647 anti mouse

Cells were rinsed with 1 × PBS, fixed in 4% PFA, and permeabilized with 0.1 M Tris-buffered saline (TBS; pH 7.6) containing 0.5% Triton X-100 for 5 min, then blocked with 3% goat serum in TBS, incubated with the primary antibody in TBS containing 1% goat serum overnight at 4°C and then with the secondary antibody for 1 h at room temperature. Immunolabeled cells were mounted in VECTASHIELD^® antifade mounting media with or without DAPI (Vector Laboratories), then examined under a confocal microscope. Primary antibodies included mouse against GBA (Abcam) and mouse against HA (Sigma), and the secondary antibodies include the Alexa Fluor 568 and the 647 anti-mouse (Thermo Fisher Scientific).

Primary antibodies used in this study are as follows: anti-GFP/YFP (ThermoFisher Scientific, A10262) 1:1000, anti-Cytokeratin 14 (Covance, PRB-155P) 1:2000, anti-Cytokeratin 6 (Biolegend, 905701) 1:2000, anti-pericentrin (abcam, ab4448) 1:1000, anti-CD45 (Biolegend, 103102) 1:500, anti-CD31 (abcam, ab7388) 1:1000, anti-Loricrin (Covance PRB-145P) 1:2000, Alexa Fluor 647 anti-CD49f (Biolegend, 313610) 1:250.
All secondary antibodies are Alexa Fluor conjugated and diluted 1:1000 in staining buffer: 488 anti-chicken (Jackson ImmunoResearch, 703-545-155), 555 anti-rabbit (ThermoFisher Scientific, A31572), 647 anti-mouse (ThermoFisher Scientific, A32787), 488 and 647 anti-rat (ThermoFisher Scientific, A21208 and A48272).