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Anti actin c4 from

Manufactured by MP Biomedicals
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Sourced in United States

Anti-actin (C4) is a monoclonal antibody produced by MP Biomedicals. It is designed to recognize the C-terminal domain of actin, a highly conserved cytoskeletal protein found in all eukaryotic cells.

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Lab products found in correlation

Anti geminin clone e5q9s, by Thermo Fisher Scientific (2 mentions) Anti phospho atm ser1981 clone 10h11, by Thermo Fisher Scientific (2 mentions) Anti atm clone d2e2, by Thermo Fisher Scientific (2 mentions) Polyclonal anti phospho atr ser428, by Merck Group (2 mentions) Polyclonal anti phospho atr thr1989, by Merck Group (2 mentions) Anti atr clone e1s3s, by Merck Group (2 mentions) Anti phospho chk1 ser317 clone d12h3, by Merck Group (2 mentions) Anti phospho chk2 thr68 clone c13c1, by Merck Group (2 mentions) Anti mdm2 clone smp14, by Merck Group (2 mentions) Anti p53 clone 1c12, by BD (2 mentions) Anti p21 clone sxm30 ruo, by BD (2 mentions) Anti γh2ax, by Merck Group (2 mentions) Anti caspase 2 clone 11b4, by Merck Group (2 mentions) Anti caspase 1 d7f10, by Cell Signaling Technology (2 mentions) Anti caspase 5 d3g4w, by Cell Signaling Technology (2 mentions) Anti gsdmd g7422 from, by Merck Group (2 mentions) Anti gsdmd n term e7h9g, by Cell Signaling Technology (2 mentions) Anti il 1β mab601, by R&D Systems (2 mentions) Ultrapure lps from e coli o111 b4, by InvivoGen (2 mentions)

4 protocols using anti actin c4 from

1

Immunoblotting Antibodies for DNA Damage Response

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Unless otherwise indicated antibodies were purchased from Cell Signaling Technology (Danvers, MA). The following antibodies were used: anti-caspase-2 (clone 11B4 from Millipore); anti-Geminin (Clone E5Q9S); anti-CDT1 (Clone D10F11); anti-phospho-ATM (Ser1981) (clone 10H11 from Invitrogen and clone 10H11.E12); anti-ATM (clone D2E2); polyclonal anti-phospho-ATR (Ser428); polyclonal anti-phospho-ATR (Thr1989); anti-ATR (clone E1S3S); anti-phospho-Chk1 (Ser317) (clone D12H3); anti-Chk1 (clone 2G1D5); anti-phospho-Chk2 (Thr68) (clone C13C1); anti-Chk2 (polyclonal); anti-MDM2 (clone SMP14, Sigma-Aldrich); anti-p53 (clone 1C12); anti-p21 (clone SXM30(RUO), BD Biosciences); anti-actin (C4 from MP Biomedicals); and anti-γ-H2AX (EMD Millipore). All cell culture media reagents were purchased from Thermo Fisher (Carlsbad, CA, USA). Unless otherwise indicated, all other reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Boice A.G., Lopez K.E., Pandita R.K., Parsons M.J., Charendoff C.I., Charaka V., Carisey A.F., Pandita T.K, & Bouchier-Hayes L. (2021). Caspase-2 regulates S-phase cell cycle events to protect from DNA damage accumulation independent of apoptosis. Oncogene, 41(2), 204-219.
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2

Immunoblotting Antibodies for DNA Damage Response

Check if the same lab product or an alternative is used in the 5 most similar protocols
Unless otherwise indicated antibodies were purchased from Cell Signaling Technology (Danvers, MA). The following antibodies were used: anti-caspase-2 (clone 11B4 from Millipore); anti-Geminin (Clone E5Q9S); anti-CDT1 (Clone D10F11); anti-phospho-ATM (Ser1981) (clone 10H11 from Invitrogen and clone 10H11.E12); anti-ATM (clone D2E2); polyclonal anti-phospho-ATR (Ser428); polyclonal anti-phospho-ATR (Thr1989); anti-ATR (clone E1S3S); anti-phospho-Chk1 (Ser317) (clone D12H3); anti-Chk1 (clone 2G1D5); anti-phospho-Chk2 (Thr68) (clone C13C1); anti-Chk2 (polyclonal); anti-MDM2 (clone SMP14, Sigma-Aldrich); anti-p53 (clone 1C12); anti-p21 (clone SXM30(RUO), BD Biosciences); anti-actin (C4 from MP Biomedicals); and anti-γ-H2AX (EMD Millipore). All cell culture media reagents were purchased from Thermo Fisher (Carlsbad, CA, USA). Unless otherwise indicated, all other reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Boice A.G., Lopez K.E., Pandita R.K., Parsons M.J., Charendoff C.I., Charaka V., Carisey A.F., Pandita T.K, & Bouchier-Hayes L. (2021). Caspase-2 regulates S-phase cell cycle events to protect from DNA damage accumulation independent of apoptosis. Oncogene, 41(2), 204-219.
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3

Apoptosis and Pyroptosis Pathway Analysis

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The following antibodies were used: anti-Caspase-1 (D7F10 from Cell Signaling Technology, Danvers, MA, USA), anti-Caspase-4 (4450 from Cell Signaling Technology, Danvers, MA, USA), anti-Caspase-5 (D3G4W from Cell Signaling Technology, Danvers, MA, USA); anti-actin (C4 from MP Biomedicals); anti-GSDMD (G7422 from Sigma-Aldrich, St. Louis, MO, USA), anti-GSDMD N-term (E7H9G from Cell Signaling Technology, Danvers, MA, USA), anti-IL-1β (MAB601, R&D Systems, Inc., Minneapolis, MN, USA). All cell culture media reagents were purchased from Thermo Fisher (Waltham, MA, USA). Ultrapure LPS (from E. coli O111:B4) was purchased from Invivogen (San Diego, CA, USA). Unless otherwise indicated, all other reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Bolívar B.E., Brown-Suedel A.N., Rohrman B.A., Charendoff C.I., Yazdani V., Belcher J.D., Vercellotti G.M., Flanagan J.M, & Bouchier-Hayes L. (2021). Non-canonical roles of caspase-4 and caspase-5 in heme driven- IL-1β release and cell death. Journal of immunology (Baltimore, Md. : 1950), 206(8), 1878-1889.
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4

Inflammasome Signaling Pathway Analysis

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The following antibodies were used: anti-Caspase-1 (D7F10 from Cell Signaling Technology, Danvers, MA, USA), anti-Caspase-4 (4450 from Cell Signaling Technology, Danvers, MA, USA), anti-Caspase-5 (D3G4W from Cell Signaling Technology, Danvers, MA, USA); anti-actin (C4 from MP Biomedicals); anti-GSDMD (G7422 from Sigma-Aldrich, St. Louis, MO, USA), anti-GSDMD N-term (E7H9G from Cell Signaling Technology, Danvers, MA, USA), anti-IL-1β (MAB601, R&D Systems, Inc., Minneapolis, MN, USA), anti-IL-1β cleaved, Asp116 (PA5-105048 from Thermo Fisher, Waltham, MA, USA). All cell culture media reagents were purchased from Thermo Fisher (Waltham, MA, USA). Ultrapure LPS (from E. coli O111:B4) was purchased from Invivogen (San Diego, CA, USA). Unless otherwise indicated, all other reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Bolívar B.E., Brown A.N., Rohrman B.A., Charendoff C.I., Yazdani V., Belcher J.D., Vercellotti G.M., Flanagan J.M., & Bouchier‐Hayes L. (2020). Non-canonical roles of caspase-4 and caspase-5 in heme driven- IL-1β release and cell death.
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