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U0126

Manufactured by AbMole
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U0126 is a chemical compound that functions as a potent and selective inhibitor of the mitogen-activated protein kinase kinase (MEK1/2) enzymes. It is commonly used in cellular and molecular biology research to study the role of the MEK/ERK signaling pathway in various biological processes.

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Lab products found in correlation

Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions) Cleaved caspase 3, by Cell Signaling Technology (2 mentions) Recombinant human trail, by Merck Group (2 mentions) Phospho p38, by Cell Signaling Technology (2 mentions) Fetal bovine serum (fbs), by National Collection of Authenticated Cell Cultures (2 mentions) Rpmi 1640 medium, by National Collection of Authenticated Cell Cultures (2 mentions) Caspase 8, by Cell Signaling Technology (2 mentions) Phospho erk, by Cell Signaling Technology (2 mentions) Caspase 3, by Cell Signaling Technology (2 mentions) Phospho jnk, by Cell Signaling Technology (2 mentions) Caspase 9, by Cell Signaling Technology (2 mentions) Mcl 1, by Cell Signaling Technology (2 mentions) Bcl 2, by Cell Signaling Technology (2 mentions) Cleaved caspase 9, by Cell Signaling Technology (2 mentions) β actin, by Cell Signaling Technology (2 mentions) Ciap1, by Cell Signaling Technology (2 mentions) Ciap2, by Cell Signaling Technology (2 mentions) Goat anti mouse igg hrp abs20001, by Absin (2 mentions) Goat anti rabbit igg hrp abs20002, by Absin (2 mentions) Sp600125, by AbMole (2 mentions)

4 protocols using u0126

1

Magnesium Regulates MAPK Signaling in DPSCs

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To assess MAPK signaling involvement, DPSCs underwent culture in 12-well plates in odontogenic medium with the addition of 0, 1, 5, or 10 mM MgCl2 for 7 days, respectively. Then, total protein from DPSCs was isolated to measure the protein amounts of ERK, p-ERK, JNK, p-JNK, p38, and p-p38 by automated immunoblot. During odontogenic differentiation of DPSCs administered 5 mM MgCl2 for 7 days, ERK/MAPK signaling was suppressed with 50 μM U0126 (AbMole BioScience, USA) for 2 h every 2 days.
Kong Y., Hu X., Zhong Y., Xu K., Wu B, & Zheng J. (2019). Magnesium-enriched microenvironment promotes odontogenic differentiation in human dental pulp stem cells by activating ERK/BMP2/Smads signaling. Stem Cell Research & Therapy, 10, 378.
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2

Signaling Pathway Modulation Assay

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Clofazimine was purchased from Sigma-Aldrich; TBI-166 was provided by the Institute of Materia Medica, Peking Union Medical College, and Chinese Academy of Medical Sciences; lipopolysaccharides from Escherichia coli O55:B5 (L2880) was purchased from Sigma-Aldrich. BAY11-7082 (M2040), SP600125 (M2076), and U0126 (M1977) were purchased from Abmole. Luciferase reporter assay plasmids for pRL-TK, Gal4-Elk, Gal4-Luc, pFA-cJun, RacL61, pNF-κB-luc, and RasV12 were kindly provided by Cuihua Liu (Institute of Microbiology, Chinese Academy of Sciences). The following antibodies were used in this study: anti-p-Jnk (sc-81502, Santa Cruz, USA), anti-Jnk (9252, CST, USA), anti-p-p38 (sc-17852-R, Santa Cruz, USA), anti-p38 (sc-7972, Santa Cruz, USA), anti-p-IκBα (sc-101713, Santa Cruz, USA), anti-IκBα (sc-371; Santa Cruz, USA), p-ERK1/2 (9101, CST, USA), and ERK1/2 (9102, CST, USA).
Li X., Luo X., Wang B., Fu L., Chen X, & Lu Y. (2024). Clofazimine inhibits innate immunity against Mycobacterium tuberculosis by NF-κB. mSphere, 9(8).
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3

TRAIL and 5-FU Combination Therapy

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The GES-1, AGS and HGC27 cell lines were purchased from the cell bank of the Chinese Academy of Sciences and cultured in RPMI-1640 medium containing 10% fetal bovine serum (FBS). The incubator conditions were 37°C and 5% CO 2 (12) . FBS and RPMI-1640 medium were purchased from Gibco (New York, USA). Recombinant human TRAIL (#T9701) and 5-FU (#F6627) were purchased from Sigma-Aldrich (Munich, Germany). The small-molecule inhibitors U0126 (#M1977), SP600125 (#M2076) and SB202190 (#M2062) were obtained from AbMole (Houston, USA). Primary antibodies against the following proteins were purchased from Cell Signaling Technology (Beverly, MA, USA): TRAIL (#3219), Caspase-3 (#9662), Caspase-8 (#9746), Caspase-9 (#9502), Cleaved Caspase-3 (#9661), Cleaved Caspase-8 (#9496), Cleaved Caspase-9 (#9505), PARP (#9532), Bid (#2002), DR4 (#42533), DR5 (#8047), c-IAP1 (#7065), c-IAP2 (#3130), Bcl-2 (#4223), Mcl-1 (#94296), Erk (#4695), JNK (#9252), p38 (#8690), phospho-Erk (#4370), phospho-JNK (#4668), phospho-p38 (#4511) and β-actin (#4970). The secondary antibodies used in this study included goat anti-mouse IgG-HRP (abs20001) and goat anti-rabbit IgG-HRP (abs20002), both of which were obtained from Absin (Shanghai, China).
Li H., Lv J., Guo J., Wang S., Liu S., Ma Y., Liang Z., Wang Y., Qi W, & Qiu W. (2021). 5-Fluorouracil enhances the chemosensitivity of gastric cancer to TRAIL via inhibition of the MAPK pathway. Biochemical and biophysical research communications, 540.
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4

TRAIL and 5-FU Combination Therapy

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The GES-1, AGS and HGC27 cell lines were purchased from the cell bank of the Chinese Academy of Sciences and cultured in RPMI-1640 medium containing 10% fetal bovine serum (FBS). The incubator conditions were 37°C and 5% CO 2 (12) . FBS and RPMI-1640 medium were purchased from Gibco (New York, USA). Recombinant human TRAIL (#T9701) and 5-FU (#F6627) were purchased from Sigma-Aldrich (Munich, Germany). The small-molecule inhibitors U0126 (#M1977), SP600125 (#M2076) and SB202190 (#M2062) were obtained from AbMole (Houston, USA). Primary antibodies against the following proteins were purchased from Cell Signaling Technology (Beverly, MA, USA): TRAIL (#3219), Caspase-3 (#9662), Caspase-8 (#9746), Caspase-9 (#9502), Cleaved Caspase-3 (#9661), Cleaved Caspase-8 (#9496), Cleaved Caspase-9 (#9505), PARP (#9532), Bid (#2002), DR4 (#42533), DR5 (#8047), c-IAP1 (#7065), c-IAP2 (#3130), Bcl-2 (#4223), Mcl-1 (#94296), Erk (#4695), JNK (#9252), p38 (#8690), phospho-Erk (#4370), phospho-JNK (#4668), phospho-p38 (#4511) and β-actin (#4970). The secondary antibodies used in this study included goat anti-mouse IgG-HRP (abs20001) and goat anti-rabbit IgG-HRP (abs20002), both of which were obtained from Absin (Shanghai, China).
Li H., Lv J., Guo J., Wang S., Liu S., Ma Y., Liang Y., Wang Y., Qi W., & Qiu W. (2020). 5-Fluorouracil enhances the chemosensitivity of gastric cancer to TRAIL via inhibition of the MAPK pathway.
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