K ybgl kit

Pure cultures of Pleurotus eryngii (PE) strain LGAM216 (Basidiomycota, Agaricales) are maintained in the Fungal Culture Collection of the Laboratory of General and Agricultural Microbiology (Agricultural University of Athens). Mushroom cultivation and batch in vitro fermentation were performed as previously described (3 (link), 27 (link)). For the extraction, 50 g of lyophilized PE powder were suspended in 1 L H₂O at 95°C for 24 h, under continuous stirring. The mixture was centrifuged at 4,500 rpm for 1 h; the supernatant was transferred to a new tube and evaporated using a rotary evaporator at 50°C. The concentrated supernatant was lyophilized to dryness, and the semisolid material obtained was kept at 4°C until use. α- and β-glucan content of P. eryngii extract (PE-E) was determined by K-YBGL kit (Megazyme) according to the manufacturer’s instructions. The extraction procedure resulted in an aqueous extract with total glucan content 43 ± 3.3% (w/w), α-glucan content 11 ± 0.7% (w/w), and β-glucan content 32 ± 2.7% (w/w). The lyophilized extract was dissolved in water at appropriate concentrations for the in vivo experiments.

β-Glucan content in PLM was analysed using the K-YBGL kit (Megazyme, Ireland) as the mushroom and yeast β-glucan assay procedure. Extraction, laboratory analysis, and calculation were performed in accordance with the manufacturer's instructions. β-glucan content was calculated as a percentage using the following equation: $\begin{array}{c}\beta -\text{Glucan\hspace{0.17em}content}\left(\%\right)=\left(\text{total\hspace{0.17em}glucan\hspace{0.17em}content}\right)-\left(\text{content\hspace{0.17em}excluding\hspace{0.17em}}\beta -\text{glucan}\right).\end{array}$
Consequently, β-glucan content of PLM was 14.16 ± 6.27%.

β-glucan content in PLM was performed using K-YBGL kit (Megazyme, Ireland) as the mushrooms and yeast β-glucan assay procedure. Extraction, laboratory analysis, and calculation were performed in accordance with the manufacturer’s instructions. The content of β-glucan was calculated using the following formula: $$\mathrm{\beta }-\mathrm{glucan}\mathrm{content}\left(\%\right)=\left(\mathrm{Total},,\mathrm{glucan},,\mathrm{content}\right)-\left(\mathrm{content},,\mathrm{excluding},,\mathrm{\beta },-,\mathrm{glucan}\right)$$
Consequently, β-glucan content of PLM was 14.16 ± 6.27%.