Bv2 mouse microglia cells

C. parvum oocysts of the Iowa strain were purchased from a commercial source (Bunch Grass Farm, Deary, ID). The intestinal epithelial cell line (IEC4.1) was received as a kind gift from Dr. Pingchang Yang (McMaster University, Hamilton, Canada). The murine intestinal epithelial cell line, mulNTEPI cells, was purchased from InSCREENeX Cellular Screening Technologies (Germany). The BV2 mouse microglia cells and RAW264.7 mouse macrophage cells were obtained from ATCC (Manassas, VA, USA). HCT-8 cells were purchased from ATCC (Manassas, VA). Culture media were supplied with 10% FBS (Ambion) and antibiotics (100 IU/ml of penicillin and 100 μg/ml of streptomycin). Stable IEC4.1 cells with deficient in Ifnar1 or NR_033736 were generated through transfection of cells with the CRISPR/Cas9 KO^(h) (NR_033736-CRISPR/Cas9 KO and Ifnar1-CRISPR/Cas9 KO, respectively) and the HDR plasmid (NR_033736-HDR and Ifnar1-HDR plasmid, respectively), as previously described [33 (link)].

C. parvum oocysts of the Iowa strain were purchased from a commercial source (Bunch Grass Farm, Deary, ID). The mouse intestinal epithelial cell line (IEC4.1) was received as a kind gift from Dr. Pingchang Yang (McMaster University, Hamilton, Canada). The HCT-8 cells were human intestinal epithelial cells from ATCC (Manassas, Virginia). The BV2 mouse microglia cells and RAW264.7 mouse macrophage cells were obtained from ATCC. Culture media were supplied with 10% FBS (Ambion, Austin, Texas) and antibiotics (100 IU/ml of penicillin and 100 µg/ml of streptomycin).