Cells (5×103 cells per well) were seeded onto 96-well plates and exposed to different concentrations of resveratrol. Equal volume of Caspase-Glo 3/7 reagent was subsequently added into each well and incubated for 30 min at room temperature in the dark. The luminescence was determined using a luminometer (Berthold Sirius L; Titertek-Berthold, Pforzheim, Germany).
Berthold sirius l
Manufactured by Berthold Technologies
Sourced in Germany
The Berthold Sirius L is a luminometer designed for sensitive and precise detection of light-emitting samples. It features a high-performance photomultiplier tube detector and advanced optical design to enable accurate measurement of luminescent signals.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using berthold sirius l
1
Caspase-3/7 Activity Assay
2
Apoptosis Assay in Prostate Cancer Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PC-3 cells, LNCaP cells (4×103 cells/well) and 22RV1 cells (1×104 cells/well) were seeded in 96-well plates and exposed to MET and PTX. Equal volumes (100 µl) RPMI-1640 medium and caspase-Glo 3/7 reagent (Promega Corporation, Madison, WI, USA) were added to each well, and the cells were incubated for 30 min at room temperature in the dark. Luminescence was measured by a luminometer (Berthold Sirius L; Titertek-Berthold, Pforzheim, Germany).
3
Cell Cytotoxicity and Apoptosis Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ACHN cells(4 × 10 3 cells/well) and A498 cells (4 × 10 3 cells/well) were seeded in 96-well plates and treated with different BBR concentrations (0, 20, 50, 100 µM) for 24 h. After centrifuging at 400 × g for 5 min, the supernatants (120 μL/well) were transferred into new 96-well plates, and 60 μL of lactate dehydrogenase LDH detection reagent was added to each well. The plates were incubated for 30 min at room temperature in the dark, and the absorbance of the formazan was detected at 490 nm using a reader (EnSpire 2300 Multilabel Reader, PE, USA).
2.5 Caspase-Glo 3/7 assays ACHN cells(4 × 10 3 cells/well) and A498 cells (4 × 10 4 cells/well) were seeded in 96-well plates and treated with BBR(0, 20, 50, 100 µM) and N-Acetyl-L-cysteine (NAC) (100 µM) for 24 h. Equal volumes (100 µL) medium and caspase-Glo 3/7 reagent (Promega Corporation, Madison, WI, USA) were added to each well, and the cells were incubated at room temperature in the dark for 30 min. Luminescence was measured by a luminometer (Berthold Sirius L; Titertek-Berthold, Pforzheim, Germany).
2.5 Caspase-Glo 3/7 assays ACHN cells(4 × 10 3 cells/well) and A498 cells (4 × 10 4 cells/well) were seeded in 96-well plates and treated with BBR(0, 20, 50, 100 µM) and N-Acetyl-L-cysteine (NAC) (100 µM) for 24 h. Equal volumes (100 µL) medium and caspase-Glo 3/7 reagent (Promega Corporation, Madison, WI, USA) were added to each well, and the cells were incubated at room temperature in the dark for 30 min. Luminescence was measured by a luminometer (Berthold Sirius L; Titertek-Berthold, Pforzheim, Germany).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!