At the end of intervention, the rat tail fasting blood samples were collected into test tubes and then centrifuged at 3000 × g for 10 minutes, and the supernatants were stored and used for the later hormonal analyses. The supernatant was used to measure for rat insulin (intra-assay CV% = 3.7%, Mercodia Ultra-sensitive Rat Insulin ELISA, Mercodia AB, Uppsal, Sweden), rat leptin (intra-assay CV% = 1.9%, BioVendor, Czech Republic), rat adiponectin (intra-assay CV% = 4.4%, AssayMaxTM, Assaypro LLC., St. Charles, MO, USA), rat corticosterone (intra-assay CV% = 7.4%, Immuno-Biological Laboratories Inc., IBL-America, Minneapolis, MN, USA), and testosterone (intra-assay CV% = 8.8%, Cayman Chemicals, Ann Arbor, MI, USA) by commercial available enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturers' instructions. At the completion of each assay, the optical density and hormonal concentration of the samples were measured and calculated using an ELISA reader detection system (Tecan GENios Microplate Reader, Switzerland).
Rat leptin
Manufactured by BioVendor
Sourced in Sweden
Rat leptin is a polypeptide hormone produced by adipose tissue in rats. It plays a key role in regulating energy balance, food intake, and body weight.
Automatically generated - may contain errors
2 protocols using rat leptin
1
Profiling Hormones in Rat Fasting
2
Lipid and Biomarker Profiling in Rats
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The levels of sera total cholesterol, LDL-C, HDL-C, and triglyceride were measured using a COBAS-C501 chemistry analyzer (Roche Diagnostics GmbH, D-68298 Mannheim, Germany). Rat homocysteine levels by the quantitative sandwich enzyme immunoassay technique (Rat homocysteine Cusabio ELISA Kit), PON-1 activities by a novel automated photometric method developed by Erel (Aslan et al., 2007) (link), adiponectin levels by the quantitative sandwich enzyme immunoassay technique (Rat adiponectin, ADP ELISA Kit), plasma leptin levels by the sandwich enzyme-linked immunosorbent method (Rat Leptin BioVendor), resistin levels by ELISA (Rat Resistin BioVendor) and FABP4 levels by ELISA technique (Rat fatty acid binding protein, BioVendor ELISA kit) were measured.
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