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Human il 1β il 1f2 immunoassay

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The Human IL-1β/IL-1F2 Immunoassay is a quantitative sandwich enzyme immunoassay designed to measure the levels of human interleukin-1 beta (IL-1β) and interleukin-1 family member 2 (IL-1F2) in cell culture supernates, serum, and plasma samples.

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Lab products found in correlation

Tristar lb941 vti microplate reader, by Berthold Technologies (1 mentions) Human tnf α immunoassay, by R&D Systems (1 mentions) Quantikine hs elisa, by R&D Systems (1 mentions) Simplestep elisa kit, by Abcam (1 mentions) Tgf β, by Abcam (1 mentions)

Close spelling variants of this product

Quantikine HS ELISA human IL-1β/IL-1F2 immunoassay Quantikine Human IL-1β/IL-1f2 Immunoassay Human IL-1β IL-1β

2 protocols using human il 1β il 1f2 immunoassay

1

Quantifying Alveolar Cytokine Release

Cited 1 time
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To quantify cytokines released in the alveolar in vitro model, ELISA assays were performed. We quantified IL8 with SimpleStep ELISA® Kit (Abcam), TGF-β (Abcam) and IL1β with human IL-1β/IL-1F2 Immunoassay (R&D Systems) was titered using a commercial enzyme-linked immunosorbent assay kit (human IL-1β/IL-1F2 Immunoassay, R&D Systems) following the manufacturer’s instructions and the results were expressed as pg ml-1. All determinations were measure in same session. For IL8 quantification in BAL of COVID-19 patients we referred to already published quantifications (10 (link)).
Pandolfi L., Bozzini S., Frangipane V., Percivalle E., De Luigi A., Violatto M.B., Lopez G., Gabanti E., Carsana L., D’Amato M., Morosini M., De Amici M., Nebuloni M., Fossali T., Colombo R., Saracino L., Codullo V., Gnecchi M., Bigini P., Baldanti F., Lilleri D, & Meloni F. (2021). Neutrophil Extracellular Traps Induce the Epithelial-Mesenchymal Transition: Implications in Post-COVID-19 Fibrosis. Frontiers in Immunology, 12, 663303.
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2

Serum Cytokine Quantification Using ELISA

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Serum L-1β and TNF-α concentrations were measured using the Quantikine HS ELISA, Human IL-1β/IL-1F2 Immunoassay and Human TNF-α Immunoassay (R&D Systems) according to the attached protocol. Referring to the attached protocol, the sensitivity range for IL-1β for 25 assays was 0.014–0.063 pg/mL and the TNF-α sensitivity range for 23 assays was 0.011–0.049 pg/mL. The optical density (450 nm) after the color reaction was measured using a TriStar LB941-vTi Microplate Reader (Berthold Technologies).
Kobayashi N., Shinagawa S., Nagata T., Tagai K., Shimada K., Ishii A., Oka N., Shigeta M, & Kondo K. (2021). Blood DNA Methylation Levels in the WNT5A Gene Promoter Region: A Potential Biomarker for Agitation in Subjects with Dementia. Journal of Alzheimer's Disease, 81(4), 1601-1611.
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