After serum-free protein blocking (Dako Cytomation), cells were hybridized with various primary antibodies (overnight at 4°C), including goat polyclonal OPN (R&D systems), mouse anti-human residues 1–16, mAb clone 6E10 (1:100; Covance)], rabbit anti-Iba1 pAb (1:250; Wako Chemicals), rat anti-CD68 mAb (1:100; Abcam), rabbit anti-iNOS pAb (1:100; Cell signaling), goat anti-MMP9 pAb (1:100; R&D systems), Rat anti-CD36 mAb (1:xx; Abcam), rabbit anti-LC3 pAb (1:250; Novus), mouse anti-Golgi mAb (1:50; Sigma-Aldrich), rabbit anti-EEA1 pAb (1:100; Millipore), rabbit anti-Rab7 mAb (1:100; Abcam), rat anti-SCARA1 mAb (1:100; AbD Serotec), and anti IL-10 pAb (1:100; R&D system). Hybridization with primary antibodies was followed by incubation with appropriate secondary polyclonal antibodies (1h at 37°C; donkey anti-mouse, anti-rat, anti-goat, and anti-rabbit; 1:200; Jackson Immuno Research Laboratories) conjugated with Cy2, Cy3, and Cy5. Sections were mounted using ProLong Gold with DAPI (Molecular Probes, Life Technologies) and analyzed as previously described.
Rabbit anti eea1 pab
Manufactured by Merck Group
Rabbit anti-EEA1 pAb is a primary antibody product that recognizes the early endosome antigen 1 (EEA1) protein. EEA1 is a marker for early endosomes and is involved in the regulation of endosome fusion. This antibody can be used in various applications, such as Western blotting, immunofluorescence, and immunohistochemistry, to detect and study the localization of EEA1 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using rabbit anti eea1 pab
1
Multimodal Immunofluorescence Profiling of Cells
2
Immunofluorescence Profiling of Alzheimer's Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After serum-free protein block (Dako Cytomation) for 1 h at room temperature, cells were incubated with the following primary antibodies diluted in blocking solution for 1 h at 37°C: mouse anti-human amyloid-beta mAb clone 6E10 (1:100; Covance), rat anti-CD36 mAb (1:200; Abcam), rat anti-CD68 mAb (1:100; Abcam), rat anti-CD204 scavenger receptor type I/II (SCARA1) mAb (1:100; AbD Serotec), rabbit anti-EEA1 pAb (1:100; Millipore), rat anti-F4/80 mAb (1:100; Abcam), goat anti-MMP-9 pAb (1:100; R&D Systems), goat anti-TREM2 mAb (1:100; Abcam), and rabbit anti-IL-34 pAb (1:100; Biorbyt). Hybridization with primary antibodies was followed by incubation with appropriate secondary polyclonal antibodies (Cy2, Cy3, or Cy5 conjugated; 1:200; Jackson ImmunoResearch Laboratories) for 30 min at 37°C. The cells were then washed 3 times in PBS before being mounted using ProLong® Gold with DAPI (Molecular Probes, Life Technologies).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!