Spectroscopic grade organic solvents (tetrahydrofuran (THF), toluene, and methanol (MeOH)) were purchased from Kanto Chemical Co., INC, Tokyo, Japan. Ultrapure water (which was purified to reach a minimum resistivity of 18.0 MΩ·cm (25 °C) using a μPure HIQ water purification system, Romax, Hanam, Korea) was utilized in all experiments.
Methanol meoh
Manufactured by Kanto Chemical
Sourced in Japan
Methanol (MeOH) is a colorless, volatile, and flammable chemical compound. It is a simple alcohol with the chemical formula CH3OH. Methanol is widely used as a solvent, a fuel, and a feedstock in the production of other chemicals.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Nacalai Tesque (2 mentions)
Dimethyl sulfoxide (dmso), by Fujifilm (2 mentions)
Acetonitrile, by Kanto Chemical (2 mentions)
Chloroform, by Kanto Chemical (1 mentions)
Dichloromethane, by Kanto Chemical (1 mentions)
Toluene, by Nacalai Tesque (1 mentions)
Hexane, by Kanto Chemical (1 mentions)
Acetonitrile, by Nacalai Tesque (1 mentions)
Acetone, by Kanto Chemical (1 mentions)
Spectroscopic grade chloroform, by Dojindo Laboratories (1 mentions)
Midazolam, by Fujifilm (1 mentions)
Medetomidine hydrochloride, by Fujifilm (1 mentions)
Penicillin, by Fujifilm (1 mentions)
Streptomycin, by Fujifilm (1 mentions)
Sodium hydroxide (naoh), by Fujifilm (1 mentions)
Dulbecco s modified eagle s medium, by Fujifilm (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Balb 3t3 clone a31, by American Type Culture Collection (1 mentions)
Balb c mice, by Sankyo Labo Service (1 mentions)
Serotonin, by Merck Group (1 mentions)
7 protocols using methanol meoh
1
Organic Solvents and Ultrapure Water
2
Metabolic Profiling of Sake Rice and Koji
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Yamadanishiki-type (polishing rate 70%) steamed rice and rice koji samples were acquired from the mainline production in Hakutsuru Sake Brewing Co., Ltd. The rice koji were collected 42 h after fermentation. Standards (sugars, sugar alcohols and amino acids) were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan), Merck & Co., Inc. (Rockville, MD, USA), Nacalai Tesque, Inc. (Kyoto, Japan), or Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). For the preparation of solvents, methanol (MeOH) was purchased from Kanto Chemical Co., Inc. (Tokyo, Japan), while ultrapure water was obtained through Barnstead GenPure xCAD Plus water purification system by Thermo Fisher Scientific (Tokyo, Japan). The matrix N-(1-naphthyl)ethylenediamine dihydrochloride (NEDC), 2,5-dihydroxybenzoic acid (DHB), and derivatization agent for amino acid imaging 2,4-diphenyl-pyranylium tetrafluoroborate (DPP-TFB) were purchased from Merck. Triethylamine (TEA) for part of the DPP-TFB buffer was obtained from Wako.
3
Synthesis of 4EPX-DDSQ Epoxy Compounds
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DDSQ containing four epoxy groups (4EPX-DDSQ) were kindly donated by JNC Corp. Toluene, dimethyl sulfoxide (DMSO) and acetonitrile were purchased respectively from Nacalai Tesque Inc. They were used without further purification. Platinum divinyl-tetramethyldisiloxane (Pt(dvs), 3 wt% in xylene solution) was obtained from Umicore. Dichloromethane, chloroform, hexane, acetone, methanol (MeOH), and ethyl ether were purchased from Kanto Chemical Co. Inc. and distilled before use. Spectroscopic grade chloroform (Dojindo Laboratories) was used as a casting solvent. Anhydrous tetrahydrofuran (THF), RuCl3·3H2O, 4′-chloro-2,2′:6′,2′′-terpyridine and 2,2′:6′,2′′-terpyridine compounds were purchased from Aldrich Chemical Co. Inc. and used as received.
4
Hydrogel Synthesis and Cell Culture
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PRX-VBn consisting of α-CDs and PEG (Mn = 5000) capped with 4-vinylbenzyl groups was synthesized as previously described by Arisaka et al. [13 (link)] (Figures S2 and S3 in Supporting Information ). Iodomethane (MeI) was purchased from Tokyo Chemical Industry (Tokyo, Japan). Methanol (MeOH) was purchased from Kanto Chemical (Tokyo, Japan). Collagen sponges for 35 mm culture dishes were purchased from Koken (Tokyo, Japan). Fetal bovine serum (FBS) was purchased from Gibco BRL, Life Technologies (Carlsbad, CA, USA). Ammonium persulfate (APS), butorphanol tartrate, dimethyl sulfoxide (DMSO), Dulbecco’s modified Eagle’s medium (high glucose, with L-glutamine, phenol red, and sodium pyruvate) (D-MEM), 100 U/mL penicillin, 100 µg/mL streptomycin, medetomidine hydrochloride, midazolam, phosphate buffered saline (PBS), sodium hydroxide (NaOH) and N,N,N’,N’-tetramethylene diamine (TEMED) were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). The BALB/3T3 clone A31, a fibroblast cell line established from mouse embryos, was obtained from the American Type Culture Collection (Manassas, VA, USA). BALB/c mice were obtained from Sankyo Labo Service (Tokyo, Japan).
5
Analytical Quantification of Neurotransmitters
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Imidacloprid (IMI) was purchased from Kanto Chemical Co., Inc. (Tokyo, Japan). DA, 3-methoxytyramine HCl (3-MT), 3-methoxytyramine-d4 HCl, and serotonin were purchased from Sigma-Aldrich (St. Louis, MO, USA). serotonin-d4 hydrochloride and histamine were purchased from Toronto Research Chemicals, Inc. (Toronto, ON, Canada), and dopamine-d4 and histamine-d4 were purchased from WATARAI Co., Ltd. (Kanagawa, Japan). DPP, which was originally purchased from Sigma Aldrich (St. Louis, MO, USA), was donated by Associate Professor Shuuichi Shimma, Department of Biotechnology, Graduate School of Engineering, Osaka University. Corn oil and formic acid were purchased from Wako Pure Chemicals Co., Inc. (Tokyo, Japan); dimethyl sulfoxide (DMSO) was purchased from Nacalai Tesque, Inc. (Kyoto, Japan); triethylamine (TEA) was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan); and methanol (MeOH) was purchased from Kanto Chemical Co., Inc.
6
Plasma and Urine Collection from Mice
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Methanol (MeOH) and acetonitrile for LC-MS were purchased from Kanto Chemical (Tokyo, Japan). Formic acid for LC-MS was purchased from Wako Pure Chemical Industries (Osaka, Japan administration, and a blood sample (2 µL) was collected in a 1.5-mL sample tube from the tail of each mouse. Phosphate-buffered saline (18 µL, including 50 nmol L -1 sodium EDTA) was immediately added, and the sample was centrifuged at 1,500 × g for 5 minutes (min) at 25°C for collecting plasma. Urine was also collected directly from each mouse with a 1.5-mL sample tube.
7
Antioxidant Compound Protocol Analysis
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FMT, RF, dimethyl sulfoxide (DMSO), imidazole, nitroblue tetrazolium (NBT), p-nitrosodimethylaniline (RNO), Tween 20, disodium hydrogen phosphate 12H 2 O, sodium dihydrogen phosphate dehydrate, dibutylhydroxytoluene (BHT), ammonium acetate, L-tyrosine (Tyr), sodium sulfite (Na 2 SO 3 ), and D-mannitol were purchased from Wako Pure Chemical Industries (Osaka, Japan). Sodium azide (NaN 3 ), L-tryptophan (Trp), L-cysteine (Cys), and L-histidine (His) were purchased from Sigma-Aldrich Japan (Tokyo, Japan). L-Ascorbic acid (vitamin C; VC) was purchased from Nacalai Tesque, Inc. (Kyoto, Japan). Methanol (MeOH) and acetonitrile (liquid chromatography grade) were purchased from Kanto Chemical (Tokyo, Japan).
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