Hsd athymic nude fox1nu mice

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The Hsd:Athymic Nude-Fox1nu mice are an immunodeficient mouse model that lacks a functional thymus gland, resulting in a lack of mature T cells. This model is commonly used in research applications that require the study of human tumor xenografts or the evaluation of potential therapies.

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Athymic nude mice Nude mice Fox1nu

2 protocols using hsd athymic nude fox1nu mice

Nude Mouse Model for Research

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All animal experiments were approved by the Animal Experimentation Committee at the Academic Medical Center in Amsterdam (AVD118002016493) and conducted in accordance with the national guidelines. Female nude (Hsd:Athymic Nude-Fox1^nu) mice (6–12 weeks old) were purchased from Envigo. The mice were housed on a 12 h light-dark cycle at 20–26 °C with 30–70% humidity. Animals were randomly assigned to experimental groups, no blinding was performed during these experiments.

Lenos K.J., Bach S., Ferreira Moreno L., ten Hoorn S., Sluiter N.R., Bootsma S., Vieira Braga F.A., Nijman L.E., van den Bosch T., Miedema D.M., van Dijk E., Ylstra B., Kulicke R., Davis F.P., Stransky N., Smolen G.A., Coebergh van den Braak R.R., IJzermans J.N., Martens J.W., Hallam S., Beggs A.D., Kops G.J., Lansu N., Bastiaenen V.P., Klaver C.E., Lecca M.C., El Makrini K., Elbers C.C., Dings M.P., van Noesel C.J., Kranenburg O., Medema J.P., Koster J., Koens L., Punt C.J., Tanis P.J., de Hingh I.H., Bijlsma M.F., Tuynman J.B, & Vermeulen L. (2022). Molecular characterization of colorectal cancer related peritoneal metastatic disease. Nature Communications, 13, 4443.

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In Vivo Anesthesia Mouse Experiment

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For in vivo experiments, we used Hsd:Athymic Nude-Fox1^NU mice (Envigo), aged 10 to 11 months. All experimental procedures were carried out in conformity with laboratory animal protocols approved by the Institutional Animal Care and Use Committee at the California Institute of Technology. The mouse was continuously supplied with 1.5% vaporized isoflurane in air to maintain stable anesthesia throughout the experiment. The anesthetic state of the mouse was confirmed by pinching its hind paw. The anesthetized mouse was laid on a customized flat animal platform that also provided anesthetic gas, and the mouse ear was sandwiched between two glass slides to act as the scattering medium. The body temperature of the mouse was maintained at 37 °C during the experiments.

Cheng Z., Li C., Khadria A., Zhang Y, & Wang L.V. (2023). High-gain and high-speed wavefront shaping through scattering media. Nature photonics, 17(4), 299-305.

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