Anti cd169

Manufactured by Bio-Rad

Sourced in United Kingdom, United States

Anti-CD169 is a lab equipment product that detects the CD169 protein. CD169 is a sialic acid-binding immunoglobulin-type lectin expressed on specific cell types. This product can be used to identify and analyze cells expressing CD169 in various research applications.

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Lab products found in correlation

Anti cd163, by Bio-Rad (1 mentions) Bs 0296g fitc, by Bioss Antibodies (1 mentions) Anti cd151, by Santa Cruz Biotechnology (1 mentions) Anti f4 80, by Thermo Fisher Scientific (1 mentions) Anti pd l1, by Thermo Fisher Scientific (1 mentions) Anti cd45r b220, by Thermo Fisher Scientific (1 mentions)

2 protocols using anti cd169

Porcine PMEC PRRSV Infection and Receptor Detection

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Porcine PMECs (4th–5th passage) were seeded into 24-well plates for immunofluorescence. To detect viral antigens, cells were incubated for 12 hr and then
infected with the PRRSV strain HN. Immunofluorescence staining for PRRSV GP5 and N proteins was carried out at 48 hpi using polyclonal antibodies (Cat #bs-4504R
and Cat #23941R, Bioss, Beijing, China). To detect the receptors of PRRSV, the PMECs were fixed with 4% paraformaldehyde after a 24-hr incubation, and a routine
indirect immunofluorescence method was performed. Anti-CD151 (Cat #sc-18753-R, Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-CD163 (Cat #MCA2311GA, AbD
Serotec, Kidlington, Oxford, UK), and anti-CD169 (Cat #MCA2316GA, AbD Serotec) antibodies and goat anti-mouse (Cat #bs-0296G-FITC, Bioss) and goat anti-rabbit
(Cat #bs-0296G-FITC, Bioss) FITC-conjugated secondary antibodies were used.

LI P., YANG Z., MA S., HU G., DONG H, & ZHANG T. (2020). Susceptibility of porcine pulmonary microvascular endothelial cells to porcine reproductive and respiratory syndrome virus. The Journal of Veterinary Medical Science, 82(9), 1404-1409.

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Immunohistochemical Profiling of Tissues

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Histologic analyses used snap-frozen tissue. Sections were stained with anti-PD-L1 (eBioscience, CA, USA), anti-CD169 (AbD Serotec, Oxfordshire, UK), anti-CD45R (B220) (eBioscience, CA, USA), anti-F4/80 (eBioscience, CA, USA) or anti-LCMV-NP (made in-house).

Shaabani N., Duhan V., Khairnar V., Gassa A., Ferrer-Tur R., Häussinger D., Recher M., Zelinskyy G., Liu J., Dittmer U., Trilling M., Scheu S., Hardt C., Lang P.A., Honke N, & Lang K.S. (2016). CD169+ macrophages regulate PD-L1 expression via type I interferon and thereby prevent severe immunopathology after LCMV infection. Cell Death & Disease, 7(11), e2446-.

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