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Acl top 300

Manufactured by Werfen
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The ACL TOP 300 is a fully automated coagulation analyzer designed for high-volume clinical laboratories. It is capable of performing a wide range of routine and specialty coagulation tests. The ACL TOP 300 features advanced technology and provides efficient, reliable, and consistent results.

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Lab products found in correlation

Hemosil protein c, by Werfen (1 mentions) Recombiplastin 2g, by Werfen (1 mentions) Enzygnost tat micro kit, by Siemens (1 mentions) Hemosil aptt sp, by Werfen (1 mentions) Acl top 500, by Werfen (1 mentions) Cs2100i, by Sysmex (1 mentions) Au680 clinical chemistry analyzer, by Beckman Coulter (1 mentions) Dxh 800 hematology analyzer, by Beckman Coulter (1 mentions) Alinity i system, by Abbott (1 mentions) Cobas e411, by Roche (1 mentions)

10 protocols using acl top 300

1

Coagulation Marker Measurement Protocol

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The international normalized ratio (INR) was assessed with commercially available methods on an automated coagulation analyzer (ACL 300 TOP) with reagents (Recombiplastin 2G) and protocols from the manufacturer (Instrumentation Laboratory).
Levels of fibrinogen and antithrombin were assessed on an automated coagulation analyser (ACL 300 TOP). We used QFA Thrombin (Hemosil) for fibrinogen and Liquid Antithrombin for antithrombin. Testing was performed according to the protocols from the manufacturer (Instrumentation Laboratory).
Bos S., van den Boom B., Ow T., Prachalias A., Adelmeijer J., Phoolchund A., Dunsire F., Milan Z., Roest M., Heaton N., Bernal W, & Lisman T. (2020). Efficacy of pro‐ and anticoagulant strategies in plasma of patients undergoing hepatobiliary surgery. Journal of Thrombosis and Haemostasis, 18(11), 2840-2851.
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2

Coagulation Factors and Inhibitors Measurement

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Levels of factor (F) VIII, II, antithrombin (AT) and Protein C were measured on an automated coagulation analyzer (ACL 300 TOP) with reagents and protocols from the manufacturer (Recombiplastin 2G and FII depleted plasma for FII, Hemosil (R) SynthASil and FVIII depleted plasma for FVIII, Liquid Antithrombin reagent for AT, and Hemosil Protein C for Protein C measurements; Instrumentation Laboratory, Breda, the Netherlands).
Plasma levels of Tissue Factor Pathway Inhibitor (TFPI) were determined with an in-house ELISA as previously described
18 (link).
Arshad F., Adelmeijer J., Blokzijl H., van den Berg A., Porte R, & Lisman T. (2014). Abnormal hemostatic function one year after orthotopic liver transplantation can be fully attributed to endothelial cell activation. F1000Research, 3, 103.
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3

Comprehensive Blood Panel Analysis

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Haemoglobin, white blood cell count, albumin, creatinine, bilirubin, aspartate transaminase, alanine transaminase and gamma‐glutamyl transpeptidase were measured in the diagnostic laboratory of King's College Hospital for routine clinical care. International normalized ratios, and plasma levels of fibrinogen, antithrombin, factor II, factor VIII and factor X were measured in stored frozen samples on an automated coagulation analyzer (ACL 300 TOP) with reagents and protocols from the manufacturer (Werfen, Breda, The Netherlands).
Lisman T., Kleiss S., Patel V.C., Fisher C., Adelmeijer J., Bos S., Singanayagam A., Stoy S.H., Shawcross D.L, & Bernal W. (2018). In vitro efficacy of pro‐ and anticoagulant strategies in compensated and acutely ill patients with cirrhosis. Liver International, 38(11), 1988-1996.
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4

Coagulation Assays in Clinical Research

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The INR, activated partial thromboplastin time (APTT), coagulation factor II, factor X, and AT levels were assessed using the STA Compact Max3 analyzer with reagents and protocols from the manufacturer (Stago). Clauss fibrinogen was measured on an automated coagulation analyzer (ACL 300 TOP) with reagents and protocols from the manufacturer (Werfen). Levels of von Willebrand factor (VWF) were assessed with in house enzyme‐linked immunosorbent assay using commercially available polyclonal antibodies against VWF (DAKO). Levels of thrombin‐antithrombin (TAT) complexes were measured to assess in vivo thrombin generation using the Enzygnost TAT micro kit (Siemens Healthcare Diagnostics).
van den Boom B.P., von Meijenfeldt F.A., Adelmeijer J., Roberts L.N., Bernal W, & Lisman T. (2021). Heparins have adequate ex vivo anticoagulant effects in hospitalized patients with cirrhosis. Journal of Thrombosis and Haemostasis, 19(6), 1472-1482.
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5

Comprehensive Hemostatic Assessment Protocol

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Primary haemostasis was assessed by measurement of platelet count, levels of von Willebrand factor (VWF) and its metalloproteinase inhibitor, ADAMTS 13, as previously described [21] . Coagulation was assessed by measurement of traditional markers of coagulation, prothrombin time (PT), international normalized ratio (INR), activated partial thromboplastin time (APTT), and specific markers including antithrombin, fibrinogen, and factor VIII levels using a an automated coagulation analyzer (ACL 300 TOP) with reagents and protocols from the manufacturer (Werfen, Breda, The Netherlands) as described previously [22] . Thrombin generation testing was performed in absence and presence of thrombomodulin using protocols and reagents from Thrombinoscope (Maastricht, The Netherlands) as described previously [23] . Thrombin generation curves (thrombin generation vs time) allowed calculation of endogenous thrombin potential (ETP) (nM * min) as area under the curve, thrombin lag-time (min) as time to commencement of thrombin generation, thrombin velocity index (nM/min) as the rate of thrombin generation, and thrombin peak (nM) as peak value of thrombin generation. Fibrinolysis was assessed by a plasmabased clot lysis time as described previously [23] and measurement of plasminogen activation-inhibitor type 1 (PAI-1).
Fig. 1. a-b. Measures of primary haemostasis.
Fisher C., Patel V.C., Stoy S.H., Singanayagam A., Adelmeijer J., Wendon J., Shawcross D.L., Lisman T, & Bernal W. (2018). Balanced haemostasis with both hypo- and hyper-coagulable features in critically ill patients with acute-on-chronic-liver failure. Journal of critical care, 43.
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6

Coagulation Assays for Heparin Monitoring

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aPTT was determined using HemosIL aPTT-SP (silicate activator; Instrumentation Laboratory, Bedford, Massachusetts, USA). Fifty microlitres of patient plasma were mixed with 50 µl of aPTT-SP. TT is a coagulation test measuring the time to fibrin formation after adding thrombin.23 Because of a high sensitivity for heparin, two thrombin concentrations are necessary to cover the full spectrum of heparin concentration. We used 3 U/mL (TT 1) and 7.5 U/mL (TT 2) of bovine thrombin (HemosIL TT; Instrumentation Laboratory, Bedford, Massachusetts, USA). TT 2 was determined in case of an unclottable TT 1 corresponding to a high concentration of heparin. Anti-Xa activity was determined using HemosIL liquid anti-Xa (Instrumentation Laboratory, Bedford, Massachusetts, USA), calibrated with HemosIL heparin calibrators. PiCT was measured using Pefakit PiCT (DSM Pentapharm, Basel, Switzerland). The test has been described in detail elsewhere.19 24 (link) Briefly, 50 µL of patient plasma was incubated for 180 s with an activator containing activated factor Xa, phospholipids and Russell’s viper venom. Coagulation was initiated with calcium chloride and clotting time (s) measured. All analyses were performed on an ACL TOP 300 (Instrumentation Laboratory, Bedford, Massachusetts, USA).
Bürki S., Brand B., Escher R., Wuillemin W.A, & Nagler M. (2018). Accuracy, reproducibility and costs of different laboratory assays for the monitoring of unfractionated heparin in clinical practice: a prospective evaluation study and survey among Swiss institutions. BMJ Open, 8(6), e022943.
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7

Coagulation Profile in Canine Plasma

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Prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), fibrinogen concentration, and activities of coagulation factors V (FV), VII (FVII), VIII (FVIII), IX (FIX), X (FX), von Willebrand factor antigen (vWF), antithrombin (AT), and protein C (PC) were measured in citrated plasma using a commercial turbidimetric analyzer (ACL-TOP 300, Instrumentation Laboratory, Werfen, Artarmon, Australia). The coagulation times, PT, APTT, and TT were measured using the manufacturer’s reagents. Fibrinogen concentration was measured using the manufacturer’s reagent for the Clauss method, calibrated using the manufacturer’s calibration plasma. All remaining coagulation factor activities were calibrated with canine pooled plasma, as previously described [20 (link)]. Activity of FV, FVII, and FX were measured using a modified PT test, while FVIII and FIX were measured using a modified APTT test, all using the manufacturer’s specific factor-depleted plasmas. A latex-enhanced immunoassay was used to measure vWF. Automated chromogenic assays were used to measure AT and PC. Samples were thawed in batches using a temperature-controlled water bath at 37 °C. Assay order was determined by the analyzer’s high-throughput algorithm. Quality controls were run daily. Coagulation results were compared to published reference intervals where available [21 (link)].
Boyd C.J., Raisis A.L., Sharp C.R., Claus M.A., Hosgood G, & Smart L. (2022). Biomarkers of Coagulation and Inflammation in Dogs after Randomized Administration of 6% Hydroxyethyl Starch 130/0.4 or Hartmann’s Solution. Animals : an Open Access Journal from MDPI, 12(19), 2691.
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8

Routine Coagulation Assays in Hospital

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Other routinely available in the hospital setting coagulation assays APTT, PT, INR, and fibrinogen were performed with a Sysmex CS 2100i automated coagulometer (Sysmex Corporation, Kobe, Japan), ACL TOP 500 (Instrumentation Laboratory, MA, USA) and ACL TOP 300 (Instrumentation Laboratory, MA, USA).
Bulanov A.Y., Bulanova E.L., Simarova I.B., Bovt E.A., Eliseeva O.O., Shakhidzhanov S.S., Panteleev M.A., Roumiantsev A.G., Ataullakhanov F.I, & Karamzin S.S. (2023). Integral assays of hemostasis in hospitalized patients with COVID-19 on admission and during heparin thromboprophylaxis. PLOS ONE, 18(6), e0282939.
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9

Comprehensive Biochemical Profiling in Hospital Admission

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All biochemical procedures were done on the first day of hospital admission in a specialized biochemical laboratory of the Clinical Center Kragujevac, Serbia. Complete blood cell count (CBC) was measured using a hematology analyzer (DxH 800 Hematology Analyzer by Beckman Coulter). The biochemical parameters such as glucose, creatinine, urea, cholesterol, triglyceride (TG), aspartate aminotransferase (AST), alanine aminotransferase (ALT) gamma-glutamyl transferase (GGT), lactate dehydrogenase (LDH), total and direct bilirubin, C-reactive protein (CRP), sodium, and potassium were estimated from the serum samples by using standard kits in an automatic clinical chemistry analyzer (AU680 Clinical Chemistry Analyzer by Beckman Coulter). Measurement of the vitamin D level was performed using an automated immunoassay analyzer—the Alinity i system (Abbott Laboratories, IL, USA) that utilizes the chemiluminescent microparticle immunoassay (CMIA) principle. The level of procalcitonin in the serum was determined by the method of electrochemiluminescence, on the immunochemistry analyzer (Cobas e 411 by Roche). D dimer concentration measurement was performed on coagulation analyzer ACL-TOP 300 (Instrumentation Laboratory, Bedford, USA) employing the automated latex-enhanced particle immunoturbidimetric method.
Cekerevac I., Turnic T.N., Draginic N., Andjic M., Zivkovic V., Simovic S., Susa R., Novkovic L., Mijailovic Z., Andjelkovic M., Vukicevic V., Vulovic T, & Jakovljevic V. (2021). Predicting Severity and Intrahospital Mortality in COVID-19: The Place and Role of Oxidative Stress. Oxidative Medicine and Cellular Longevity, 2021, 6615787.
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10

Clotting Activity Determination by APTT

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FIX clotting activity levels were determined by one-stage activated partial thromboplastin time (APTT) assay using coagulometric (turbidimetric) principle on the ACL TOP 300 (Instrumentation Laboratory). Plasma samples were mixed with hFIX-deficient plasma and the values were compared with a reference standard consisting of serial dilutions of normal human plasma in hFIX-deficient plasma. Baseline clotting activity was determined in multiple pre-treatment samples and the clotting activity post-treatment was corrected for the endogenous baseline levels.
Spronck E.A., Liu Y.P., Lubelski J., Ehlert E., Gielen S., Montenegro-Miranda P., de Haan M., Nijmeijer B., Ferreira V., Petry H, & van Deventer S.J. (2019). Enhanced Factor IX Activity following Administration of AAV5-R338L “Padua” Factor IX versus AAV5 WT Human Factor IX in NHPs. Molecular Therapy. Methods & Clinical Development, 15, 221-231.
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