 37°C for 90 min. Chemiluminescence (Millipore, USA) was visualized and analyzed using imaging software (GE Healthcare Life Sciences, USA). Details of antibodies against the proteins are shown in Table 3 .
Imaging software
Manufactured by Merck Group
Sourced in United States
The Imaging software is a digital tool that allows for the analysis and visualization of various types of images, including microscopic, medical, and scientific data. It provides functionalities for image processing, measurement, and quantification. The core function of the software is to enable users to capture, manipulate, and interpret visual information from different sources.
Automatically generated - may contain errors
Lab products found in correlation
Chemiluminescence, by Merck Group (2 mentions)
Anti p21 10355 1 ap, by Proteintech (1 mentions)
Anti cd11b 66519 1 ig, by Proteintech (1 mentions)
Anti β actin, by Proteintech (1 mentions)
Anti mouse igg sa00001 1, by Proteintech (1 mentions)
Anti cd86 13395 1 ap, by Proteintech (1 mentions)
Anti rabbit igg sa00001 2, by Proteintech (1 mentions)
2 protocols using imaging software
1
Protein Expression Analysis in Fetal Cerebral Cortex
2
Protein Isolation and Western Blot Analysis
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After the experiment, the brain tissues and pancreas tissues of rats were collected, and the total proteins were extracted by radio immunoprecipitation analysis (RIPA) and lysis buffer solution. The protein concentration was determined by bicinchoninic acid (BCA) method. A total of 200 μg protein samples were separated by 12% sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The isolated proteins were transferred to a polyvinylidene fluoride membrane that had been activated by methanol and blocked by 5% skim milk and dried at room temperature for at least 1 h. The primary antibodies were then incubated overnight at 4°C. The primary antibodies for incubation included anti-CD11b (66519-1-Ig, 1:2000, Proteintech, USA), anti-CD86 (13395-1-AP, 1:1000, Proteintech, USA), anti-cleaved-caspase 3 (19677-1-AP, 1:1000, Proteintech, USA), anti-p21 (10355-1-AP, 1:1000, Proteintech, USA), anti-P16 (10883-1-AP, 1:1000, Bioss, China), and anti-b-actin (60008-1-Ig, 1:5000, Proteintech, USA). They were then incubated with anti-mouse IgG (SA00001-1, 1:5000, Proteintech, USA) and antirabbit IgG (SA00001-2, 1:6000, Proteintech, USA) at 37 °C for 90 min. Chemiluminescence (Millipore, USA) was visualized and analysed using imaging software (GE Healthcare, Life Sciences, USA).
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