Skeletal muscle was taken out from −80 °C refrigerator. Total proteins of muscle tissue were extracted using Minute TM Total Protein Extraction Kit for Animal Cultured Cells/Tissues (Invent Biotech, Plymouth, MN, USA). The proteins were used for concentration analysis under BCA Protein Assey Kit (Beyotime, Shanghai, China) and then subjected to Western blot analysis with the following antibodies: anti-ZBED6 antibody (HPA068807, 1:500, ATLAS, Stockholm, Sweden) and anti-Gapdh antibody (HRP-60004, 1:50,000, Proteintech, Chicago, IL, USA). The blots were developed using HRP-conjugated secondary antibodies. Picture were captured by imaging system (Taon 4600, Shanghai, China) and quantified by Image J software (Fiji, National Institutes of Health, Bethesda, MD, USA).
Anti gapdh antibody hrp 60004
Manufactured by Proteintech
Sourced in Sweden
The Anti-Gapdh antibody (HRP-60004) is a horseradish peroxidase (HRP)-conjugated antibody that specifically binds to the Gapdh (glyceraldehyde-3-phosphate dehydrogenase) protein. Gapdh is a commonly used endogenous control or housekeeping gene in various molecular biology techniques.
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Lab products found in correlation
2 protocols using anti gapdh antibody hrp 60004
1
ZBED6 Protein Expression in Skeletal Muscle
2
Nox2 Protein Expression Analysis
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Cells were lysed and tissues were homogenized in a Polytron homogenizer immediately after dissection in Western extraction buffer (150 mmol/L NaCl, 10% glycerol, 1% NP-40, 1 mmol/L EDTA, 20 mmol/L NaF, 30 mmol/L sodium pyrophosphate, 0.5% sodium deoxycholate, 0.05% SDS, 25 mmol/L Tris-HCl [pH 7.4]) containing a protease inhibitor cocktail (Roche). The lysate was sonicated and incubated on ice for 30 min before centrifugation. SDS-PAGE and Western blotting were performed and detected with enhanced chemiluminescence (32106; Thermo Fisher Scientific). The antibodies were anti-Nox2 antibody (SAB4300724; Sigma-Aldrich) and anti-GAPDH antibody (HRP-60004; Proteintech Group, Inc.).
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