Myc tag 71d10

Manufactured by Cell Signaling Technology

Sourced in United Kingdom

Myc-Tag-71D10 is a monoclonal antibody that specifically recognizes the Myc epitope tag. It is a useful tool for the detection and immunoprecipitation of Myc-tagged proteins in various experimental applications.

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Lab products found in correlation

α flag, by Merck Group (2 mentions) αcdc73, by Fortis Life Sciences (2 mentions) αh3k4me3, by Abcam (2 mentions) αleo1, by Fortis Life Sciences (2 mentions) Stat5 y694, by Cell Signaling Technology (2 mentions) N acetyl l cysteine, by Merck Group (1 mentions) Mouse anti flag f1804, by Merck Group (1 mentions) 2 3 cgamp, by InvivoGen (1 mentions) Anti β actin, by Santa Cruz Biotechnology (1 mentions) Sting d2p2f, by Cell Signaling Technology (1 mentions) Cgas d3o8o, by Cell Signaling Technology (1 mentions) Rig 1 d14g6, by Cell Signaling Technology (1 mentions) Dmxaa, by InvivoGen (1 mentions) H 151, by InvivoGen (1 mentions) Myc tag 9b11, by Cell Signaling Technology (1 mentions) Influx cell sorter, by BD (1 mentions) Lsrfortessa, by BD (1 mentions) Actin 20 33, by Merck Group (1 mentions) Perk sc 7383 and sc 16982 r, by Santa Cruz Biotechnology (1 mentions) Etv5 wh0002119m2, by Merck Group (1 mentions)

Close spelling variants of this product

Myc-tag (195 citations) Rabbit anti-myc tag (71D10, (3 citations) α-Myc-tag (71D10, (2 citations) Myc-Tag (71D10) Rabbit mAb (2 citations)

5 protocols using myc tag 71d10

ChIP-qPCR Protocol for Chromatin Analysis

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ChIP-qPCR was performed as described^{64 (link)}, using αCDC73, αLEO1 (Bethyl, Montgomery, TX), αMLLc (gift from Dr. Yali Dou), αFlag (Sigma), αH3K4me3, αH3 (Abcam, Cambridge, UK), IgG (Santa Cruz, Dallas, TX), Stat5-Y694 and Myc-Tag-71D10 (Cell Signaling, Danvers, MA). qPCR primer sequences are listed (Table S1).

Serio J., Ropa J., Chen W., Mysliwski M., Saha N., Chen L., Wang J., Miao H., Cierpicki T., Grembecka J, & Muntean A.G. (2017). The PAF Complex Regulation of Prmt5 Facilitates the Progression and Maintenance of MLL-Fusion Leukemia. Oncogene, 37(4), 450-460.

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ChIP-qPCR Protocol for Chromatin Analysis

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Murine Norovirus Protein Detection

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DMXAA, H151 and 2ʹ3ʹ-cGAMP were purchased from InvivoGen. JAK inhibitor 1 (SC-204021) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). N-Acetyl-L-cysteine was purchased from Sigma-Aldrich. Rabbit polyclonal antisera to MNV NS1/2 was kindly provided by Prof. Vernon K. Ward (School of Biomedical Sciences, University of Otago, New Zealand).^{47 (link)} Rabbit polyclonal antisera to MNV NS7 was kindly provided by Prof. Ian Goodfellow (Department of Pathology, University of Cambridge, UK).^{48 (link)} Antibodies against STAT1 (#9172), pSTAT1 (Ser727, #9177), RIG-I (D14G6, #3743), MDA5 (D74E4, #5321), cGAS (D3O8O, #31659), STING (D2P2F, #13647), Myc-tag (71D10, #2278) and Myc-Tag (9B11, #2276) were purchased from Cell Signaling Technology. Rabbit anti-GBP2 (11854-1-AP) and anti-GBP5 (13220-1-AP) antibodies were purchased from Proteintech. Mouse anti-Flag (F1804, Sigma-Aldrich) and anti-β-actin (#sc-47778, Santa Cruz Biotechnology) antibodies were used. Anti-rabbit and anti-mouse IRDye-conjugated secondary antibodies (Li-Cor Bioscience, Lincoln, USA) were used.

Yu P., Miao Z., Li Y., Bansal R., Peppelenbosch M.P, & Pan Q. (2021). cGAS-STING effectively restricts murine norovirus infection but antagonizes the antiviral action of N-terminus of RIG-I in mouse macrophages. Gut Microbes, 13(1), 1959839.

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Generation of UBL3 Expressing Cell Lines

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To generate cell lines expressing wild type or mutant UBL3 constructs, Myc-tagged wild type or C114S mutant Ubl3 cDNA was purchased (Biomatik) and cloned into p-MSCV-IRES-eGFP II (Addgene plasmid #52107), which was a gift from Dario Vignali. Single-cell cloned ΔUbl3 MutuDCs were transduced with retroviral supernatant, and GFP^high expressing cells were sorted with a Beckton Dickinson Influx cell sorter (BD Biosciences). Expression of Myc-tagged UBL3 constructs was confirmed by western blotting using antibodies against UBL3 (ab113820, Abcam), Myc-tag (71D10, Cell Signaling Technologies), and actin (20-33, Sigma) antibodies. For analysis of surface markers, cells were stained with antibodies against MHC II (M5/114) and CD86 (GL-1) and analyzed by flow cytometry on the LSRFortessa (BD Biosciences). For full details of antibodies, see Supplementary Tables 3, 4.

Liu H., Wilson K.R., Firth A.M., Macri C., Schriek P., Blum A.B., Villar J., Wormald S., Shambrook M., Xu B., Lim H.J., McWilliam H.E., Hill A.F., Edgington-Mitchell L.E., Caminschi I., Lahoud M.H., Segura E., Herold M.J., Villadangos J.A, & Mintern J.D. (2022). Ubiquitin-like protein 3 (UBL3) is required for MARCH ubiquitination of major histocompatibility complex class II and CD86. Nature Communications, 13, 1934.

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Antibody Source and Dilution Protocol

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The following antibodies were obtained from Cell Signaling Technologies: HA (C29F4) (1:6000), Lamin(A/C) (2032), pERK (4370), ubiquitin (3933), PJA2 (40180), and β-TrCP (D13F10), β-actin (8H10D10) (1:20,000), myc tag (71D10), and α-tubulin (2144) (1:5000). pERK (sc-7383 and sc-16982-R), PJA1 (sc-517068), and GFP (sc-9996) (1:6000) were obtained from Santa Cruz Biotechnology. Ki67 was obtained from Dako. Phospho Ser/Thr (ab17464), T7 tag (ab9138), capicua (ab123822), ETV1 (ab81086), Renilla (ab187338), and myc protein (ab3207) were obtained from Abcam. The following antibodies were purchased from Millipore T7 tag (69522), capicua (ABN446) and capicua (MABN449). FLAG-M2 (F1804), β-actin (A5316) (1:10,000), vinculin (V9264) (1:30,000), ETV5 (WH0002119M2), and polyclonal ERK (M5670) antibodies (1:5000) were obtained from Sigma. PJA1 (MBS153701) was purchased from MyBioSource.com. All antibodies were utilized at a 1:1,000 dilution unless otherwise specified.

Bunda S., Heir P., Metcalf J., Li A.S., Agnihotri S., Pusch S., Yasin M., Li M., Burrell K., Mansouri S., Singh O., Wilson M., Alamsahebpour A., Nejad R., Choi B., Kim D., von Deimling A., Zadeh G, & Aldape K. (2019). CIC protein instability contributes to tumorigenesis in glioblastoma. Nature Communications, 10, 661.

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