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Vanillin

Manufactured by J&K Scientific
Sourced in China

Vanillin is a white crystalline solid that is the primary component of the extract of the vanilla bean. It is commonly used as a food flavoring and in the production of fragrances.

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2 protocols using vanillin

1

Isolation and Characterization of Cancer Stem Cells

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Lecithin and Coumarin 6 were purchased from Sigma-Aldrich (USA). RhApoA-I and rhApoE were prepared and preserved in our laboratory. Salinomycin was purchased from Selleck (China). Vanillin was purchased from J&K scientific (China). Basic fibroblast growth factor (b-FGF) and epidermal growth factor (EGF) were bought from Peprotech (USA). B27 Supplement, DMEM medium and DMEM/F12 medium were purchased from Gibco (USA). Antihuman CD44 microbeads antibody, antihuman CD24-biotin antibody, anti-biotin microbeads, PE-conjugated antihuman CD44 antibody and FITC-conjugated antihuman CD24 antibody were purchased from Miltenyi Biotec (Germany). Antibodies against C-myc and Sox-2 were purchased from Cell Signaling Technology (USA). Antibody against LRP1 was purchased from Abcam (USA). Horseradish Peroxidase-conjugated secondary antibody, Cell Counting Kit-8 and Cell Cycle Analysis Kit were purchased from Beyotime Institute of Biotechnology (China). Annexin V-FITC/PI apoptosis detection kit was purchased from BD company (USA).
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2

Quinoa Saponins: Extraction and Characterization

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Chenopodium quinoa Willd was purchased from Jingle Yilong quinoa Co., Ltd. Esculentoside A with >98% purity was purchased from the Shanghai Yuanye Biological Technology Co., Ltd. Vanillin, glacial acetic acid, perchloric acid, and dimethyl sulfoxide (DMSO) were purchased from J & K. Acetonitrile and methanol were obtained from Fisher (American). DMEM, RPMI 1640 medium, and Penicillin‐Streptomycin Solution (100×) were purchased from Fisher Scientific (China) Co., Ltd. Fetal bovine serum (FBS) was obtained from GE Healthcare Bio‐Sciences. Annexin V‐FITC/PI Apoptosis Detection Kit was purchased from Thermo Fisher Scientific (China) Co., Ltd. Cell counting kit‐8 was purchased from Dojindo (China) CO., Ltd. The other chemicals were of analytical or HPLC grade. Quinoa saponins Qb–Qf were prepared by our laboratory determined by LC/MS and NMR, purity >98%.
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