α tubulin monoclonal antibody 66031 1 ig

The PD-L1 mouse monoclonal antibody (66248-1-Ig), E-cadherin polyclonal antibody (20874-1-AP), N-cadherin polyclonal antibody (22018-1-AP), and α-tubulin monoclonal antibody (66031-1-Ig) were obtained from Proteintech (Wuhan, Hubei, China). Vimentin rabbit monoclonal antibody (#5741), anti-mouse IgG antibody (ab190475), and anti-rabbit IgG antibody (ab205718) were purchased from Abcam (Shanghai, China).

A natural product library was provided by the School of Pharmacy, Second Military Medical University (Shanghai, China). Betulonic acid (HY-N1451), arctiin (HY-N0034), and palmatine chloride (HY-N0110) were purchased from MedChemExpress. Shizukaol F was a gift from the Second Military Medical University, Shanghai, P.R. China. Gossypol (N2135) and Ro 08–2750 (B6996) were purchased from APExBIO. The above compounds were all prepared as 10 mM stocks in DMSO and aliquots stored at −20°C and then further diluted in a phosphate-buffered solution (PBS) to the desirable concentration. The MSI2 rabbit monoclonal antibody (ab76148) was purchased from Abcam. The β-actin rabbit monoclonal antibody (8457) was obtained from Cell Signaling Technology. α-Tubulin monoclonal antibody (66031-1-Ig) were purchased from Proteintech. Peroxidase-AffiniPure Goat Anti-Rabbit IgG (H+L) (111-035-003) and anti-Mouse IgG (H+L) (115-035-003) were obtained from The Jackson Laboratory. The IgG rabbit antibody (I5006) was purchased from Sigma.