Dimethyl sulfoxide dmso 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide

(S)-(+)-CPT (C9911), Evodiamine (E3531), sodium bicarbonate, Dimethyl sulfoxide (DMSO), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), propium iodide (PI), and Hochest 33342 were purchased from Sigma-Aldrich (St. Louis, MO, USA), Dulbecco’s modified Eagle’s medium (DMEM), trypsin, fetal bovine serum (FBS), L-glutamine, and sodium pyruvate were purchased from Gibco-BRL (Grand Island, NY, USA). An antibody against cyclin D1 was purchased from Ventana (Tucson, AZ, USA). γ-H2A.X, AMPK, phospho-AMPK, acetyl-CoA carboxylase (ACC), phospho-ACC, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were purchased from Cell Signaling (Danvers, MA, USA). Ki-67 rabbit monoclonal primary antibody was purchased form DAKO (MIB-5, Denmark). Comet Assay kit was obtained from Trevigen (Gaithersburg, MD, USA). General Layer Medium (GLM) capacity chip was obtained from Bio-Rad (Hercules, CA, USA). The lentiviral vectors (LVs), luciferase (Luc) and green fluorescent protein (GFP) vectors, and beetle luciferin were obtained from Promega (Madison, WI, USA). iView Universal DAB Detection kit was obtained from Ventana (Tucson, AZ, USA). In Situ Cell Death Detection kit, POD, was obtained from Roche Applied Science (Penzberg, Germany).

Dulbecco’s Modified Eagle’s Medium (DMEM), Roswell Park Memorial Institute (RPMI) 1640 medium, l-glutamine, fetal bovine serum (FBS), antibiotic–antimycotic, trypsin–EDTA, and phosphate-buffered saline (PBS) for cell culture were purchased from Gibco (Grand Island, NY, USA). Dimethyl sulfoxide (DMSO), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT), Hoechst 33342, propidium iodide (PI), monodansylcadaverine, rapamycin, chloroquine, and bovine serum albumin (BSA) were purchased from Sigma-Aldrich, Co. (St. Louis, MO, USA). RIPA buffer, primary antibodies (Mcl-1 (#94,296), Bcl-2 (#4223), Bax (#5023), PARP (#9532), LC3B (#2775), PI3K (#4292), p-PI3K (#4228), Akt (#9272), p-Akt (#4060), mTOR (#2983), p-mTOR (#5536), c-Myc (#18,583), and β-actin (#4970)), and secondary antibody (anti-rabbit IgG, HRP-linked (#7074)) were purchased from Cell Signaling Technology (Danvers, MA, USA). In addition, Annexin V-FITC/PI apoptosis kit was obtained from ImmunoTools (Gladiolenweg 2, Friesoythe, Germany).

STA was obtained from Cayman Chemical (Ann Arbor, MI, USA). Dimethyl sulfoxide (DMSO), 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT), acetylcholine (ACh), indomethacin, NG-nitro-L-arginine methyl ester (L-NAME), 1H- (Yin et al., 2010 (link); Kuchta et al., 2013 (link); Hu et al., 2015 (link)) Oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), 1400 W, Hcy, angiotensin II (Ang II), palmitic acid (PA) and rabbit polyclonal antibody to GTPCH1 were purchased from Sigma-Aldrich (St. Louis, MO, USA). The antibody against eNOS was purchased from Cell Signaling Technology (Danvers, MA, USA). Mouse monoclonal antibodies to DHFR and β-actin, HRP-conjugated anti-rabbit and anti-mouse IgG polyclonal antibodies were procured from Santa Cruz Biotechnology (Santa Cruz, CA, USA). ELISA kit for cGMP was obtained from R&D Systems Inc. (R&D Systems, MN, USA). BH4 ELISA kit was from MyBioSource Inc. (San Diego, CA, USA). Dulbecco’s modified Eagle medium (DMEM), fetal bovine serum (FBS) and 4-amino-5-methylamino-2′, 7′-difluorofluorescein (DAF-FM) diacetate were obtained from Invitrogen (Carlsbad, CA, USA).