The following reagents were from commercial sources: goat anti-mouse LDLR, horseradish peroxidase (HRP)-conjugated anti-mouse PCSK9 antibodies (Ab), and PCSK9 enzyme-linked immunosorbent assay (ELISA) kit from R&D Systems; mouse anti-β-actin Ab, HRP-conjugated anti-goat Ab, and lipoprotein-depleted serum (LPDS) from Sigma; plasmid vectors pCIneo and pGL4.13-luc2 as well as the Luciferase Assay System from Promega; Western Lightning Chemiluminescence Reagent Plus from Perkin-Elmer; plasma lipids assay kits from BioVision; RNeasy extraction kit and Ni-NTA agarose from Qiagen; Superscript II RNase H– Reverse Transcriptase, PCR primers, HRP-conjugated anti-V5 tag Ab, and Lipofectamine Reagent Plus from Invitrogen; FastStart TaqMan ProbeMaster-Rox master mix, Universal Probe Library (UPL) fluorescent probes, and the Protease Inhibitor Cocktail (PIC) from Roche.
Goat anti mouse ldlr
Manufactured by R&D Systems
Goat anti-mouse LDLR is a laboratory reagent used for the detection and analysis of the low-density lipoprotein receptor (LDLR) protein in mouse samples. It is a polyclonal antibody produced in goats and specifically recognizes the mouse LDLR protein.
Automatically generated - may contain errors
Lab products found in correlation
Faststart taqman probemaster rox master mix, by Roche (1 mentions)
Mouse anti β actin ab, by Merck Group (1 mentions)
Luciferase assay system, by Promega (1 mentions)
Superscript 2 rnase h reverse transcriptase, by Thermo Fisher Scientific (1 mentions)
Protease inhibitor cocktail, by Roche (1 mentions)
Lipofectamine plus reagent, by Thermo Fisher Scientific (1 mentions)
Western lightning chemiluminescence reagent plus, by PerkinElmer (1 mentions)
Ripa lysis buffer system, by Santa Cruz Biotechnology (1 mentions)
Supersignal west pico chemiluminescent reagent, by Thermo Fisher Scientific (1 mentions)
Nitrocellulose membrane, by Bio-Rad (1 mentions)
2 protocols using goat anti mouse ldlr
1
Detailed PCSK9 Protein Assay Protocol
2
Quantification of Liver LDLR Protein
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Mouse or rhesus macaque liver samples (~100 mg) were homogenized using the RIPA Lysis Buffer System (Santa Cruz Biotechnology) following the manufacturer’s protocol. Liver lysates were collected following centrifugation at 10,000 x g for 30 min. Protein samples were separated by SDS-PAGE and transferred onto nitrocellulose membranes (Bio-Rad). Membranes were probed using goat anti-mouse LDLR at a 1:500 dilution (catalogue number AF2255; R&D Systems) or goat anti-human LDLR at a 1:500 dilution (catalogue number AF2148; R&D Systems) followed by incubation with an anti-goat IgG horseradish peroxidase-conjugated secondary antibody at a 1:1000 dilution (catalogue number HAF107; R&D Systems). As a control, blots were also probed with an HRP-labeled rabbit anti-ß-actin monoclonal antibody at a 1:1000 dilution (catalogue number #5152; Cell Signaling Technology). Western blots were visualized with SuperSignal West Pico Chemiluminescent reagents (Thermo Fisher Scientific) and band intensities were quantified using ImageJ software.
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