Parasitized cell lines were generated by in vitro infection of bovine PBMCs obtained from the calves before ITM immunisation (39 (link)). Cell lines were maintained at 37°C under 5% CO2 in RPMI-1640 Glutamax cell culture medium (Life Technologies, Gibco, Paisley, UK) supplemented with 10% FCS (GIBCO), penicillin-streptomycin (100 units mL-1 and 100 µg mL-1, final concentrations respectively, Sigma-Aldrich, Dorset, UK) and 50 μM (final concentration) of 2-mercaptoethanol (Sigma Aldrich).
Rpmi 1640 glutamax cell culture medium
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom
RPMI-1640 Glutamax is a cell culture medium designed to support the growth and maintenance of a variety of cell lines. It is a modified version of the original RPMI-1640 medium, with the addition of glutamine to enhance cell growth and performance.
Automatically generated - may contain errors
2 protocols using rpmi 1640 glutamax cell culture medium
1
Generating Parasitized Cell Lines
2
Bovine PBMC Culture for Parasitized Cells
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Parasitized cell lines were generated by in vitro infection of bovine PBMCs obtained from the calves before ITM immunisation 38 . Cell lines were maintained at 37°C under 5% CO2 in RPMI-1640 Glutamax cell culture medium (Life Technologies, Gibco, Paisley, UK) supplemented with 10% FCS (GIBCO), penicillin-streptomycin (100 units mL -1 and 100 µg mL -1 , final concentrations respectively, Sigma-Aldrich, Dorset, UK) and 50 μM (final concentration) of 2-mercaptoethanol (Sigma Aldrich).
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