SWCNTs with high purity were purchased from Chengdu Organic Chemicals Co. Ltd., Chinese Academy of Sciences. Polyethyleneimine (PEI, Mw = 600 Da) was purchased from Aladdin Chemistry Co., Ltd. Doxorubicin hydrochloride (DOX) was purchased from Dalian Meilun Biological Technology Co., Ltd (Dalian, China). Cell culture medium RPMI 1640, fetal bovine serum (FBS), penicillin/streptomycin solution and fluorescent Hoechst 33342 were obtained from Beijing Solarbio Science & Technology Co., Ltd (Beijing, China). Trypsin–EDTA solution (0.25% trypsin with 0.02% EDTA) was supplied by Beyotime Biotechnology (Beijing, China). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) was purchased from Sigma-Aldrich (St. Louis, MO). NHS-PEG-COOH (Mw = 5 kDa) was purchased from Shanghai Ponsure Biotechnology Co., Ltd. 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC·HCl) and N-hydroxysuccinimide (NHS) were purchased from Beijing Solarbio Science & Technology Co., Ltd. N,N′-Carbonyldiimidazole (CDI) was purchased from Shanghai Macklin Biochemical Technology Co., Ltd. Dicyclohexylcarbodiimide (DCC) was purchased from Aladdin. Ultrapure water was produced with a Milli-Q water system. Dimethyl sulfoxide (DMSO) was produced from Tianjin Kemiou Chemical Reagent Co., Ltd. All other reagents were of analytical grade.
N n carbonyldiimidazole cdi
Manufactured by Maclin Biochemical Technology
Sourced in China
N,N′-Carbonyldiimidazole (CDI) is a chemical compound used as a coupling agent in organic synthesis. It is a white crystalline solid that reacts with carboxylic acids to form activated esters, which can then be used to form amide bonds with amines.
Automatically generated - may contain errors
Lab products found in correlation
Acetonitrile, by Sinopharm (2 mentions)
Trypsin edta solution, by Beyotime (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
N hydroxysuccinimide nhs, by Solarbio (1 mentions)
Dimethyl sulfoxide (dmso), by Tianjin Kemiou Chemical Reagent (1 mentions)
Doxorubicin hydrochloride dox, by Meilun (1 mentions)
Acetonitrile, by Maclin Biochemical Technology (1 mentions)
4 dimethylaminopyridine dmap, by Merck Group (1 mentions)
Ferulic acid, by Maclin Biochemical Technology (1 mentions)
Nicotinic acid, by Maclin Biochemical Technology (1 mentions)
1 3 dibromopropane, by Maclin Biochemical Technology (1 mentions)
Acetone, by Sinopharm (1 mentions)
Ethyl acetate, by Sinopharm (1 mentions)
Absolute ethanol, by Sinopharm (1 mentions)
Triethylamine, by Sinopharm (1 mentions)
Dimethyl sulfoxide (dmso), by Sinopharm (1 mentions)
5 protocols using n n carbonyldiimidazole cdi
1
Doxorubicin-loaded SWCNT Nanocarriers
2
Synthesis of F127-CDI Conjugate
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12.6 g of pluronic F127 (Lutrol, Basf, Germany) was dissolved in 15 ml of acetonitrile (Sinopharm Chemical Reagent Co., Ltd., China); 1.62 g of N, N′-Carbonyldiimidazole (CDI, Shanghai Macklin Biochemical Co., Ltd., China) was dissolved in 15 ml of acetonitrile and kept from oxidizing by N2. After being dissolved, CDI was added to the F127 solution, and the reaction proceeded for 12 h. Following the reaction, the solution was dialyzed for 3 days (MWCO: 3500 D), with deionized water being replaced every 4 h. The freeze-dried white solid F127-CDI was obtained after dialysis was finished.
3
Synthesis of MTX-Conjugated PEG-Nanohydroxyapatite
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MTX, NH2-polyethylene glycol-COOH (NH2-PEG-COOH, Mw ~ 2000), N,N’-carbonyldiimidazole (CDI), and nHA were purchased from Macklin Inc. (Shanghai, China). 1-(3-(Dimethylamino)propyl)-3-ethylcarbodiimide (EDC), dimethyl sulfoxide (DMSO), and 4-dimethylamino-pyridine (DMAP) were supplied by Sigma-Aldrich (Shanghai, China).
4
Starch-based Enzymatic Modification
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Corn starch, α-amylase (AM, 50 U mg−1), amyloglucosidase (AMG, 100 U mL−1), were purchased from Shanghai Yuanye Biotechnology Co., Ltd (Shanghai, China). Ferulic acid (99%, HPLC grade), N,N′-carbonyldiimidazole (CDI), were purchased from Macklin Biochemical Technology Co. Ltd (Shanghai, China). All the reagents used were of analytical grade unless otherwise stated.
5
Synthesis of Chitooligosaccharide Lactate
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Chitooligosaccharide lactate (deacetylation degree of 94%, molecular weight ≤ 3000 Da) was obtained from Weikang Biomedical Technology Co., Ltd. (Linyi, China). Dimethyl sulfoxide (DMSO), acetonitrile, acetone, absolute ethanol, ethyl acetate, trimethylamine (33% ethanol solution) and triethylamine were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Nicotinic acid, N,N′-carbonyl diimidazole (CDI) and 1,3-dibromopropane were obtained from Macklin Biochemical Co., Ltd. (Shanghai, China). Tripropylamine and tributylamine were purchased from Aladdin Reagent Co., Ltd. (Shanghai, China). All drugs and reagents were obtained from commercial sources and used without further processing.
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