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Ziptip c4 micro columns

Manufactured by Merck Group
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The ZipTip® C4 micro-columns are a type of sample preparation device used in analytical chemistry. They are designed to extract, concentrate, and purify analytes from complex samples prior to analysis. The ZipTip® C4 micro-columns contain a stationary phase of C4 reversed-phase material, which allows for the selective retention and elution of specific compounds based on their hydrophobic interactions.

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Lab products found in correlation

Flexanalysis 3, by Bruker (6 mentions) Protein 1 standard calibration mixture, by Bruker (5 mentions) Ground steel massive 384 target plate, by Bruker (3 mentions) Autoflex 3, by Bruker (3 mentions) Groundsteel massive 384 target, by Bruker (3 mentions) Autoflex 3 maldi tof tof spectrometer, by Bruker (3 mentions) Flexcontrol 3, by Bruker (3 mentions) Protein calibration standard 1, by Bruker (1 mentions)

6 protocols using ziptip c4 micro columns

1

MALDI-TOF Protein Desalting and Analysis

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A 2 μl protein sample was desalted using ZipTip® C4 micro-columns (Millipore) and eluted with 0.5 μl SA (sinapinic acid, 10 mg/ml in [70:30] Acetonitrile: Trifluoroacetic acid 0.1%) matrix onto a GroundSteel massive 384 target (Bruker Daltonics, Billerica, MA, USA). An Autoflex III MALDI-TOF/TOF spectrometer (Bruker Daltonics) was used in linear mode with the following settings: 5000–40000 Th window, linear positive mode, ion source 1: 20 kV, ion source 2: 18.5 kV, lens: 9 kV, pulsed ion extraction of 120 ns, high gating ion suppression up to 1000 Mr. Mass calibration was performed externally with protein 1 standard calibration mixture (Bruker Daltonics). Data acquisition, peak peaking and subsequent spectra analysis was performed using FlexControl 3.0 and FlexAnalysis 3.0 software (Bruker Daltonics).
Alvarez-Cienfuegos A., Nuñez-Prado N., Compte M., Cuesta A.M., Blanco-Toribio A., Harwood S.L., Villate M., Merino N., Bonet J., Navarro R., Muñoz-Briones C., Sørensen K.M., Mølgaard K., Oliva B., Sanz L., Blanco F.J, & Alvarez-Vallina L. (2016). Intramolecular trimerization, a novel strategy for making multispecific antibodies with controlled orientation of the antigen binding domains. Scientific Reports, 6, 28643.
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2

MALDI-TOF/TOF Mass Spectrometry of Fab

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Prior to the measurement, all Fab preparations were desalted using ZipTip® C4 micro-columns (Millipore) (2 μL sample) with 0.5 μL SA buffer (sinapinic acid, 10 mg/ml in [70:30] Acetonitrile:Trifluoroacetic acid 0.1%), and arrayed onto a Ground Steel massive 384 target plate (Bruker Daltonics). Mass determinations were performed in a matrix-assisted laser desorption/ionization (MALDI), tandem time-of-flight (TOF/TOF) spectrometer Autoflex III (Bruker Daltonics). Mass calibration was performed externally with a Protein Calibration Standard 1 mixture (Bruker Daltonics) in the same mass range as the samples. Data acquisition, peak peaking and subsequent spectra analysis were performed using flexAnalysis 3.0 software (Bruker Daltonics).
Rujas E., Insausti S., Leaman D.P., Carravilla P., González-Resines S., Monceaux V., Sánchez-Eugenia R., García-Porras M., Iloro I., Zhang L., Elortza F., Julien J.P., Saéz-Cirión A., Zwick M.B., Eggeling C., Ojida A., Domene C., Caaveiro J.M, & Nieva J.L. (2020). Affinity for the Interface Underpins Potency of Antibodies Operating In Membrane Environments. Cell reports, 32(7), 108037.
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3

MALDI-TOF Analysis of Conjugated Antibodies

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Prior to the measurement, conjugated antibodies were desalted using ZipTip® C4 micro columns (Millipore) (2 μL sample) with 0.5μL SA buffer (sinapinic acid, 10 mg/mL in [70:30] Acetonitrile:Trifluoroacetic acid 0.1%), and spotted onto a Ground Steel massive 384 target plate (Bruker Daltonics). Mass determination was performed by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) analysis in an Autoflex III (Bruker Daltonics). Mass calibration was performed externally with a Protein Calibration Standard 1 (Bruker Daltonics) mixture in the same mass range as the samples. Data acquisition, peak peaking and subsequent spectra analysis were performed using flexAnalysis 3.0 software (Bruker Daltonics).
Rujas E., Leaman D.P., Insausti S., Carravilla P., García-Porras M., Largo E., Morillo I., Sánchez-Eugenia R., Zhang L., Cui H., Iloro I., Elortza F., Julien J.P., Eggeling C., Zwick M.B., Caaveiro J.M, & Nieva J.L. (2021). Focal accumulation of aromaticity at the CDRH3 loop mitigates 4E10 polyreactivity without altering its HIV neutralization profile. iScience, 24(9), 102987.
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4

MALDI-TOF/TOF Mass Spectrometry of Fab

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Prior to the measurement, all Fab preparations were desalted using ZipTip® C4 micro-columns (Millipore) (2 μL sample) with 0.5 μL SA buffer (sinapinic acid, 10 mg/ml in [70:30] Acetonitrile:Trifluoroacetic acid 0.1%), and arrayed onto a Ground Steel massive 384 target plate (Bruker Daltonics). Mass determinations were performed in a matrix-assisted laser desorption/ionization (MALDI), tandem time-of-flight (TOF/TOF) spectrometer Autoflex III (Bruker Daltonics). Mass calibration was performed externally with a Protein Calibration Standard 1 mixture (Bruker Daltonics) in the same mass range as the samples. Data acquisition, peak peaking and subsequent spectra analysis were performed using flexAnalysis 3.0 software (Bruker Daltonics).
Rujas E., Insausti S., Leaman D.P., Carravilla P., González-Resines S., Monceaux V., Sánchez-Eugenia R., García-Porras M., Iloro I., Zhang L., Elortza F., Julien J.P., Saéz-Cirión A., Zwick M.B., Eggeling C., Ojida A., Domene C., Caaveiro J.M, & Nieva J.L. (2020). Affinity for the Interface Underpins Potency of Antibodies Operating In Membrane Environments. Cell reports, 32(7), 108037.
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5

MALDI-TOF Protein Desalting and Analysis

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A 10 μl protein sample was desalted using ZipTip® C4 micro-columns (Merck Millipore, Burlington, MS, USA) and eluted with 0.5 μl SA [sinapinic acid, 10 mg/ml in [70:30] Acetonitrile: Trifluoroacetic acid 0.1%] matrix onto a GroundSteel massive 384 target (Bruker Daltonics, Billerica, MA, USA). An Autoflex III MALDI-TOF/TOF spectrometer (Bruker Daltonics) was used in linear mode with the following settings: 5,000–40,000 Th window, linear positive mode, ion source 1: 20 kV, ion source 2: 18.5 kV, lens: 9 kV, pulsed ion extraction of 120 ns, high gating ion suppression up to 1,000 Mr. Mass calibration was performed externally with protein 1 standard calibration mixture (Bruker Daltonics). Data acquisition, peak peaking and subsequent spectra analysis was performed using FlexControl 3.0 and FlexAnalysis 3.0 software (Bruker Daltonics).
Mikkelsen K., Harwood S.L., Compte M., Merino N., Mølgaard K., Lykkemark S., Alvarez-Mendez A., Blanco F.J, & Álvarez-Vallina L. (2019). Carcinoembryonic Antigen (CEA)-Specific 4-1BB-Costimulation Induced by CEA-Targeted 4-1BB-Agonistic Trimerbodies. Frontiers in Immunology, 10, 1791.
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6

MALDI-TOF/TOF Analysis of Protein Samples

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A 2 μl protein sample was desalted using ZipTip® C4 micro-columns (Merck Millipore) and eluted with 0.5 μl sinapinic acid (10 mg/ml in [70:30] Acetonitrile: Trifluoroacetic acid 0.1%) matrix onto a GroundSteel massive 384 target (Bruker Daltonics). An Autoflex III MALDI-TOF/TOF spectrometer (Bruker Daltonics) was used in linear mode with the following settings: 5000–40,000 Th window, linear positive mode, ion source 1: 20 kV, ion source 2: 18.5 kV, lens: 9 kV, pulsed ion extraction of 120 ns, high gating ion suppression up to 1000 Mr. Mass calibration was performed externally with protein 1 standard calibration mixture (Bruker Daltonics). Data acquisition, peak peaking, and subsequent spectra analysis was performed using the FlexControl 3.0 and FlexAnalysis 3.0 software (Bruker Daltonics).
Compte M., Harwood S.L., Muñoz I.G., Navarro R., Zonca M., Perez-Chacon G., Erce-Llamazares A., Merino N., Tapia-Galisteo A., Cuesta A.M., Mikkelsen K., Caleiras E., Nuñez-Prado N., Aznar M.A., Lykkemark S., Martínez-Torrecuadrada J., Melero I., Blanco F.J., Bernardino de la Serna J., Zapata J.M., Sanz L, & Alvarez-Vallina L. (2018). A tumor-targeted trimeric 4-1BB-agonistic antibody induces potent anti-tumor immunity without systemic toxicity. Nature Communications, 9, 4809.
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