Tauro chenodeoxycholic acid t cdca

Manufactured by Merck Group

Tauro-chenodeoxycholic acid (t-CDCA) is a bile acid derivative. It is a component used in laboratory research and analysis.

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Chenodeoxycholic acid (74 citations) Chenodeoxycholic acid (CDCA) (26 citations)

4 protocols using tauro chenodeoxycholic acid t cdca

Bile Acid and ATP Compounds Acquisition

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Sodium salt of cholic (CA) and tauro-cholic acid (t-CA), deoxycholic (DCA) and tauro-deoxycholic acid (t-DCA), chenodeoxycholic (CDCA) and tauro-chenodeoxycholic acid (t-CDCA), as well as ATP dipotassium salt were purchased from Sigma-Aldrich.

Ilyaskin A.V., Sure F., Nesterov V., Haerteis S, & Korbmacher C. (2019). Bile acids inhibit human purinergic receptor P2X4 in a heterologous expression system. The Journal of General Physiology, 151(6), 820-833.

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Synthetic Bile Acids Protocol

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Synthetic bile acids: cholic acid (CA), glyco-cholic acid (gCA), tauro-cholic acid (tCA), deoxycholic acid (DCA), glyco-deoxycholic acid (gDCA), tauro-deoxycholic acid (tDCA), chenodeoxycholic acid (CDCA), glyco-chenodeoxycholic acid (gCDCA), or tauro-chenodeoxycholic acid (tCDCA) (all from Sigma-Aldrich) were reconstituted in PBS, aliquoted, and stored at 4°C.

Cao W., Kayama H., Chen M.L., Delmas A., Sun A., Kim S.Y., Rangarajan E.S., McKevitt K., Beck A.P., Jackson C.B., Crynen G., Oikonomopoulos A., Lacey P.N., Martinez G.J., Izard T., Lorenz R.G., Rodriguez-Palacios A., Cominelli F., Abreu M.T., Hommes D.W., Koralov S.B., Takeda K, & Sundrud M.S. (2017). The xenobiotic transporter Mdr1 enforces T cell homeostasis in the presence of intestinal bile acids. Immunity, 47(6), 1182-1196.e10.

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Quantification of Bile Acids and Metabolites

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Arachidonic acid, eicosapentaenoic acid, myristic acid, 4-hydroxy phenyllactic acid, indolelactic acid, phenyllactic acid, 6′-sialyllactose, chenodeoxycholic acid (CDCA), cholic acid (CA), ursodeoxycholic acid (UDCA), lithocholic acid (LCA), glychochenodeoxycholic acid (GCDCA) and taurochenodeoxycholic acid (TCDCA) were purchased from Sigma-Aldrich (St. Louis, United States). 7-oxoLCA, 3-dehydroCDCA, 7,12-dioxoLCA, 7-oxodeoxycholic acid (7-oxoDCA), 3-dehydroCA, 7-epiCA, glycocholic acid (GCA) and taurocholic acid (TCA) were purchased from Steraloids (Newport, RI, United States). Cholic acid 7-sulfate (CA-S) and 3′-sialyllactose were purchased from Cayman (Biomol GmbH, Hamburg, Germany). Sulfate (S) conjugates of CDCA, UDCA, and LCA were synthesized according to Donazzolo et al. (Donazzolo et al., 2017 (link)) and structures were verified by MS/MS and NMR spectroscopy.
Milli-Q water (18.2 MΩ) was derived from a Milli-Q Integral Water Purification System (Billerica, MA, United States). Acetonitrile (ACN; LiChrosolv®, hypergrade for LC-MS), methanol (LiChrosolv®, hypergrade for LC-MS) and ammonium acetate (NH₄Ac) were obtained from Merck (Darmstadt, Germany). Glacial acetic acid was purchased from Biosolve (Valkenswaard, Netherlands) and formic acid from Honeywell Fluka™ (Morristown, NJ, United States).

Sillner N., Walker A., Lucio M., Maier T.V., Bazanella M., Rychlik M., Haller D, & Schmitt-Kopplin P. (2021). Longitudinal Profiles of Dietary and Microbial Metabolites in Formula- and Breastfed Infants. Frontiers in Molecular Biosciences, 8, 660456.

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Bile Acid Supplementation Protocol

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Wy was purchased from Chemsyn Science Laboratories (Lenexa, KS) and mixed into a custom grain-based diet by Research Diets (New Brunswick, NJ). CA, deoxycholic acid (DCA), CDCA, taurocholic acid (TCA), taurodeoxycholic acid (TDCA), taurochenodeoxycholic acid (TCDCA), tauroursodeoxycholic acid (TUDCA), and taurohyodeoxycholic acid (THDCA) were purchased from Sigma-Aldrich (St. Louis, MO). βMCA, ω-MCA, tauro-α-muricholic acid (TαMCA), tauro-β-muricholic acid (TβMCA), and d5-TCA were obtained from Toronto Research Chemicals (North York, ON, Canada). All other reagents were of the highest grade commercially available.

Xie C., Takahashi S., Brocker C.N., He S., Chen L., Xie G., Jang K., Gao X., Krausz K.W., Qu A., Levi M, & Gonzalez F.J. (2019). Hepatocyte peroxisome proliferator-activated receptor α regulates bile acid synthesis and transport. Biochimica et biophysica acta. Molecular and cell biology of lipids, 1864(10), 1396-1411.

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