Log In Sign up
The largest database of trusted experimental protocols

Infinity cholesterol kit

Manufactured by Thermo Fisher Scientific
Visit Supplier
Sourced in United Kingdom

The Infinity Cholesterol Kit is a laboratory test used to measure the total cholesterol levels in a sample. It provides a quantitative determination of cholesterol concentration through a colorimetric reaction.

Automatically generated - may contain errors

Lab products found in correlation

Accu chek glucometer, by Roche (1 mentions) Ultrasensitive mouse insulin enzyme linked immunosorbent assay kit, by Crystal Chem (1 mentions) Multiconstituent calibrator 1e65 04, by Abbott (1 mentions) Hydroxyproline colorimetric assay kit, by Merck Group (1 mentions) Au480 clinical chemistry analyzer, by Beckman Coulter (1 mentions) Infinity triglyceride, by Thermo Fisher Scientific (1 mentions) Alt colorimetric activity assay kit, by Cayman Chemical (1 mentions) Hydroxyproline assay kit, by Cell Biolabs (1 mentions) Total bilirubin reagent set, by Horiba (1 mentions) Onetouch ultra, by LifeScan (1 mentions) Synergy ht, by Agilent Technologies (1 mentions) Enzyme linked immunosorbent assay kit, by Merck Group (1 mentions) Phospholipid c, by Fujifilm (1 mentions) Nefa hr kit, by Fujifilm (1 mentions) Bca kit, by Thermo Fisher Scientific (1 mentions) Tissue tearor, by Biospec (1 mentions) Onetouch ultramini blood glucose meter, by LifeScan (1 mentions) Triglyceride colorimetric assay kit, by Cayman Chemical (1 mentions) Total bile acid assay kit, by Cell Biolabs (1 mentions) Cobas c311 system, by Roche (1 mentions)

Spelling variants of this product

Cholesterol (73 citations) Infinity kit (30 citations) Infinity Cholesterol (20 citations) Infinity cholesterol assay kits (6 citations) Infinity triglyceride and cholesterol kits (4 citations)

12 protocols using infinity cholesterol kit

1

Liver and Metabolic Biomarkers Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Plasma albumin, bilirubin, blood urea nitrogen, creatinine, alanine aminotransferase (ALT), and aspartate aminotransferase (AST) were measured using an AU480 Clinical Chemistry Analyzer (Beckman Coulter, Providence, RI). Basal blood glucose and plasma insulin were assessed using an Accu‐Chek glucometer (Roche Diagnostics, Basel, Switzerland) and the Ultrasensitive Mouse Insulin Enzyme‐Linked Immunosorbent Assay kit (Crystal Chem, Downers Grove, IL), respectively. Plasma levels of TAG and cholesterol were measured using the Infinity TAG and the Infinity Cholesterol kits, respectively (Thermo Fisher Scientific, Waltham, MA), with Multiconstituent Calibrator 1E65‐04 (Abbott, North Chicago, IL) used as reference. For hepatic TAG measurements, lipids were extracted from liver samples and analyzed using straight‐phase high‐performance liquid chromatography as described.21 Hepatic collagen deposition was assessed in liver homogenates using the Hydroxyproline Colorimetric Assay kit (Sigma‐Aldrich, St. Louis, MO).
Nuñez‐Durán E., Aghajan M., Amrutkar M., Sütt S., Cansby E., Booten S.L., Watt A., Ståhlman M., Stefan N., Häring H., Staiger H., Borén J., Marschall H, & Mahlapuu M. (2017). Serine/threonine protein kinase 25 antisense oligonucleotide treatment reverses glucose intolerance, insulin resistance, and nonalcoholic fatty liver disease in mice. Hepatology Communications, 2(1), 69-83.
+ Open protocol
+ Expand
2

Comprehensive Metabolic Profile Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Non-fasting blood glucose levels were measured using a glucose meter (One Touch Ultra, Life Scan). Total plasma levels of TG, cholesterol, ALT, and bilirubin were measured in colorimetric analyses using Infinity Triglycerides (ThermoFisher Scientific, TR2241) and Infinity Cholesterol Kits (ThermoFisher Scientific, TR-13421), ALT colorimetric activity assay kit (Cayman Chemical, 700260) and a total bilirubin reagent set (Pointe Scientific, 23-666-150) according to the manufacturer’s instructions, respectively. Hydroxyproline assay to quantify collagen content was performed using Hydroxyproline Assay Kit (Cell Biolabs, Inc, STA-675) according to the manufacturer’s instruction. Data were normalized to liver weights.
Xu C., Zhou H., Jin Y., Sahay K., Robicsek A., Liu Y., Dong K., Zhou J., Barrett A., Su H, & Chen W. (2022). Hepatic neddylation deficiency triggers fatal liver injury via inducing NF-κB-inducing kinase in mice. Nature Communications, 13, 7782.
+ Open protocol
+ Expand
3

Plasma TG and Cholesterol Determination

Check if the same lab product or an alternative is used in the 5 most similar protocols
Plasma TG and cholesterol (CHOL) were determined using Randox triglyceride kits (Antrim, UK) and Infinity cholesterol kits (Thermo Electron, Noble Park, Victoria, Australia), respectively.
Zhan J., Weng J., Hunt B.G., Davidson W.S., Liu M, & Lo C.C. (2018). Apolipoprotein A-IV enhances cholecystokinin secretion. Physiology & behavior, 188, 11-17.
+ Open protocol
+ Expand
4

Murine Metabolic Profiling by EchoMRI

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Fat and lean body masses were determined using an EchoMRI whole body composition analyzer (Houston, TX) [39 (link), 41 (link)]. After 10 wk on LFD or the HFD, fat pads, liver and plasma were carefully collected and weighed from 5-h fasted mice. Glucose level was determined in tail blood using a Freestyle glucometer (Abbot Diabetes Care, Alameda, CA). Hepatic lipids were extracted using the Folch method [42 (link)]. Triacylglycerides (TG) and cholesterol (CHOL) in the plasma and the liver were determined using Randox triglyceride kits (Antrim, UK) and Infinity cholesterol kits (Thermo Electron, Noble Park, Victoria, Australia), respectively. According to the manufacturer’s protocol, diluted plasma was mixed with 200 µl enzyme reagent and then incubated at 37° C for 30 min [11 (link)]. The absorbance was read at 500 nm using a microplate reader (Synergy HT; BioTek Instruments, Richmond, VA). Plasma insulin and leptin were determined using commercial enzyme-linked immunosorbent assay kits (Millipore, St. Charles, MO)[11 (link)]. Briefly, 10-µl samples were added to each well of a precoated microtiter plate, and the detection antibody was added to the captured molecules. After incubation, absorbance was measured with a microplate reader and the final concentrations were calculated using standards provided with the enzyme-linked immunosorbent assay kits.
King A., Yang Q., Huesman S., Rider T, & Lo C.C. (2015). Lipid transport in cholecystokinin knockout mice. Physiology & behavior, 151, 198-206.
+ Open protocol
+ Expand
5

Plasma Lipid Profiling in Fasted Animals

Check if the same lab product or an alternative is used in the 5 most similar protocols
Eight weeks postoperatively, plasma lipids were determined in ad libitum–fed animals just prior to the onset of dark; food was removed, and blood was sampled again after a 24-h fast. Blood was collected from the tail in heparin-coated microtubes and spun for 5 min. Plasma triglyceride and cholesterol levels were determined using Randox TG kits (Randox Laboratories, Crumlin, U.K.) and Infinity cholesterol kits (Thermo Electron, Noble Park, Victoria, Australia), respectively. Plasma phospholipids and nonesterified fatty acids were analyzed using phospholipid C and HR Series NEFA-HR(2) kits (Wako Diagnostics), respectively.
Pressler J.W., Haller A., Sorrell J., Wang F., Seeley R.J., Tso P, & Sandoval D.A. (2014). Vertical Sleeve Gastrectomy Restores Glucose Homeostasis in Apolipoprotein A-IV KO Mice. Diabetes, 64(2), 498-507.
+ Open protocol
+ Expand
6

Quantification of Cellular Cholesterol and FFAs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total cholesterol and free fatty acids (FFAs) were also measured in steatotic and non-steatotic WIF-B9 cells after two days of treatment with the FA mixture or not. Cholesterol quantification was performed by the Infinity cholesterol kit (Thermo Fisher Scientific, Cergy Pontoise, France), according to the manufacturer’s instructions. Briefly, 200 µL of reaction solution was added to each sample for 30 minutes at 37 °C, and absorbance at 492 nm was then measured using a Spectrostar Nano microplate reader. Regarding FFA quantification, it was performed by the NEFA-HR kit (Wako Chemicals GmbH, Neuss, Germany) according to the manufacturer’s instructions. After addition of the reaction solution and incubation at 37 °C, absorbance at 546 nm and 660 nm was measured using a Spectrostar Nano microplate reader. FFA concentration was determined after normalization (Δ abs = abs at 546 nm minus abs at 660 nm).
Bucher S., Tête A., Podechard N., Liamin M., Le Guillou D., Chevanne M., Coulouarn C., Imran M., Gallais I., Fernier M., Hamdaoui Q., Robin M.A., Sergent O., Fromenty B, & Lagadic-Gossmann D. (2018). Co-exposure to benzo[a]pyrene and ethanol induces a pathological progression of liver steatosis in vitro and in vivo. Scientific Reports, 8, 5963.
+ Open protocol
+ Expand
7

Murine PCSK9 ELISA Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
A murine PCSK9 enzyme-linked immunosorbent assay was performed according to manufacturer’s instructions (R&D Systems, Inc.; Minneapolis MN, USA). Blood for ELISA was collected via cardiac puncture at the time of sacrifice and plasma prepared by centrifugation at 8500 rpm for 10 min. For cell-associated PCSK9, the pelleted erythrocytes were washed, then lysed with distilled water. Livers were dissected, flash frozen, and then homogenized using a Tissue Tearor (Biospec Products Inc, Bartlesville, OK). Protein concentration of erythrocyte and liver lysates were determined via BCA kit (Thermo Fisher Scientific, Waltham, MA, USA). ELISA values were normalized to protein concentration for each sample. Total cholesterol was measured using the Infinity Cholesterol kit using Data-Trol Normal Control as a known control value (both from Thermo Fisher Scientific, Waltham, MA, USA). Commercial kits were used to assess LDL and HDL cholesterol and compared to the HDL/LDL Cholesterol Calibrator Set (all from Diazyme Laboratories, Poway, CA, USA).
Venugopal J., Wang J., Guo C., Lu H., Chen Y.E, & Eitzman D.T. (2020). Non-hematopoietic deficiency of proprotein convertase subtilisin/kexin type 9 deficiency leads to more severe anemia in a murine model of sickle cell disease. Scientific Reports, 10, 16514.
+ Open protocol
+ Expand
8

Metabolic Evaluation After Vascular Injury

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fasting and random blood glucose were measured via the tail vein once a week after femoral artery wire injury using a glucometer (OneTouch UltraMini Blood Glucose Meter, LifeScan Canada Ltd, Quebec, QC, Canada.) and values were converted to plasma glucose assuming a normal hematocrit. For the measurement of fasting glucose levels, mice had no access to food for a duration of four hours. Insulin-treated mice were allowed 20% glucose water for the initial two hours of fasting to avoid hypoglycemia. In addition, blood samples were collected via cardiac puncture at the time of sacrifice for analysis of metabolic parameters, such as plasma insulin (ELISA obtained from ALPCO, Salem, NH, USA, Catalog #80-INSMS-E01, E10), insulin-like growth factor 1 (IGF-1) (ELISA obtained from R&D systems, Minneapolis, MN, USA, Catalog #MG100), vascular endothelial growth factor (VEGF) (ELISA obtained from R&D systems, Minneapolis, MN, USA, Catalog #MMV00-1), triglyceride (TG), free fatty acids (FFAs) (both colorimetric assay technique, Wako Diagnostics, Osaka, Japan), and total cholesterol (Infinity Cholesterol Kit obtained from Thermo Scientific, Waltham, MA, USA, Catalog #TR13421).
Gonzalez Medina M., Liu Z., Wang J., Zhang C., Cash S.B., Cummins C.L, & Giacca A. (2024). Cell-Specific Effects of Insulin in a Murine Model of Restenosis Under Insulin-Sensitive and Insulin-Resistant Conditions. Cells, 13(16).
+ Open protocol
+ Expand
9

Serum Cholesterol and Triglyceride Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mice were fasted for 4 h prior to tail bleed (or cardiac puncture at experimental endpoint). Blood was left at room temperature for 30 min to allow it to clot and then centrifuged at 857 g for 10 min to allow phase separation. The top opaque yellow phase (serum) was collected, aliquoted, and stored at −20°C. Serum was thawed and analyzed accordingly by the commercially available Infinity-cholesterol™ kit (Thermo Fisher Scientific) and a triglyceride colorimetric assay kit (Cayman Chemical) as per the manufacturer’s instructions.
LeBlond N.D., Ghorbani P., O’Dwyer C., Ambursley N., Nunes J.R., Smith T.K., Trzaskalski N.A., Mulvihill E.E., Viollet B., Foretz M, & Fullerton M.D. (2020). Myeloid deletion and therapeutic activation of AMPK do not alter atherosclerosis in male or female mice. Journal of Lipid Research, 61(12), 1697-1706.
+ Open protocol
+ Expand
10

Bile Acid and Lipid Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
BA levels in plasma were analyzed by LC-MS, and total BAs in feces were measured by using the Total Bile Acid Assay Kit (Cell Biolabs, San Diego, CA, USA) according to manufacturer instructions. Plasma alanine transaminase, aspartate transaminase, HDL cholesterol, LDL-C, and total cholesterol were measured by using Cobas c311 system (Roche Diagnostics). Liver and fecal content of cholesterol and triglycerides was measured from lipid extracts as previously described by using an Infinity Cholesterol Kit (Thermo Fisher Scientific) and LabAssay Triglyceride Kit, respectively.
Chevre R., Trigueros-Motos L., Castaño D., Chua T., Corlianò M., Patankar J.V., Sng L., Sim L., Juin T.L., Carissimo G., Ng L.F.P., Yi C.N.J., Eliathamby C.C., Groen A.K., Hayden M.R, & Singaraja R.R. (2018). Therapeutic modulation of the bile acid pool by Cyp8b1 knockdown protects against nonalcoholic fatty liver disease in mice. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 32(7).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!