Agilent bioanalyzer with rna 6000 nano kit

Manufactured by Agilent Technologies

The Agilent Bioanalyzer with the RNA 6000 Nano kit is a lab instrument that provides automated, high-resolution analysis of RNA samples. It is designed to assess the quality and quantity of RNA samples in a quick and efficient manner.

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Lab products found in correlation

Nanodrop spectrophotometer, by Thermo Fisher Scientific (2 mentions) Qiazol lysis reagent, by Qiagen (1 mentions) Mirneasy mini kit, by Qiagen (1 mentions) Rnase free dnase, by Qiagen (1 mentions) Paxgene blood rna tube, by Qiagen (1 mentions) Novaseq 6000 s4 flow cell, by Illumina (1 mentions)

Spelling variants of this product

RNA 6000 Nano (61 citations) Bioanalyzer RNA 6000 Nano Kit (51 citations) Agilent RNA 6000 Nano (23 citations) Agilent Bioanalyzer RNA 6000 Nano kit (21 citations) Agilent Bioanalyzer RNA Nano kit (6 citations) RNA 6000 Nano Bioanalyzer (4 citations) RNA 6000 Bioanalyzer Kit (3 citations) RNA Nano 6000 Bioanalyzer Agilent (2 citations) Agilent Bioanalyzer RNA 6000 (2 citations) RNA 6000 Bioanalyzer (2 citations)

2 protocols using agilent bioanalyzer with rna 6000 nano kit

Total RNA Extraction from Mouse Liver

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Total RNA from mouse liver tissue was extracted using the miRNeasy Mini Kit (QIAGEN) and QIAzol Lysis Reagent (QIAGEN) according to the manufacturer's protocol. Extracted RNA was treated with DNase using RNase-Free DNase (QIAGEN). RNA quality was assessed using an Agilent Bioanalyzer with RNA 6000 Nano kit. All samples had RIN score ≥7.

Mononen J., Taipale M., Malinen M., Velidendla B., Niskanen E., Levonen A.L., Ruotsalainen A.K, & Heikkinen S. (2023). Genetic variation is a key determinant of chromatin accessibility and drives differences in the regulatory landscape of C57BL/6J and 129S1/SvImJ mice. Nucleic Acids Research, 52(6), 2904-2923.

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Whole Blood RNA Sequencing Protocol

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Subjects were enrolled during their ED stay, at which time peripheral whole blood was collected in QIAGEN PAXgene Blood RNA tubes, then processed according to manufacturer instructions, and stored at −80ºC. Total RNA was extracted with QIAGEN miRNA kit following all manufacturer instructions. Total RNA quantity and quality were assessed using the Nanodrop spectrophotometer (ThermoScientific) and Agilent Bioanalyzer with RNA 6000 Nano kit. Libraries were prepared using NUGEN Universal Plus mRNA Seq Kit with AnyDeplete globin and rRNA depletion (Tecan Life Sciences) and sequenced using Illumina NovaSeq 6000 S4 flow cell at 150 bp paired-end (PE) with an average of 40M read pairs per RNA sample.

Fiorino C., Liu Y., Henao R., Ko E.R., Burke T.W., Ginsburg G.S., McClain M.T., Woods C.W, & Tsalik E.L. (2022). Host gene expression to predict sepsis progression. Critical care medicine, 50(12), 1748-1756.

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