α abc

Manufactured by Cell Signaling Technology

Sourced in United Kingdom

α-ABC is an antibody product offered by Cell Signaling Technology. It is designed to detect the target protein of interest.

Automatically generated - may contain errors

Lab products found in correlation

α flag, by Merck Group (2 mentions) α flag m2 affinity gel, by Merck Group (1 mentions) α β actin, by Merck Group (1 mentions) α bira, by Abcam (1 mentions) Gfp trap resin, by Proteintech (1 mentions) α β actin, by Abcam (1 mentions) α gfp, by Merck Group (1 mentions) α β catenin, by BD (1 mentions) α actin, by Abcam (1 mentions)

2 protocols using α abc

Plasmids, Antibodies, and Resins for Wnt Signaling

Cited 3 times

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The following plasmids have been described: LDB1-FLAG, SSDP-FLAG (Fiedler et al., 2015 (link)); murine FLAG-B9L, FLAG-ΔC (called FLAG-ΔCter), GFP-B9L (Adachi et al., 2004 (link)); GFP-Lgs, GFP-TCF4 (Townsley et al., 2004 (link)). Human GFP-BCL9 was generated by exchanging the FLAG tag of FLAG-BCL9 (de la Roche et al., 2008 (link)). Mutants were made from parental plasmids using standard site-directed mutagenesis procedures, and confirmed by sequencing. TLE3 was amplified by PCR from 6xMyc-TLE3 (Hanson et al., 2012 (link)) and subcloned in pcDNA3.1-N-HA, and mutants (WD40, ΔWD40) were generated from this subclone.
The following antibodies and resins were used: α-GFP RRID:AB_439690, α-FLAG RRID:AB_262044, α-HA RRID:AB_390918, α-β-actin (for human lysates) RRID:AB_476744 (Sigma-Aldrich, St. Louis MO, USA); α-BCL9 RRID:AB_2063609, α-BCL9-2 RRID:AB_2063747 (Abingdon, UK); α-β-actin (for fly lysates) RRID:AB_2305186, α-BirA RRID:AB_300830, α-Pygo2 RRID:AB_10863482 (Abcam, Cambridge, UK); α-ABC RRID:AB_11127203 (Cell Signaling Technology). Rat α-Lgs antiserum (Eurogentec, Liège, Belgium) was obtained from pre-bleed immunizations with gluthathione S-transferase-purified Lgs_232-555. GFP-Trap resin RRID:AB_2631357 (Chromotek, Planegg, Germany) was used for coIP assays, and α-FLAG M2 affinity gel RRID:AB_10063035 (Sigma-Aldrich) was used for RIME.

van Tienen L.M., Mieszczanek J., Fiedler M., Rutherford T.J, & Bienz M. (2017). Constitutive scaffolding of multiple Wnt enhanceosome components by Legless/BCL9. eLife, 6, e20882.

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Wnt Signaling Pathway Analysis with Reagents

Cited 1 time

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The following reagents were used: FLAG-β-catenin, TCF1(p45) (provided by H. Clevers, Hubrecht Institute, Utrecht); TOP-GFP/CMV-dsRFP (provided by C. Gottardi, Northwestern University Feinberg School of Medicine, Chicago); FLAG-BCL9, FLAG-BCL9^L366K, FLAG-BCL9ΔC, FLAG-BCL9ΔC^L366K (22 (link)); pcDNA-Myc-TCF4 (23 (link)); pcDNAHA-LEF1; SuperTOP (24 (link)); dimethyl sulphoxide (DMSO), CA, XAV939, IWR-1, pyrvinium pamoate, iCRT3 (Sigma Pharmaceuticals); PKF115-584 (Enzo Life Sciences); α-β-catenin (BD Transduction Laboratories); α-ABC, α-PBC (phospho-Ser33/37/Thr41), α-LEF1 (C18A7), α-TCF1, α-TCF4 (Cell Signaling); α-BCL9, α-B9L (R&D Systems); α-actin (Abcam); α-FLAG (Sigma-Aldrich). ICG-001 and 16k were synthesized by MRC Technology.

de la Roche M., Ibrahim A.E., Mieszczanek J, & Bienz M. (2014). LEF1 and B9L shield β-catenin from inactivation by Axin, desensitizing colorectal cancer cells to tankyrase inhibitors. Cancer research, 74(5), 1495-1505.

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