We referred to the database on the Microflex LT software ver. 3.1 (Bruker Daltonics) to determine the TOF peaks common to GBS. On the database, nine GBS were registered: 03 102 CTL, 03 145 CTL, 03 198 CTL, 04 158 CTL, CNR 10 CTL, 16828 DSM, 2134T DSM, 6784 DSM, and V29 CTL. In the nine GBS, several TOF peaks with intensities ≥10% were analyzed (Table S1). We then adopted four TOF peaks that were particularly specific to the nine GBS to determine the presence of GBS: Peak 1 (6,734.41 m/z; average intensities of nine GBS [6,729.59–6,736.77]), Peak 2 (6,937.95 m/z [6,932.09–6,940.77]), Peak 3 (7,963.55 m/z [7,957.35–7,966.28]), and Peak 4 (8,200.27 m/z [8,193.93–8,203.04]). Following direct MALDI detection using the Microflex LT, the TOF peaks of each tested sample was analyzed using MATLAB R2019b (MathWorks, Natick, MA, USA), a software for analyzing TOF peaks. A sample was investigated if Peak 1 to 4 were among its TOF peaks. The criteria for GBS positive on the TOF peak analysis were defined as follows: having at least one of Peak 1 to 4, an intensity of 6,000 AU or more, and a tolerance of within 4 m/z.
Microflex lt software ver 3
Manufactured by Bruker
The Microflex LT software version 3.1 is a core component of Bruker's analytical instruments. It provides the fundamental functionality to operate and manage the data acquisition process. The software enables users to control the instrument settings, acquire data, and perform basic data analysis.
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2 protocols using microflex lt software ver 3
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MALDI-TOF-Based GBS Identification
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MALDI-TOF-Based GBS Identification
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We referred to the database on the Microflex LT software ver. 3.1 (Bruker Daltonics) to determine the TOF peaks common to GBS. On the database, nine GBS were registered: 03 102 CTL, 03 145 CTL, 03 198 CTL, 04 158 CTL, CNR 10 CTL, 16828 DSM, 2134T DSM, 6784 DSM, and V29 CTL. In the nine GBS, several TOF peaks with intensities ≥10% were analyzed (Table S1). We then adopted four TOF peaks that were particularly specific to the nine GBS to determine the presence of GBS: Peak 1 (6,734.41 m/z; average intensities of nine GBS [6,729.59–6,736.77]), Peak 2 (6,937.95 m/z [6,932.09–6,940.77]), Peak 3 (7,963.55 m/z [7,957.35–7,966.28]), and Peak 4 (8,200.27 m/z [8,193.93–8,203.04]). Following direct MALDI detection using the Microflex LT, the TOF peaks of each tested sample was analyzed using MATLAB R2019b (MathWorks, Natick, MA, USA), a software for analyzing TOF peaks. A sample was investigated if Peak 1 to 4 were among its TOF peaks. The criteria for GBS positive on the TOF peak analysis were defined as follows: having at least one of Peak 1 to 4, an intensity of 6,000 AU or more, and a tolerance of within 4 m/z.
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