Cell viability was assessed using the cell counting kit 8 (CCK-8, Sigma-Aldrich) as previously described [28 (link)]. Briefly, 5 × 104 cells/well were seeded into 96-well plates and grown to confluence. Medium was then replaced with 100 µL of fresh DMEM containing increasing concentrations of the samples to the appropriate well. After the incubation time, 10 µL of CCK-8 was added to each well and incubated for 1 h. Absorbance was measured at 450 nm using a MP96 microplate reader spectrophotometer (Safas, Monte Carlo, Principality of Monaco). Treatments were performed in sextuplicate in three independent experiments and cell viability was calculated by normalizing the absorbance of the test wells to the untreated control.
Cck 8
Manufactured by Safas
The CCK-8 (Cell Counting Kit-8) is a colorimetric assay for the determination of cell viability and proliferation. It utilizes a water-soluble tetrazolium salt, which is reduced by cellular dehydrogenases to produce a yellow-colored formazan dye. The amount of formazan dye generated is directly proportional to the number of viable cells present in the sample.
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Cell Viability Assay with CCK-8
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Cell Viability Assay with CCK-8
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Cell viability was assessed by using CCK-8 (Sigma-Aldrich) assay, following the supplier instructions. 5x10 3 cells/well were seeded into 96-well plates and grown to confluence. Medium was then replaced with 100 µl of fresh RPMI containing treatments to the appropriate well. After the incubation time, 10 µl of CCK-8 was added to each well and incubated for 1 h. Absorbance was measured at 450 nm using a MP96 microplate reader spectrophotometer (Safas, Monte Carlo, Principality of Monaco). Treatments were performed in sextuplicate in three independent experiments and cell viability was calculated by normalizing values to the untreated control.
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