Fluorescent 488 secondary antibody

Manufactured by Thermo Fisher Scientific

Sourced in United Kingdom

The Fluorescent 488-secondary antibody is a laboratory reagent designed for use in fluorescence-based detection and analysis techniques. It is a secondary antibody conjugated with a fluorescent dye that emits light in the 488 nm wavelength range when excited. This product can be used to detect and visualize target proteins or other biomolecules in various applications such as immunohistochemistry, flow cytometry, and Western blotting.

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Lab products found in correlation

Dapi fluoromount g, by Southern Biotech (2 mentions) Paraformaldehyde (pfa), by Thermo Fisher Scientific (2 mentions) Bovine serum albumin (bsa), by Merck Group (2 mentions) Lsm 510 meta confocal microscope, by Zeiss (2 mentions) Sc 374181, by Santa Cruz Biotechnology (2 mentions)

Spelling variants of this product

Fluorescent secondary antibodies (266 citations) Alexa Fluor 488 fluorescent secondary antibody (6 citations) Alexa 488 fluorescent secondary antibody (2 citations)

2 protocols using fluorescent 488 secondary antibody

Immunofluorescence Staining Protocol

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Cells were fixed with 4% paraformaldehyde (Thermo Fisher Scientific, Cramlington, UK), permeabilized with 0.2% Triton X-100 (T8532, Sigma-Aldrich, Dorset, UK), blocked with 1% bovine serum albumin (Sigma-Aldrich) and incubated overnight with antibodies to PSPC1 (sc-374181, Santa Cruz Biotechnology, Dallas, TX, USA) or NONO (611279, BD Biosciences) followed by fluorescent 488-secondary antibody (Life Technologies, Paisley, UK) before mounting using DAPI Fluoromount G (Southern Biotech, Birmingham, AL, USA). Images were taken using a LSM 510 META confocal microscope (Zeiss, Oberkochen, Germany).

Choudhry H., Albukhari A., Morotti M., Haider S., Moralli D., Smythies J., Schödel J., Green C.M., Camps C., Buffa F., Ratcliffe P., Ragoussis J., Harris A.L, & Mole D.R. (2014). Tumor hypoxia induces nuclear paraspeckle formation through HIF-2α dependent transcriptional activation of NEAT1 leading to cancer cell survival. Oncogene, 34(34), 4482-4490.

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Immunofluorescence Staining of PSPC1 and NONO

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Cells were fixed with 4 % paraformaldehyde (Thermo Fisher Scientific, Cramlington, UK), permeabilized with 0.2 % Triton X-100 (T8532, Sigma-Aldrich, Dorset, UK), blocked with 1 % BSA (Sigma-Aldrich) and incubated overnight with antibodies to PSPC1 (sc-374181, Santa Cruz Biotechnology, Dallas, TX, US), or NONO (611279, BD Biosciences) followed by fluorescent 488-secondary antibody (Life Technologies, Paisley UK) before mounting using DAPI Fluoromount G (Southern Biotech, Birmingham, AL, US). Images were taken using a LSM 510 META confocal microscope (Zeiss, Oberkochen, Germany).

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